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3.1.3 APCI mode

APCI mode, an option for the mass spectrometer, produces singly charged protonated or
deprotonated molecules for a broad range of nonvolatile analytes.

The APCI interface consists of the ESI/APCI/ESCi enclosure fitted with a corona pin and an APCI
probe adapter. Mobile phase from the LC column enters the probe, where it is pneumatically
converted to an aerosol, rapidly heated, and vaporized or gasified at the probe tip.

Figure 3–1: APCI mode

APCI probe

Corona pin

Sample cone

Hot gas from the APCI probe passes between the sample cone and the corona pin, which is
typically operated with a discharge current of 5 µA. Mobile phase molecules rapidly react with
ions generated by the corona discharge to produce stable reagent ions. Analyte molecules
introduced into the mobile phase react with the reagent ions at atmospheric pressure and
typically become protonated (in the positive ion mode) or deprotonated (in the negative ion
mode). The sample and reagent ions then pass through the sample cone and into the mass
spectrometer.

3.1.4 Configuring for ESI/ESCi/APCI modes

To operate in ESI, ESCi or APCI mode, you must fit the correct probe adapter and install a probe
assembly.

January 10, 2022, 715006200 Ver. 01 (previously released as Rev. A)

Page 48

Содержание Xevo TQ-S cronos

Страница 1: ...Waters Xevo TQ S cronos Overview and Maintenance Guide 715006200 Version 01 previously released as Rev A Copyright Waters Corporation 2022 All rights reserved...

Страница 2: ...plete and accurate at the time of publication In no event shall Waters Corporation be liable for incidental or consequential damages in connection with or arising from its use For the most recent revi...

Страница 3: ...rganization invites you to report any errors that you encounter in this document or to suggest ideas for otherwise improving it Help us better understand what you expect from our documentation so that...

Страница 4: ...ervices 34 Maple Street Milford MA 01757 USA Additional resources Waters provides the following additional resources to ensure your continued success with our products Knowledge base Obtain quick answ...

Страница 5: ...ents including vials plates filters column selectivity charts and more Intended use of the Xevo TQ S cronos Waters designed the Xevo TQ S cronos for use as a research tool to accurately reproducibly a...

Страница 6: ...peration Canada spectrum management emissions notice This class A digital product apparatus complies with Canadian ICES 001 Cet appareil num rique de la classe A est conforme la norme NMB 001 ISM clas...

Страница 7: ...cturer Waters Corporation 34 Maple Street Milford MA 01757 USA Safety considerations Some reagents and samples used with Waters instruments and devices can pose chemical biological or radiological haz...

Страница 8: ...dian safety requirements Environmentally friendly use period China RoHS indicates the number of years from the date of manufacture until the product or components within the product are likely to be d...

Страница 9: ...the documentation for important information about the hazard and the appropriate measures to prevent and control the hazard Electrical power safety notice Do not position the device so that it is diff...

Страница 10: ...aters recommends that you perform a hazard analysis assuming a maximum leak into the laboratory atmosphere of 10 LC eluate Warning To avoid exposure to toxic substances and biohazards from O ring leak...

Страница 11: ...inuously through the source A gas fail device is installed to interrupt the flow of LC solvent should the nitrogen supply fail The nitrogen supply pressure does not fall below 400 kPa 4 bar 58 psi dur...

Страница 12: ...avoid personal contamination with biologically hazardous toxic and corrosive materials wear chemical resistant powder free gloves when performing this procedure Warning To avoid puncture injuries han...

Страница 13: ...rea in accordance with laboratory procedures for contaminated vessels and sharps To avoid contamination by carcinogens toxic substances or biohazards you must wear chemical resistant gloves when handl...

Страница 14: ...ety advisories Consult the Safety advisories appendix in this publication for a comprehensive list of warning advisories and notices January 10 2022 715006200 Ver 01 previously released as Rev A Page...

Страница 15: ...ation ISM group 1 class A vi EMC emissions vii EMC grounding requirements vii Legal manufacturer vii Safety considerations vii Applicable symbols vii Safety hazard symbol notice ix Electrical power sa...

Страница 16: ...s 32 1 7 1 Product daughter ion spectrum 33 1 7 2 Precursor parent ion spectrum 33 1 7 3 MRM mode 34 1 7 4 PICS mode 34 1 7 5 RADAR mode 34 1 7 6 Constant neutral loss mode 35 1 8 Sample inlet 36 1 9...

Страница 17: ...for ESI ESCi APCI modes 48 3 1 5 Installing the probe adapter 51 3 1 6 Installing the probe assembly 54 3 1 7 Removing and refitting the probe inlet fitting 60 3 1 8 Connecting the probe assembly to a...

