SpectroArt 200
Spectrophotometer
Installation and Operation Manual
Version 1.0
Item# 03070
*This instrument is intended for laboratory use only
Страница 1: ...SpectroArt 200 Spectrophotometer Installation and Operation Manual Version 1 0 Item 03070 This instrument is intended for laboratory use only ...
Страница 2: ...pectroArt 200 Spectrophotometer Hardware Overview C Installation of SpectroArt 200 Spectrophotometer 7 C 1 Package List C 2 Environment requirement C 3 SpectroArt 200 spectrophotometer setup C 3 1 Calibration and Wavelength Linearity Test C 3 2 UV Parameters C 3 3 Touch Pad Settings C 3 4 Printer Test C 3 5 Set Default C 3 6 Beep Settings C 3 7 Time Date Settings C 3 8 Display Settings D Quick Gui...
Страница 3: ...i D 2 Protein D 3 Kinetics D 4 OD600 D 5 Wavelength D 6 Scan D 7 Functions D 7 1 DNA RNA D 7 2 Protein D 7 3 Kinetics D 7 4 OD600 D 7 5 Wavelength D 7 6 Scan D 7 7 Functions E Care and Maintenance 22 F Order Information 25 ...
Страница 4: ...ophotometer is warranted to be free from defects in materials or workmanship for a period of one year from the original invoice date under normal usage Any defects occurring during warranty period Wealtec Corp will repair or replace defective products or parts without charge unless the defects arise from conditions outlined below The defects described below are specially excluded from Wealtec warr...
Страница 5: ...rference Any modification or alteration will void the warranty void the regulatory certifications and create potential safety hazards A 3 2 Safety Information Before operating the unit please note the following precautions Do not use near flammable materials Always inspect the SpectroArt 200 spectrophotometer for damaged components before use Always connect the system to the correct AC power sourc...
Страница 6: ... SpectroArt 200 is a xenon flash light In contrast to traditional light sources such as deuterium or tungsten halogen that emit either UV light or visible light receptively xenon flash lights emit light of both UV and visible light wavelengths The concentration of a solute can be calculated by measuring either the light that is absorbed by the sample or the light that is transmitted through the sa...
Страница 7: ...3 Absorbance range AU 0 3 0 Data collection speed msec 60 Path length mm 10 Beam length Z axis mm 8 5 Pre set wavelength 9 Max Sample test repetition 10 Max System optimization On start up LCD sleep mode Yes Standard curve storage 10 Max Analytic data storage 99 Max Kinetic analysis reading interval range sec 2 999 Communication port RS232 Number of cuvette 1 Applicable cuvette types Standard Semi...
Страница 8: ...erview Integrated thermal printer Touch screen TFT LCD Cuvette chamber Light path axis Thermal printer paper Printer paper shaft Sample compartment lid Printer paper compartment lid Prog Diagnostic switch Power input module Serial output port RS 232 Fan guard Power switch Prog mode Diagnostic mode ...
Страница 9: ...ent lid Open the printer paper compartment lid to install new roll of printer paper Fan guard Exhaust air leaves cabinet through this opening Universal power input module Appropriate power cord is connected to the power input module Power switch Instrument main power switch Serial output port RS 232 connection to a computer Cuvette chamber Insert cuvette into the cuvette chamber Insure that the cu...
Страница 10: ...ctroArt 200 V1 0 7 C Installation of SpectroArt 200 Spectrophotometer C 1 Package List Item Quantity SpectroArt 200 spectrophotometer 1 Thermal paper roll 1 Power cable 1 Instruction and operation manual 1 ...
Страница 11: ...SpectroArt 200 Spectrophotometer Setup SpectroArt 200 allows you to perform the following in the setup menu Calibration Adjusting UV parameters Restoring touch pad settings Printer testing Beep volume adjustments Time and Date settings Display settings brightness and contrast adjustments Set default settings To enter the setup menu touch the Setup button in the main menu C 3 1 Calibration and Wave...
Страница 12: ...st touch the Protein button in the main menu Touch the Setup button to enter the protein setup menu Touch the Method button five times for customised assay Then touch the Change W length button and key in 280 nm using the number pad Touch the Blank Rep button and enter the number 5 using the number pad Then touch Enter Touch the STD Rep button and enter the number 5 using the number pad Then touch...
Страница 13: ...ouch Enter and then OK Repeat the procedure 4 times until the sample has been read 5 times all together The results from the standard curve are displayed in the protein menu with R2 value and linear equation The R2 value should be 0 98 Touch the Unknown button and enter the number 1 using the number pad Touch Enter Insert the 60 µg ml reference sample in the cuvette holder and touch OK Repeat 4 ti...
Страница 14: ...n altered To perform TP setup a password is required Touch TP Setup in the system setup menu Enter the password using the number pad To delete a number touch the Back button Touch Enter and follow the instructions given 1 Touch the lower left marker 2 Touch the upper right marker 3 Touch the marker labelled 1 4 Touch the marker labelled 2 5 Touch the marker labelled 3 6 Touch the marker labelled 4...
