Waters 1500-Series, Руководство оператора

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Startup and initial preparation 4-3
Priming, purging, and equilibrating procedures are fully described later in
this chapter.
Eluent guidelines
When you prepare a 1500-series pump for operation, follow these guidelines:
• Use methanol to prime a new pump, or one that has been stored for an
extended period.
• Ensure that the solvents are totally miscible at all compositional
combinations to prevent precipitation, check valve fouling, and other
related problems.
• Use an intermediate solvent when you change between eluents that are
not miscible.
• Use only HPLC-grade eluents, to obtain accurate, reproducible
chromatography.
• To prevent air from being drawn into the pump, keep the eluent level in
the reservoir at least four inches (10 cm) higher than the inlet manifold.
Do not place eluent reservoirs on top of a 1500-series pump.
• Use only filtered, degassed eluents.
• After use with a buffer, purge the pump with HPLC-grade water.
• When possible, use eluents of similar viscosity when you run a gradient.
• Consider column chemistry. If the column can be damaged by an
intermediate or new eluent, remove it from the system, and substitute a
union. Refer to the care and use guide for your column.
Changing eluent
Adding fresh eluent
Changing columns
System has been idle for some
time
Pump preparation recommendations (Continued)
Status Prime Purge Equilibrate