Страница 18: ...4 12 5 Fitting the sampling cone assembly to the source 95 4 13 Cleaning the ion block assembly 96 4 13 1 Removing the ion block assembly from the source assembly 96 4 13 2 Disassembling the source io...

Страница 19: ...y probe and source enclosure seals 130 4 21 2 Fitting the new source enclosure and probe adjuster assembly probe seals 132 4 22 Replacing the air filter 133 4 23 Replacing the UniSpray probe assembly...

Страница 20: ...the workstation 164 B 7 1 Connecting the workstation to the power source 165 B 8 Connecting Ethernet cables systems with ACQUITY LC 165 B 9 I O signal connectors 166 B 9 1 Making I O signal connectio...

Страница 21: ...lti mode electrospray ionization atmospheric pressure chemical ionization combined electrospray ionization and atmospheric pressure chemical ionization ESI APCI ESCi Note ESI supports both the standar...

Страница 22: ...p of the system for use in routine analytical applications waters_connect software includes a dedicated health system that provides immediate feedback and suggestions for resolving common issues The f...

Страница 23: ...S cronos UPLC MS system includes an ACQUITY UPLC ACQUITY UPLC H Class or ACQUITY UPLC I Class and the Waters Xevo TQ S cronos fitted with the ESI APCI ESCi source Note ACQUITY system options can vary...

Страница 24: ...ary solvent manager Sample manager Column heater active UPLC detectors Solvent tray ACQUITY UPLC column Software to control the system ACQUITY Premier Binary solvent manager or quaternary solvent mana...

Страница 25: ...l information see ACQUITY UPLC System Operator s Guide 71500082502 ACQUITY UPLC H Class System Guide 715005049 ACQUITY UPLC I Class System Guide 715003736 ACQUITY Premier System Guide 715006884 ACQUIT...

Страница 26: ...Software and data system You can use either of the following software to control the mass spectrometer MassLynx v4 2 waters_connect The software enables these major operations Configuring the system...

Страница 27: ...a waters_connect applications including Acquisition Method Editor Sample Submission MS Quan LC MS Toolkit UNIFI System Console and Administration are included with the software See the waters_connect...

Страница 28: ...l dedicated high performance APCI interface is available APCI produces singly charged protonated or deprotonated molecules for a broad range of nonvolatile analytes The APCI interface consists of the...

Страница 29: ...or instrument setup and calibration Use 1 5 mL low volume vials sold separately to infuse smaller volumes See Preparing the fluidics system Page 43 The reservoir bottles can also deliver sample throug...

Страница 30: ...about operation of the instrument s solvent delivery system 1 5 Ion optics The mass spectrometer s ion optics operate as follows 1 Samples from the LC or instrument fluidics system are introduced at a...

Страница 31: ...le Conversion dynode Phosphor disk Photomultiplier Stepwave ion guide 1 Probe 1 6 MS operating modes The following table shows the MS operating modes Table 1 2 MS operating modes Operating mode MS1 Co...

Страница 32: ...or MRM analyses derive from the chromatogram plot specified mass intensity SIR or specified transition MRM versus time 1 7 MS MS operating modes The following table shows the MS MS operating modes Ta...

Страница 33: ...ical applications You typically use product ion spectrum for the following applications Method development for MRM screening studies Identifying product ions for use in MRM transitions Optimizing CID...

Страница 34: ...masses MS2 Static at product mass 1 7 3 1 Typical application You typically use MRM mode to quantify known analytes in complex samples forensic or toxicology studies for example screening for target...

Страница 35: ...ss mode Constant neutral loss mode detects the loss of a specific neutral fragment or functional group from an unspecified precursor or precursors The scans of MS1 and MS2 are synchronized When MS1 tr...

Страница 36: ...or Maintenance Instructions 71500082506 for complete details 1 10 Vacuum system An external roughing pump and an internal split flow turbomolecular pump combine to create the source vacuum The turbomo...

Страница 37: ...Event inputs and outputs Shielded Ethernet Waste bottle electrical connection Roughing pump control Source vacuum Collision cell gas inlet Nitrogen inlet Turbo vacuum Source vent Power January 10 202...

Страница 38: ...dix Materials of construction and compatible solvents Page 171 of this guide for mass spectrometer solvent information Appendix C of the ACQUITY UPLC System Operator s Guide 71500082502 for solvent co...

Страница 39: ...ing MassLynx software 1 Power on the workstation and log in 2 Press the power switches on the top left hand side of the ACQUITY instruments and then the power switch on the top right hand side of the...

