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SD-OCT Base Unit

Chapter 4: System Operation

Rev E, November 7, 2018

Page 35

4.5. Example

Images

Frequency Domain OCT can be used for a wide range of real-time monitoring applications in biological and

clinical fields as well as in manufacturing and materials science. This technology is ideal for in-line industrial

imaging applications ranging from laminated packaging films to 3D visualization of mechanical parts.

Skin Imaging

SD-OCT provides real-time high resolution surface and sub-surface imaging of human skin, making this system

ideal for many dermal and sub-dermal applications, including burn-depth monitoring, wound healing, and cancer

detection.

Figure 39 B-Scan of a Nailfold

Figure 40 B-Scan of a Fingertip

Figure 41 B-Scan of Skin and Nailfold. Top – Intensity-Based OCT image, Bottom – Optic Axis Polarization-

Sensitive OCT image

Содержание CAL110

Страница 1: ...SD OCT Base Unit Spectral Domain OCT System Base Units Telesto Ganymede and Callisto Series User Manual...

Страница 2: ...Original User Manual not translated...

Страница 3: ...Optical Coherence Tomography 19 Nomenclature in OCT imaging 20 3 2 OCT Base Unit Components 22 Base Unit 22 PC with Graphical User Interface 22 SDK 23 Imaging Scanner Accessory 24 OCT STAND Accessory...

Страница 4: ...s 52 7 2 Declaration of Conformity GAN6x0 Series Base Units 53 7 3 Declaration of Conformity TEL2x0 Series Base Units 54 7 4 Declaration of Conformity TEL2x0PS Series Base Units 55 7 5 Declaration of...

Страница 5: ...t specified the base units of the Telesto PS Series are incorporated in the Telesto Series ATTENTION Check the supply voltage of the system before plugging in the computer Make sure the included power...

Страница 6: ...sed within the range of three meters of this unit since the electromagnetic field intensity may exceed the maximum allowed disturbance values according to IEC 61000 6 1 2005 The safety of any system i...

Страница 7: ...perature Handle all connectors both electrical and optical with care Do not use unnecessary force as this may damage the connectors Handle the optical fiber with care Mechanical stress can decrease pe...

Страница 8: ...plugging it into a receptacle 3 Always cover the fiber end that is not in use with a fiber cap or dust protection cover The cleaning procedure sucess could be visualized using a fiber inspection scope...

Страница 9: ...sert the tip of the cleaner into the bulkhead connector Push the case to start the cleaning process a click indicates that the cleaning is complete Cleaning Fiber Connectors Figure 4 Cleaning Fiber Co...

Страница 10: ...essories and Customization The OCT base unit can easily be adapted for custom interfaces To achieve the listed specifications however this system should only be used with the accessories that Thorlabs...

Страница 11: ...lesto PS Series base units a fiber cable is permanently installed D Auxiliary Fiber Connection to Imaging Scanner FC APC Not in Telesto Series and some Ganymede Series base units E Probe Connection Po...

Страница 12: ...pin configuration 2 3 Optical Interface to Probe The base unit can incorporate one or two FC APC fiber interfaces depending on the internal fiber architecture see also chapter 3 1 2 Base units with an...

Страница 13: ...stem to reach room temperature before attempting to operate Operate this system on a flat dry and stable surface only 1 Install the PC monitor mouse and keyboard according to the documentation provide...

Страница 14: ...tween the base unit and the SMB tee connector which is plugged into the PC Use the short cable to connect the second framegrabber with the open port of the SMB tee connector Figure 7 Connection of SMB...

Страница 15: ...he imaging scanner Align the red dot of the plug to the alignment mark of the electric connection port of the scanner e g OCTG Figure 8 Plugging the Electric Connector into the Scanner Figure 9 Electr...

Страница 16: ...and clean it according to chapter 1 2 2 Figure 10 Fiber Connection to the Imaging Scanner A Slide the fiber tip into the center bore of the fiber connection B C The fiber needs to be oriented in rota...

Страница 17: ...9 Attach the electric connection cable to the base unit Align the red dot upwards facing the alignment mark in the base unit Figure 11 Installing the Electric Connection Cable at the Base Unit Push t...

Страница 18: ...from the fiber end and from the FC APC fiber connection at the base unit Inspect the fiber and clean it according to chapter 1 2 2 Figure 12 Fiber Connection to the Base Unit A Slide the fiber tip int...

Страница 19: ...ting the trigger signal The location of this connector is shown in Figure 14 This option is not available in Callisto and Telesto PS Series base units ATTENTION To make use of the external trigger the...

Страница 20: ...d OCT systems and SS OCT for the time encoded systems In both types of systems light is split by a fiber coupler into the sample and reference arms of an interferometer setup Back reflected light attr...

