5.
In the console, select the solvent manager, and then click
Control
>
Prime seal wash
>
Yes
to begin the seal wash priming process.
6.
Slowly withdraw the syringe plunger, to pull solvent through the system.
7.
When the seal-wash solution begins to flow into the syringe with relatively few air bubbles,
click
Control
>
Prime seal wash
, to stop the priming process.
8.
Disconnect the tubing from the syringe assembly, and reconnect it to the fitting on the drip
tray.
2.7 Priming the solvent manager
Priming prepares a new system or solvent manager for use and for a change in reservoirs or
solvents. It also prepares a system for restarting after it has been idle for more than four hours.
During priming, the vent valve moves to the vent position, ensuring minimal back pressure and
directing solvent flow to waste. The flow rate during priming is 4 mL/min.
Tip:
If you are priming a dry solvent manager, using a syringe shortens the time required for
priming.
Recommendation:
Ensure that solvents, which combine at the inlet manifold, are fully
miscible.
Notice:
To prevent salts from precipitating in the system, introduce an intermediate
solvent, such as water, when changing from buffers to high-organic-content solvents.
Be sure to consult the solvent miscibility tables in the Solvent Considerations section of
the system guide.
Ensure that the solvent reservoirs contain sufficient solvent for adequate priming and that the
waste container can hold all the used solvent. For example, at 4 mL/min, priming for 5 minutes
uses about 20 mL of each solvent.
Warning:
To avoid spills, empty the waste container at regular intervals.
Requirement:
Prime all solvent lines with solvent, to ensure that the degasser functions
properly.
2.7.1 Priming a dry solvent manager via the console
To prime a dry solvent manager via the console:
1.
Open the solvent manager’s front door.
2.
Locate the solvent vent tubing.
August 8, 2016, 715005050 Rev. C
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