Страница 40: ...ult When the mass spectrometer is in good operating condition IntelliStart software displays Ready in the Instrument Console Tip If clicking Resolve fails to put the instrument into Operate mode Intel...

Страница 41: ...tain Maintenance Vacuum Pump Instrument 9 Wait for the vacuum to reach the required level Note You can monitor the vacuum status internal pressures and turbo pump speed on the Maintenance pane 10 To p...

Страница 42: ...rate LED indications 2 6 Tuning and calibration information You must tune and if necessary calibrate the instrument prior to use You can perform these tasks using IntelliStart MassLynx or waters_conne...

Страница 43: ...n its front ledge Instead place the bottles in the bottle tray which serves as secondary containment in the event of spills 2 8 1 Installing the reservoir bottles Use standard reservoir bottles 15 mL...

Страница 44: ...To avoid laceration injuries caused by the shattering of fragile low volume glass vials take care when installing them and never use force 3 Screw each low volume vial into the adapter 4 For each low...

Страница 45: ...or similar object into the reset button aperture to operate the reset button Figure 2 2 Rebooting the mass spectrometer Reset button aperture 3 Remove the PEEK tubing from the reset button aperture 4...

Страница 46: ...ain power supply Notice To avoid losing data use the following procedure only in an emergency To reboot the mass spectrometer follow the procedure in the section Rebooting the mass spectrometer To shu...

Страница 47: ...CI modes are all configured using a standard source enclosure 3 1 1 ESI mode To operate in ESI mode you must fit the ESI probe adapter to the source enclosure and install a probe assembly The ESI prob...

Страница 48: ...etween the sample cone and the corona pin which is typically operated with a discharge current of 5 A Mobile phase molecules rapidly react with ions generated by the corona discharge to produce stable...

Страница 49: ...Probe adapter types ESI probe adapter APCI probe adapter ESI identification label APCI probe heater ESI probe tip APCI identification label January 10 2022 715006200 Ver 01 previously released as Rev...

Страница 50: ...SI ESCi APCI modes Mode Probe adapter Install corona pin ESI ESI No ESCi ESI Yes APCI APCI Yes For more information on using each mode see the Xevo TQ S cronos system online Help The following section...

Страница 51: ...e buttons Required materials Chemical resistant powder free gloves To install the probe adapter Warning To avoid personal contamination with biologically hazardous or toxic compounds wear clean chemic...

Страница 52: ...e in the source enclosure s probe adjuster assembly ensuring that the probe location dowel aligns with the location hole in the probe adjuster assembly Figure 3 6 Probe location dowel Probe location d...

Страница 53: ...ng ring clockwise to secure the probe adapter in place Figure 3 9 Probe adapter mounted on the source enclosure Probe adapter cap Probe adapter locking ring Probe adjuster assembly Source enclosure Hi...

Страница 54: ...o verify the probe assembly type Ensure that the probe adapter is installed on the source with the probe adapter cap removed See Installing the probe adapter Page 51 To minimize delays and dispersion...

Страница 55: ...damaging the probe assembly take care when inserting the capillary into the probe adapter Do not use force Warning To avoid harmless static like electric shock ensure that the mass spectrometer is in...

Страница 56: ...y until you hear a click Figure 3 13 Probe assembly fitted to the probe adapter Probe adapter fitting Tip The probe adapter fitting varies in size depending on the probe assembly type If you cannot fi...

Страница 57: ...robe cap aperture from the underside Probe cap aperture from the top Tip The probe assembly tubing can pass through the aperture only when the ball bearing is located in the recess at the bottom and d...

Страница 58: ...onto the probe adapter until it clicks Tips Do not squeeze the probe adapter cap release buttons when fitting the probe adapter cap Ensure that the probe adapter cap is correctly seated and that both...

Страница 59: ...rate the high voltage supply to the probe turns on and the instrument performs a pressure test To prevent gas escaping audibly through the probe adapter ensure that the instrument is not in Operate mo...

Страница 60: ...bly to a UPC system Page 62 3 1 7 Removing and refitting the probe inlet fitting To feed the probe assembly through narrow channels you might need to remove the probe inlet fitting and then refit it o...

Страница 61: ...ting spring retainer in place while the probe inlet fitting is removed to prevent it from sliding along the probe assembly Figure 3 18 Removing the probe inlet fitting Fitting spring retainer Fitting...

Страница 62: ...use the supplied UPC fitting to connect the probe assembly to a UPC system Do not use the PEEK inlet fitting Required materials Chemical resistant powder free gloves 1 4 inch open end wrench To connec...

