ThermoFisher Scientific Vanquish VC-D50, Руководство по эксплуатации

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8 • Troubleshooting
Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual Page 163
4. Allow the sensor to adjust to the ambient temperature for a few
minutes.
5. If leakage is no longer reported, you can resume operation.
See also
2 Removing the Flow Cell ( } page 136)
2
Installing the Flow Cell (
}
page 139)
8.3.2 Additional Device Operating Issues
This section provides additional issues that may arise during operation of
the Vanquish device. Locate the table for the type of symptom you have,
find the possible cause, and use the description of the solution to help
you solve your problem quickly.
Also check the Chromeleon Audit Trail for a related message if an
operating problem occurs. The message may provide additional
information.
Note that this section provides information on symptoms and causes
directly related to the Vanquish device. For information about
troubleshooting for the Vanquish system, refer to the
Vanquish System
Operating Manual .
TIP If you are unable to resolve a problem following the instructions
given here or if you experience problems that are not covered in this
section, contact Thermo Fisher Scientific Technical Support for
assistance.
Symptom Possible Cause Remedial Action
Peak tailing Fluorescence detector
flow cell inlet and outlet
exchanged Verify that the flow cell inlet and outlet in a
fluorescence detector are connected correctly.
Negative peaks Absorption/fluorescence
of analyte lower than of
mobile phase Select different UV or fluorescence detection
wavelengths.
Use a mobile phase with less background
absorption/ fluorescence.
Dissolve the sample in mobile phase.
Fluorescence of the
substance or eluent is
quenched by other
components Consider using the negative peaks for
quantification.
Markers in fluorescence
signal The Sensitivity was
automatically reduced Select a lower Sensitivity for your application. The
sample audit trail informs to which level the
Sensitivity was reduced to.