Страница 15: ...0 59 using the number pad To delete a number touch the Back button Touch Enter The updated time and date settings are displayed swiftly Every time you print your results the updated time and date settings will be included in the print out Press Exit to leave the Time date setting window and return to the system setup menu C 3 8 Display Settings Touch the Display button in the system setup menu To ...
Страница 16: ...th DNA RNA absorbance values and protein absorbance values With SpectroArt 200 you can also measure other forms of nucleic acids at your customised wavelength with your customised conversion factor To measure DNA or RNA enter the setup menu to choose dsDNA ssDNA or RNA under sample target Choose whether to enable 320 nm correction or not Exit setup and read the blank When you touch Sample you are ...
Страница 17: ... and calculated concentration will show on the display D 3 Kinetics With the kinetics function you can follow an enzymatic reaction over time for example the build up of a product from a substrate You specify the total time to measure and then at what intervals to measure within this period Enter the setup menu to specify at what wavelength to measure e g the absorbance of the build up product You...
Страница 18: ...er the DNA RNA setup menu by touching the Setup button The default setting is dsDNA which has a conversion factor of 50 000 µg ml Touch the Sample Target button once or twice to measure ssDNA or RNA respectively The conversion factor changes automatically For customised settings touch the Sample Target button until the nucleic acid window displays custom To change conversion factor touch the Set V...
Страница 19: ... to launch the protein menu Enter the protein setup menu by touching the Setup button The default setting is Bradford at 595 nm To change assay touch the Method button once for Lowry at 750 nm twice for BCA at 562 nm three times for UV protein at 280 nm or four times for UV 320 nm For a customised assay touch the Method button five times Then touch the Change W length button and key in your desire...
Страница 20: ...ur standard curve the lowest concentration using the number pad To delete number touch the Back button Touch Enter Remove the zero sample and insert the sample containing the lowest protein concentration of your standard curve To read the first concentration in the standard curve touch OK If you choose you read the sample more than once repeat the procedure for the designated number of times Repea...
Страница 21: ... be saved When loading a standard curve ensure that the appropriate settings Bradford at 595 nm Lowry at 750 nm BCA at 562 nm UV protein at 280 nm UV 320 nm or customised assay are set before loading the standard curve and measuring the unknown sample To load a standard curve touch S L Touch Load and key in the name for the curve and please be advised some buttons needed to be pressed more than on...
Страница 22: ...h with the X axis showing time and the Y axis showing ABS To get a printed copy of the absorbance values at each interval touch Print D 7 4 OD600 Touch the OD600 button in the main menu to launch the OD600 menu Touch the Setup button to enter the OD600 setup menu Touch the Convert Factor button and key in the conversion factor suitable for the bacteria you are about to measure using the number pad...
Страница 23: ...n Touch Enter to return to the wavelength setup menu To remove a wavelength touch the Remove button and key in the number of the wavelength you wish to remove 200 800 nm To delete number touch the Back button Touch Enter to return to the wavelength setup menu When the wavelength s you wish to measure at are displayed in the wavelength setup menu touch Exit Touch the Blank button Insert a blank sam...
Страница 24: ... values to scan by touching the Y Range button Enter the minimum absorbance value using the number pad To delete number touch the Back button Touch Enter to key in the maximum absorbance value using the number pad To delete number touch the Back button Touch Enter to return to the scan setup menu When the settings you have decided are displayed in the scan setup menu touch Exit Touch the Blank but...
Страница 25: ...cuvettes using a dilute alkaline solution such as 0 1 M NaOH and a dilute acid such as 0 1 M HCl Rinse cuvettes several times with distilled water Always use clean cuvettes before taking absorbance measurements Rigorous cleaning may be after using difficult samples In such cases follow the manufacturer s instruction and use a suitable detergent to clean the cuvettes  Changing Thermal Printer Pape...
Страница 26: ...t fold or crease leading edge of paper to allow for enough space to feed leading edge of the paper into slot opening in paper storage area as shown in the picture below Wind any excess paper back on roll and drop new paper roll with shaft into grooves in paper storage area as shown Let go and lock the side printer latch ...
Страница 27: ...SpectroArt 200 V1 0 24 Close the printer paper compartment lid  Tearing Paper When tearing paper off print outs always tear paper forward against serrated edge of printer outlet as shown ...
Страница 28: ...10 mm lightpath quartz black wall 2pcs 1211002 Semi micro cuvette 1 4 ml 10 mm lightpath quartz black wall 2pcs 1211003 Micro cuvette 700 µl 10 mm lightpath quartz black wall 2pcs 1211004 Micro cuvette 100 µl 10 mm lightpath quartz black wall 2pcs 1211005 Hellma TrayCell Fiber Optic Ultra Micro Cell 5µ 68 5 mm black wall 1 pcs 1211006 Disposable cuvette 3 5 ml 100 pcs 1211007 Didymium filter stand...
Страница 29: ...03070 Wealtec Corp 1885 Meadowvale Way Sparks NV 89431 Tel 1 775 351 2066 Fax 1 775 351 2077 http www wealtec com E mail support wealtec com ...