Страница 63: ...l you encounter resistance Figure 3 21 Connecting the probe assembly to the ISM Compression nut backing ring and ferrule To ISM restrictor port 3 Slide the compression nut backing ring and ferrule int...

Страница 64: ...04382 Replacing the ESI probe tip or gasket see Replacing the ESI probe tip and gasket Page 122 Tip You can replace the probe assembly without removing the ESI probe tip You can remove the probe adapt...

Страница 65: ...g To avoid personal contamination with biologically hazardous toxic and corrosive materials wear chemical resistant powder free gloves when performing this procedure Warning To avoid harmless static l...

Страница 66: ...g that the corona pin is securely mounted and that its tip aligns with the sample cone orifice Figure 3 25 Corona pin Corona pin 5 Close the source enclosure 6 Look through the source window and use t...

Страница 67: ...Warning To avoid puncture wounds take great care while working with the source enclosure open if an ESI probe is fitted the ESI probe tip is sharp 2 Pull the source enclosure release located at the bo...

Страница 68: ...ray standard source front Probe PEEK fitting Probe assembly Vertical probe adjuster Horizontal probe adjuster Source enclosure door release handle January 10 2022 715006200 Ver 01 previously released...

Страница 69: ...e also Topics about maintaining the source components Replacing the UniSpray probe assembly Page 135 Maintaining the impactor pin Page 138 3 2 1 Installing the UniSpray source 3 2 1 1 Installing the U...

Страница 70: ...Warning To avoid harmless static like electric shock ensure that the instrument is in Standby mode before beginning this procedure Warning To avoid burn injuries exercise care when handling the compo...

Страница 71: ...juster cable socket yellow on the mass spectrometer 8 Screw the probe assembly s PEEK fitting into the appropriate fluidics port according to your instrument until it is finger tight 9 Close source in...

Страница 72: ...ce 1 To prepare the instrument for working on the source stop solvent flow ensure that the instrument is in Standby mode and stop desolvation gas flow Warning To avoid burn injuries exercise care when...

Страница 73: ...from the instrument source interface panel 5 Disconnect the impactor pin high voltage cable from the instrument source interface panel 6 Return the cable fittings to their storage slots on the source...

Страница 74: ...level Weekly See Maintaining the rotary backing pump s oil Page 85 Clean the source components When source components are visibly fouled the background or high peak contaminants are unacceptably high...

Страница 75: ...iSpray mode When the impactor pin is corroded or black or the sensitivity decreases to unacceptable levels See Maintaining the UniSpray impactor pin Page 138 Replace the source assembly seals Annually...

Страница 76: ...bol To touch external areas marked with the symbol first put the instrument in Standby mode Warning To avoid burn injuries take great care while working with the probe and source these components can...

Страница 77: ...e desolvation gas flow 5 Lift the visor on the front of the instrument so that it is clear of all the source components and probe 4 4 2 Using waters_connect software to prepare the instrument for oper...

Страница 78: ...instrument for working on the source see Preparing the instrument for working on the source Page 76 Warning To avoid burn injuries exercise care when handling the components of the source enclosure h...

Страница 79: ...s To fit the source enclosure to the instrument Warning To avoid personal contamination with biologically hazardous toxic and corrosive materials wear chemical resistant powder free gloves when perfor...

Страница 80: ...dures Required materials Chemical resistant powder free gloves 4 6 1 Closing the source isolation valve To close the source isolation valve before starting a maintenance procedure Warning To avoid per...

Страница 81: ...tamination with biologically hazardous toxic and corrosive materials wear chemical resistant powder free gloves when performing this procedure Warning To avoid puncture wounds take great care while wo...

Страница 82: ...s and seals You must remove O rings or seals from instrument components when performing certain maintenance procedures 4 7 1 O ring removal kit Note The O ring removal kit 700005054 is available to or...

Страница 83: ...logically hazardous toxic and corrosive materials dispose of all waste materials according to local environmental regulations 4 8 Cleaning the instrument case Notice To avoid abrading the surfaces of...

Страница 84: ...ttle To laboratory exhaust port Valve control cable from instrument Cap Bottle support Nitrogen exhaust trap bottle From instrument exhaust connection One way valve January 10 2022 715006200 Ver 01 pr...

Страница 85: ...cordance with local environmental regulations 5 Fit and tighten the nitrogen exhaust trap bottle to the cap 6 Secure the nitrogen exhaust trap bottle in the upright position 7 Close the source enclosu...