Страница 21: ...the image depth could be doubled The signal width has two limits One limit for the signal width is given by the spectral distribution of the light source For a fully used Gaussian shaped light source...

Страница 22: ...meter where the unique phase delay for each wavelength is detected To gain access to this back scattered light it is necessary to insert a splitting device into the optical path In Thorlabs SD OCT sys...

Страница 23: ...f the reflected beam needs to be rotated by 45 when interfering with the sample beam To implement this a quarter wave plate QWP is used in the reference arm The polarization axis is rotated by 22 5 to...

Страница 24: ...scribed before the SD OCT engine creates a depth profile from the interference of photons reflected off layers of the sample with photons reflected in the reference arm This depth profile is referred...

Страница 25: ...the volume or by 3D rendering Please refer to the OCT Software Manual for all features available Figure 21 Rendered Volumetric Data Set Figure 22 En Face View or C Scan When displaying a plane with b...

Страница 26: ...lication is returned through the fiber optic coupler or circulator which directs the light to a spectrometer as described in Figure 16 or Figure 17 Other components in the base unit include a high spe...

Страница 27: ...ogrammer can Define either a standard scanner provided by Thorlabs or create a software representation of a custom built device Define simple or complex scan patterns Acquire simple A Scans B Scans or...

Страница 28: ...ntegration of the interferometer eliminates the problems associated with chromatic or polarization mode dispersion that are introduced by differences between individual fibers in the sample and refere...

Страница 29: ...SD OCT Base Unit Chapter 3 Description Rev E November 7 2018 Page 25 Figure 26 OCTP PS User Customizable PS OCT Scanner Figure 27 OCTH Handheld Scanner...

Страница 30: ...26 MTN012389 D02 OCT STAND Accessory The Thorlabs OCTG and OCTP scanners can be mounted to an OCT STAND For this OCT STAND the rotation and translation stage OCT XYR1 is available Figure 28 OCT STAND...

Страница 31: ...D OCT Base Unit Chapter 3 Description Rev E November 7 2018 Page 27 Figure 30 OCT XYR1 Sample Rotation Stage For further details on the OCT STAND and the OCT XYR1 please refer to the Thorlabs web site...

Страница 32: ...will illuminate and stabilized with respect to temperature and the related performance parameters The warm up takes approximately 10 seconds to complete 4 2 Basic Adjustments When receiving a complet...

Страница 33: ...n acquisition When significantly misadjusted you will get an incorrect OCT image or no image of your sample irrespective of the focus position Pull out the reference length adjustment knob from the lo...

Страница 34: ...is out of focus and the right is in focus The focus can usually be identified by one of more of the following features Sharp thin features in lateral direction Autocorrelation terms see chapter 5 3 H...

Страница 35: ...mples with a small axial focus length an objective lens with a high numerical aperture can be chosen This will grant a small lateral and axial focus hence allowing a more in depth analysis of the samp...

Страница 36: ...scan Figure 36A After finding the focus autocorrelation terms will appear in the upper part of the image even without any reflecting surface in the field of view of the B scan When these artifact sig...

Страница 37: ...might be helpful to flip the whole image of the sample see chapter 5 6 Note that you can use the software to flip the image back to its original position however this is purely cosmetic and does not...

Страница 38: ...th Matte Surface Partially Covered with Clear Adhesive Tape Figure 38 shows a B scan of a plastic substrate with a matte surface The right part of the surface was covered with a strip of adhesive tape...

Страница 39: ...ging applications ranging from laminated packaging films to 3D visualization of mechanical parts Skin Imaging SD OCT provides real time high resolution surface and sub surface imaging of human skin ma...

Страница 40: ...cal material science applications Such systems are ideal for monitoring surface topography and layered structures Figure 42 B Scan of a Semi Transparent Molded Plastic Cap Figure 43 B Scan of a Lamina...

Страница 41: ...tem Operation Rev E November 7 2018 Page 37 Biological Imaging Figure 45 B Scan of a Section of a Grape Figure 46 B Scan of carious tooth Top Intensity Based OCT image Bottom Degree Of Polarization Un...

Страница 42: ...sponse For example a sinusoidal optical signal is interpreted as partially rectangular Consequently additional harmonic frequencies of the root signal appear Figure 47 High Surface Reflection Causing...

Страница 43: ...ell above noise level and well below saturation In the OCT software a colored bar indicates if the reference intensity is set properly For adjustment of the reference intensity please refer to chapter...

Страница 44: ...ween interference created in respect to the reference arm and interference created within the sample Figure 50 shows the B scan of a sample laminated foils Figure 50 Autocorrelation from a Surface Ref...

