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11. Appendix B–Sample Collection and Handling
The YSI 1500 SPORT is not intended for home use. Proper sample collection
and preparation, by a professional, are vital factors in obtaining reliable results
with the YSI SPORT. This section covers the basic guidelines for collecting and
preparing whole blood, plasma and serum samples.
Much of the Blood Plasma and Blood Serum material is abstracted from
“Workshop Manual of Methods for the Determination of Glucose” by Gerald R.
Cooper, M.D., Ph.D. published by the Commission on Continuing Education,
Council on Clinical Chemistry, American Society of Clinical Pathologists
(1966).
Blood Plasma
Blood Plasma
Blood Plasma
Blood Plasma
Be sure that all collection equipment is sterile and chemically clean. Use aseptic
techniques in handling the sample from collection through assay.
During or immediately after collection, mix the specimen with an anticoagulant
and preservative to prevent coagulation, inhibit enzyme activity and prevent
bacterial growth. Separate the red blood cells by centrifugation within 30
minutes of collection. If delays between collection and assay are unavoidable,
follow one of these procedures:
1. Refrigerate the whole blood at +4°C. This is acceptable for periods up to
four hours.
2. Or, refrigerate the plasma with preservatives at +4°C for up to three days.
Plasma with preservative left at room temperature may keep up to four
hours.
3. Or, freeze the cell-free plasma with preservative at -10°C or colder. Plasma
will keep indefinitely at this temperature. Thaw the frozen plasma rapidly at
25°C and analyze promptly. The freeze-thaw cycle should not be repeated.
Tubes containing cells should never be frozen.
NOTE:
NOTE:
NOTE:
NOTE: Chilling or freezing may precipitate aggluten or fibrinogen masses
which may not redissolve. In such cases, remove them by centrifugation before
assay.
Several common anticoagulants are suitable for use with specimens being
prepared for assay. These include:
Potassium oxalate in a concentration of 2 mg/ml of blood.
Sodium fluoride in a concentration of 5-10 mg/ml of blood.
EDTA in a concentration of 1 mg/ml of blood.
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