Страница 86: ...mbol Warning To avoid slip injuries inspect the pump for oil leaks To add oil to the backing pump 1 Power off the instrument using the power button in the top left hand corner of the front panel 2 Wai...

Страница 87: ...inlet on the pump s top side Tip If you encounter difficulty remove the separator 10 Operate the pump briefly 11 Remove the oil drain plug and drain the flushing oil into a suitable container 12 If ne...

Страница 88: ...eaning the ion guide assembly Page 111 4 12 Cleaning the sampling cone assembly You can remove the sampling cone assembly comprising the sample cone O ring and cone gas nozzle for cleaning without ven...

Страница 89: ...Closing the source isolation valve Page 80 2 Grasp the cone gas nozzle handle and use it to rotate the sampling cone assembly 90 degrees moving the handle from the vertical position to the horizontal...

Страница 90: ...g cone assembly Warning To avoid personal contamination with biologically hazardous toxic and corrosive materials wear chemical resistant powder free gloves when performing this procedure 1 Retrieve t...

Страница 91: ...llar Notice To avoid damaging the sampling cone which is fragile do not place it on its tip Always place it on its flanged base 4 Rotate and lift the tool and collar to remove the sample cone from the...

Страница 92: ...tions 6 If the O ring shows signs of deterioration or damage dispose of it in accordance with local environmental regulations 4 12 3 Cleaning the sample cone and cone gas nozzle Required materials Che...

Страница 93: ...sembly is removable although the cone gas nozzle can be cleaned without detaching the handle Tip If the components are obviously contaminated use 45 45 10 methanol water formic acid 3 Place the vessel...

Страница 94: ...spose of the component according to local environmental regulations and obtain a new one before reassembling the sampling cone assembly 4 12 4 Assembling the sampling cone assembly Required materials...

Страница 95: ...erials wear chemical resistant powder free gloves when performing this procedure Warning To avoid puncture wounds take great care while working with the source enclosure open if one or both of these c...

Страница 96: ...source isolation valve Page 81 5 Close the source enclosure 4 13 Cleaning the ion block assembly Clean the ion block assembly if cleaning the sample cone cone gas nozzle and extraction cone fails to...

Страница 97: ...tes before proceeding Warning To avoid puncture wounds take great care while working with the source enclosure open if one or both of these conditions apply An ESI probe is fitted the probe s tip is s...

Страница 98: ...ined 2 5 mm hex wrench and cone extraction tool O ring removal kit To disassemble the ion block assembly Warning To avoid personal contamination with biologically hazardous toxic and corrosive materia...

Страница 99: ...pling cone assembly out of the ion block assembly 4 Use the combined 2 5 mm hex wrench and cone extraction tool to loosen the two captive screws securing the ion block cover plate Important To ensure...

Страница 100: ...te 6 Grasp the isolation valve and pull it out of the ion block Figure 4 20 Removing the isolation valve from the ion block Isolation valve O ring 7 Use the O ring removal kit to carefully remove the...

Страница 101: ...erminal block securing screw Figure 4 21 Loosening the PEEK terminal block securing screw Heater cartridge assembly wires PEEK terminal block securing screw Notice To avoid damaging the heater cartrid...

Страница 102: ...moval kit to carefully remove the cover seal from the ion block see also Removing O rings and seals Page 82 Figure 4 23 Removing the cover seal from the ion block Cover seal Cone gas O ring 12 Use the...

Страница 103: ...taining HPLC grade or better 1 1 methanol water Large beaker To clean the ion block components Warning To avoid personal contamination with biologically hazardous toxic and corrosive materials wear ch...

Страница 104: ...ert oil free gas Warning To avoid spreading contamination with biologically hazardous toxic and corrosive materials dispose of all waste materials according to local environmental regulations 6 Inspec...

Страница 105: ...fits the isolation valve 7 Fit the isolation valve to the ion block assembly so that it is in the closed position 8 Fit the ion block cover plate to the ion block assembly and then use the combined 2...

Страница 106: ...e instrument Page 79 5 Close the source enclosure 6 Connect the power cords for the mass spectrometer and the roughing pump to the main power source 4 14 Cleaning the ion guide assembly Clean the ion...

Страница 107: ...moving the pumping block assembly and ion guide assembly Ion guide assembly Housing Pumping block assembly Securing screws 4 Notice To avoid damaging the ion guide assembly when removing it from or fi...

Страница 108: ...1 Use the 3 mm hex wrench to unscrew and remove the 2 screws securing the differential aperture and ion guide assembly boards to the pumping block assembly Figure 4 25 Removing the ion guide assembly...

