Waters 2707, Руководство оператора

Страница: 28 / 100

1-18 Understanding Operating Principles
Partial loop injections
Partial loop injections yield no sample loss and, like partial loop needle overfill
injections, maximum accuracy. However, their reproducibility specification is
slightly lower. In the standard configuration, peak area RSD is < 1% for
injection volumes > 10 µL.
Recommendation:
Because wash solvent injects with the sample in
partial loop mode, consider the effect on the chromatography. In general
for reverse phase chromatography, match the wash solvent mixture to
the initial gradient conditions.
This is the partial loop injection sequence:
1. The injection valve moves to the Inject position, and the sample needle
moves to the Transport position.
Wash station
Buffer tubing
Syringe
Needle
Sample loop
INJECT
Column
Pump
Sample