Страница 45: ...n deeper in the image than physically present Figure 52 Schematic of a Setup to Show the Influence of Multiple Scattering Multiple scattering is intense in paper For illustration of this artifact a se...

Страница 46: ...930nm the maximum detectable speed v in the direction of the A scan axis is 290 m s When the direction of the movement occurs at an angle with respect to the A scan axis faster speeds can be imaged F...

Страница 47: ...or longer from the beam splitter to the sample compared to the reference arm length When adjusting the reference length to be too long the image appears flipped Figure 55 Right Orientation Up Referenc...

Страница 48: ...formation one can only see information from regions in the sample where photons are transmitted to and allowed back into the sampling aperture Reflections strong scattering and absorption lead to shad...

Страница 49: ...distance from the beam splitter to the scattering or reflecting object These path lengths are optical path lengths calculated from the physical path length multiplied by the group refractive index In...

Страница 50: ...modulation or envelope of the wave propagates through space This velocity usually is different from the speed of the phases of the single wavelengths This velocity is calculated by using the group re...

Страница 51: ...Depth With Refractive Media In the image the incoming beam from above is scanned over a structure made of two different materials named one d1 ng1 and two d2 ng2 stacked on a flat surface red line The...

Страница 52: ...of the materials assuming the underlying surface to be flat and horizontal in the image In the OCT image on the right the physical thicknesses i d optical path lengths i gi d n as well as the resultin...

Страница 53: ...al path and the displayed OCT image makes it difficult to perform an inverse ray tracing because all the diffractive interfaces need to be determined in 3D Even this determination needs to be undertak...

Страница 54: ...connected Connect fiber patch cable Fiber tip is dirty Clean fiber tip Scanner not connected Connect Scanner Focus is out of imaging area Adjust reference length readjust focus Reference intensity to...

Страница 55: ...be connected to the unit Slide open the cover of the fuse holder located at the rear panel of the base unit as shown in Figure 23 Remove the broken fuse s and install the appropriate replacement fuse...

Страница 56: ...SD OCT Base Unit Chapter 7 Certifications and Compliance Page 52 MTN012389 D02 Chapter 7 Certifications and Compliance 7 1 Declaration of Conformity GAN2x0 Series Base Units...

Страница 57: ...SD OCT Base Unit Chapter 7 Certifications and Compliance Rev E November 7 2018 Page 53 7 2 Declaration of Conformity GAN6x0 Series Base Units...

Страница 58: ...SD OCT Base Unit Chapter 7 Certifications and Compliance Page 54 MTN012389 D02 7 3 Declaration of Conformity TEL2x0 Series Base Units...

Страница 59: ...SD OCT Base Unit Chapter 7 Certifications and Compliance Rev E November 7 2018 Page 55 7 4 Declaration of Conformity TEL2x0PS Series Base Units...

Страница 60: ...SD OCT Base Unit Chapter 7 Certifications and Compliance Page 56 MTN012389 D02 7 5 Declaration of Conformity TEL3x0 Series Base Units...

Страница 61: ...SD OCT Base Unit Chapter 7 Certifications and Compliance Rev E November 7 2018 Page 57 7 6 Declaration of Conformity CAL1x0 Series Base Units...

Страница 62: ...ormed 8 3 Warranty Exclusions The stated warranty does not apply to products which are a specials modifications or customized items including custom patch cables meeting the specifications you provide...

Страница 63: ...ution Air Water typical 12 9 0 m 5 5 4 2 m 12 9 0 m 5 5 4 2 m Lateral Resolution at Focus with OCT Scan Lens Kit 20 0 m OCT LK4 13 0 m OCT LK3 20 0 m OCT LK4 13 0 m OCT LK3 Sensitivity 96 111 dB 76 5...

Страница 64: ...ength 930 nm Axial Scan Rate 1 2 KHz Maximum Imaging Depth Air Water typical 1 7 mm 1 3 mm Axial Resolution Air Water typical 7 0 m 5 3 m Lateral Resolution at Focus with OCT Scan Lens Kit 8 0 m OCT L...

Страница 65: ...SD OCT Base Unit Chapter 10 Mechanical Drawings Rev E November 7 2018 Page 61 Chapter 10 Mechanical Drawings Figure 64 Base Unit Dimensions...

Страница 66: ...roducts such as Pure OEM products that means assemblies to be built into a unit by the user e g OEM laser driver cards Components Mechanics and optics Left over parts of units disassembled by the user...

Страница 67: ...USA Canada and South America Thorlabs Inc sales thorlabs com techsupport thorlabs com Europe Thorlabs GmbH europe thorlabs com France Thorlabs SAS sales fr thorlabs com Japan Thorlabs Japan Inc sales...

Страница 68: ...M0009 510 1071 D www thorlabs com...

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