Страница 109: ...ade screwdriver O ring removal kit Warning To avoid personal contamination with biologically hazardous toxic and corrosive materials wear chemical resistant powder free gloves when performing this pro...

Страница 110: ...ntamination with biologically hazardous toxic and corrosive materials dispose of all waste materials according to local environmental regulations 4 14 4 Cleaning the differential aperture Notice To pr...

Страница 111: ...perture a second time 7 Fill the cleaning vessel with isopropyl alcohol until the differential aperture is immersed completely 8 Carefully remove the differential aperture from its cleaning vessel and...

Страница 112: ...sed damage to the ion guide assembly ensure that the bottom of the assembly is not in contact with the bottom of the glass vessel 3 Bend the opposite end of the tube into a hook shape that allows you...

Страница 113: ...6 Fitting the differential aperture and the differential aperture support onto the ion guide assembly Required materials Chemical resistant powder free gloves 2 5 mm hex wrench Flat blade screwdriver...

Страница 114: ...e pumping block assembly Required materials Chemical resistant powder free gloves 3 mm hex wrench Warning To avoid personal contamination with biologically hazardous toxic or corrosive materials and t...

Страница 115: ...ent Required materials Chemical resistant powder free gloves 3 mm hex wrench Warning To avoid personal contamination with biologically hazardous toxic or corrosive materials and to avoid spreading con...

Страница 116: ...ng block assembly Securing screws 4 2 Use the 3 mm hex wrench to thread and tighten the 4 screws into the pumping block assembly and housing to secure the pump block assembly to the instrument 3 Fit t...

Страница 117: ...performing this procedure 1 Remove the ion block assembly from the instrument see Removing the ion block assembly from the source assembly Page 96 2 Ensure that the isolation valve is closed Figure 4...

Страница 118: ...ptive PEEK terminal block securing screw Heater cartridge assembly wires PEEK terminal block securing screw Notice To avoid damaging the heater cartridge assembly wires do not bend or twist them when...

Страница 119: ...EK terminal block 8 Disconnect the heater cartridge wires from the PEEK terminal block 9 Use the needle nose pliers to gently grasp the heat shrink tubing on the heater cartridge assembly and slide th...

Страница 120: ...tighten the captive PEEK terminal block securing screw 16 Fit the ion block cover plate to the ion block assembly and then use the combined 2 5 mm hex wrench and cone extraction tool to tighten the t...

Страница 121: ...cap off the probe adapter sliding it over the probe assembly Tip Removing the probe adapter cap disconnects the high voltage supply and the gas flow to the probe and stops the source nebulizer and de...

Страница 122: ...l regulations 5 To install a new probe assembly see Installing the probe assembly Page 54 4 17 Replacing the ESI probe tip and gasket Replace the ESI probe tip if a blockage occurs in the internal met...

Страница 123: ...avoid puncture injuries handle sample needles syringes fused silica lines and borosilicate tips with extreme care 1 Remove the ESI probe adapter from the source see Removing the probe adapter Page 64...

Страница 124: ...nd gasket Required materials Chemical resistant powder free gloves 10 mm open end wrench 7 mm open end wrench New metal gasket To fit the ESI probe tip and gasket Warning To avoid personal contaminati...

Страница 125: ...rometer s online Help 4 18 Cleaning the APCI probe tip Clean the APCI probe tip when you detect buffer buildup on the probe tip or when the signal intensity weakens See the mass spectrometer s online...

Страница 126: ...s can be hot Warning To avoid personal contamination with biologically hazardous toxic and corrosive materials wear chemical resistant powder free gloves when performing this procedure 1 Remove the pr...

Страница 127: ...f the probe heater in accordance with local environmental regulations 4 19 2 Fitting the new APCI probe heater Required materials Chemical resistant powder free gloves APCI probe heater To fit the new...

Страница 128: ...oid damaging the probe heater s electrical connections do not twist the heater when removing it from or refitting it to the probe adapter 2 Fit the probe adapter to the instrument see Installing the p...

Страница 129: ...cture injuries handle sample needles syringes fused silica lines and borosilicate tips with extreme care 1 Remove the corona pin from the source see Removing the corona pin from the source Page 67 2 R...

Страница 130: ...tion gas seal Source enclosure seal Nebulizer gas seal Desolvation gas seal 4 21 1 Removing the probe adjuster assembly probe and source enclosure seals Required materials Chemical resistant powder fr...

Страница 131: ...gas seal Probe adjuster assembly probe seal 3 Use the O ring removal kit to carefully remove the following seals from the source enclosure Source enclosure seal Nebulizer gas seal Desolvation gas seal...

Страница 132: ...tamination to uncontaminated surfaces wear clean chemical resistant powder free gloves when performing this procedure 1 Ensure that all the grooves for seals are free from dirt and debris Tip If conta...

Страница 133: ...e 79 4 22 Replacing the air filter You must replace the air filter annually Required materials New air filter To replace the air filter 1 Lift the instrument s visor so the source probe is fully expos...

Страница 134: ...er grill Probe cable 4 Open the air filter grill by pulling the tab at the top of the grill toward you Figure 4 47 Replacing the air filter Air filter tab Air filter grill January 10 2022 715006200 Ve...

Страница 135: ...23 1 Removing the UniSpray probe assembly Required materials Chemical resistant powder free gloves Warning To avoid personal contamination with biologically hazardous toxic and corrosive materials we...

Страница 136: ...ll a replacement UniSpray probe assembly see Installing the UniSpray probe assembly Page 136 4 23 2 Installing the UniSpray probe assembly 4 23 2 1 Installing the UniSpray probe assembly Required mate...

Страница 137: ...hot components have sufficiently cooled before you handle them 2 Remove the existing probe assembly from the source see Removing the UniSpray probe assembly Page 135 3 Carefully insert the probe assem...

Страница 138: ...nt powder free gloves UniSpray impactor pin Warning To avoid personal contamination with biologically hazardous toxic and corrosive materials wear chemical resistant powder free gloves when performing...

Страница 139: ...uired materials Chemical resistant powder free gloves Lapping film HPLC grade or better methanol Lint free tissue Impactor pin Warning To avoid personal contamination with toxic materials wear clean c...

Страница 140: ...ircuitry Circuits can remain live even when one fuse has blown Warning To avoid electrical fire ensure that replacement fuses comply with the ratings affixed to the rear panel of the module If one or...

Страница 141: ...damage resulting from the failure of individuals to comply with any safety precaution when installing repairing or operating any of its instruments or devices The following symbols warn of risks that...

Страница 142: ...from bursting nonmetallic tubing heed these precautions when working in the vicinity of such tubing when it is pressurized Wear eye protection Extinguish all nearby flames Do not use tubing that is or...

Страница 143: ...uiring the use of flammable solvents A 1 1 4 Biohazard warning The following warning applies to Waters instruments and devices that can process biologically hazardous materials Biologically hazardous...

Страница 144: ...ated statement alert you to such risk Notice To avoid damaging the case of the instrument or device do not clean it with abrasives or solvents A 3 Bottles Prohibited symbol The Bottles Prohibited symb...

Страница 145: ...l quipement Warnung Jedwede nderungen oder Modifikationen an dem Ger t ohne die ausdr ckliche Genehmigung der f r die ordnungsgem e Funktionst chtigkeit verantwortlichen Personen kann zum Entzug der...

Страница 146: ...tubes s v rement d form s ou endommag s Evitez d utiliser des tubes non m talliques avec du t trahydrofurane THF ou de l acide sulfurique ou nitrique concentr Sachez que le chlorure de m thyl ne et le...

Страница 147: ...ione di rottura dei tubi stessi Advertencia se recomienda precauci n cuando se trabaje con tubos de pol mero sometidos a presi n El usuario deber protegerse siempre los ojos cuando trabaje cerca de tu...

Страница 148: ...ection assur e par le mat riel risque d tre d fectueuses Warnung Der Benutzer wird darauf aufmerksam gemacht dass bei unsachgem er Verwendung des Ger tes die eingebauten Sicherheitseinrichtungen unter...

Страница 149: ...bles par d autres du type et de la puissance indiqu s sur le panneau proximit du couvercle de la boite fusible de l instrument Warnung Zum Schutz gegen Feuer die Sicherungen nur mit Sicherungen ersetz...

Страница 150: ...ersetzen deren Typ und Nennwert im Abschnitt Sicherungen ersetzen des Kapitels Wartungsverfahren angegeben sind Avvertenza per garantire protezione contro gli incendi sostituire i fusibili con altri...

Страница 151: ...t Indicates that the device or assembly is susceptible to damage from electrostatic discharge ESD A 8 Handling symbols The following handling symbols and their associated statements can appear on labe...

Страница 152: ...Symbol Description Fragile Use no hooks Upper limit of temperature Lower limit of temperature Temperature limitation January 10 2022 715006200 Ver 01 previously released as Rev A Page 152...

Страница 153: ...ice before moving the instrument If you must transport the instrument or remove it from service contact Waters Technical Service for recommended cleaning flushing and packaging procedures B 1 External...

Страница 154: ...Waste bottle electrical connection Roughing pump control Source vacuum Collision cell gas inlet argon Nitrogen inlet Turbo vacuum Source vent Power January 10 2022 715006200 Ver 01 previously released...

Страница 155: ...mp Exhaust port Vacuum hose assembly Power cable connector Required tools and materials Chemical resistant powder free gloves 7 mm nut driver 8 mm hex wrench Utility knife NW25 clamp included in the i...

Страница 156: ...peration of the backing pump it must be installed within one degree of horizontal Notice The area where the backing pump is located must have an ambient temperature of 15 to 40 C 59 to 104 F Note To e...

Страница 157: ...instrument s rear panel Note To avoid gas leaks use the sharp knife to cut the PVC exhaust tubing squarely 5 Use a hose clamp to connect a length of 19 mm clear PVC exhaust tubing to the pump exhaust...

Страница 158: ...ry backing pump 1 Connect the pump control cable from the pump to the backing pump connector on the instrument s rear panel 2 Connect the rotary backing pump power cord to the mains power source B 3 C...

Страница 159: ...0 cm 1 5 inch to 2 inch length of 6 mm PTFE tubing from the other end of the tubing Requirement Cut the tubing squarely 5 Connect one end of the 3 8 cm to 5 0 cm PTFE tubing to the nitrogen supply in...

Страница 160: ...inch stainless steel tube to the collision cell gas inlet on the rear of the instrument see the figure in Connecting to the nitrogen gas supply Page 158 2 Use the wrench to tighten the 1 8 inch Swage...

Страница 161: ...sed by routing exhaust lines incorrectly 1 Locate the exhaust trap bottle in an accessible area below the instrument 2 Cut a length of 12 mm tubing long enough to connect the instrument to the exhaust...

Страница 162: ...ust line To laboratory exhaust port 12 mm Valve control cable from instrument Cap Bottle support Nitrogen exhaust trap bottle From instrument waste 12 mm One way valve January 10 2022 715006200 Ver 01...

Страница 163: ...free gloves when performing this procedure 1 Place a suitable waste container below the mass spectrometer Notice To avoid distorting the drip tray or causing the drain cup to leak restrain the drain...

Страница 164: ...nnecting to the workstation Warning To avoid injury from electrical shock or fire and damage to the equipment follow these guidelines Do not expose the workstation or ancillary equipment to dripping o...

Страница 165: ...ons 1 Select the appropriate power cord for your location 2 Connect the female end of the power cord to the power port on the rear panel of the workstation B 8 Connecting Ethernet cables systems with...

Страница 166: ...nd UV RI and fluorescence detector signal outputs for LC MS systems The electrical connections on the rear panel of this mass spectrometer are all SELV The instrument s rear panel includes two removab...

Страница 167: ...nt in connections Signal connections Description Analog Out Used for analog chart output functionality The output voltage range is 0 to 1 V The resolution of the voltage output is 12 bits Stop Flow Ou...

Страница 168: ...um 30 V 0 5 A 10 W Requirement To meet the regulatory requirements of immunity from external electrical disturbances install connection covers over the signal connectors To make signal connections 1 R...

Страница 169: ...mp and shield Clamp Shield Connection cover 5 Insert the connector with the signal cable into the connection cover and position the clamp over the cable leads Tighten the clamp into place with the sec...

Страница 170: ...type power cord in the United States and HAR type or better in Europe The main power cord must be replaced with one of adequate rating For information regarding what cord to use in other countries co...

Страница 171: ...appear in the following table can be exposed to solvent You must evaluate the safety issues if the solvents used in your application differ from the solvents typically used with these items See Solven...

Страница 172: ...repare mobile phases for reverse phase LC MS API Water Methanol Acetonitrile Formic acid 0 1 Acetic acid 0 1 Ammonium acetate 50 mM Ammonium formate 50 mM Trifluoroacetic acid TFA 0 2 These solvents a...

Страница 173: ...n find this document on www waters com by clicking Support Support Documents and Downloads D 2 The selector valve The selector valve is located behind the visor on the right hand side of the instrumen...

Страница 174: ...ed in the solvent in the wash reservoir Figure D 1 Plumbing schematic P W R B A S LC LC column Selector valve Waste Wash Reservoir B Reservoir A Pump Xevo TQ S cronos probe D 4 Tubing and connection s...

Страница 175: ...elow for the correct specifications Table D 2 Replacement tubing specifications Port ID in Color PEEK tubing Length m R A and B 0 020 Orange 0 65 W 0 005 Red 0 2 S 0 004 Black 0 4 LC 0 004 Black As re...

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