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Appendix

Page 184

Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11)

Operating Manual

11.1.5 Manual Release History

Revision

Covering

2.0

VC‑D11-A-01; VC‑D12-A-01; VF‑D11‑A‑01

1.0

VF‑D11‑A‑01

The instructions were prepared in English (original instructions). Other
language versions are translations based on the English original
instructions.

Содержание Vanquish VC-D11

Страница 1: ...thermoscientific Vanquish Diode Array Multiple Wavelength Detectors VC D11 VC D12 VF D11 Operating Manual 4820 8302 EN Revision 2 0 December 2019...

Страница 2: ...ded as is The contents of this manual are subject to being changed without notice in future revisions Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or e...

Страница 3: ...organization For contact information go to Contact Us on http www thermofisher com Technical Assistance For technical support for HPLC products contact your local Thermo Fisher Scientific support org...

Страница 4: ...Contacting Us Page 4 Diode Array Multiple Wavelength Detectors VC D11 VC D12 VF D11 Operating Manual...

Страница 5: ...l 18 2 1 3 Safety Symbols on the Device 19 2 1 4 Rating Plate 19 2 2 Intended Use 20 2 3 Safety Precautions 21 2 3 1 General Safety Information 21 2 3 2 Qualification of the Personnel 22 2 3 3 Persona...

Страница 6: ...ting the Device 57 5 5 3 Connecting the Power Cord 58 5 6 Installing a VIS Lamp Tungsten Lamp Optional 59 5 7 Installing a Flow Cell 62 5 8 Setting Up the Flow Connections 67 5 8 1 General Information...

Страница 7: ...and Service 114 7 2 Safety Guidelines for Maintenance and Service 115 7 2 1 General 115 7 2 2 Flow Cells 117 7 3 General Rules for Maintenance and Service 118 7 4 Routine and Preventive Maintenance 1...

Страница 8: ...54 7 14 4 Setting Up the Slide In Module 156 8 Troubleshooting 157 8 1 General Information about Troubleshooting 158 8 2 Messages 160 8 3 Checking the Flow Cell 166 8 4 Resolving Liquid Leaks 167 9 Sp...

Страница 9: ...Contents Diode Array Multiple Wavelength Detectors VC D11 VC D12 VF D11 Operating Manual Page 9 11 2 UV Cutoff Wavelengths of Solvents 185 Index 187...

Страница 10: ...Contents Page 10 Diode Array Multiple Wavelength Detectors VC D11 VC D12 VF D11 Operating Manual...

Страница 11: ...h Detectors VC D11 VC D12 VF D11 Operating Manual Page 11 1 Using this Manual This chapter provides information about this manual the conventions used throughout the manual and the reference documenta...

Страница 12: ...followed properly Note the following The device configuration may vary therefore not all descriptions necessarily apply to your particular device If some detail applies to only one model or variant t...

Страница 13: ...y a particular section or procedure appear in the section or procedure to which they apply They appear different from the main flow of text Safety messages are often preceded by an alert symbol and or...

Страница 14: ...All Programs Thermo Chromeleon 7 Services Manager Start Instrument Controller References and Messages References to additional documentation appear italicized Messages that appear on the screen are i...

Страница 15: ...e Reader or Adobe Acrobat is required Software Documentation Additional software documentation includes the following Chromeleon Help and documents The Chromeleon Help provides extensive information a...

Страница 16: ...VC D12 VF D11 Operating Manual TIP The Chromeleon Help and documents are included in the software shipment Third Party Documentation Refer also to the user documentation provided by the manufacturers...

Страница 17: ...Diode Array Multiple Wavelength Detectors VC D11 VC D12 VF D11 Operating Manual Page 17 2 Safety This chapter provides general and specific safety information and informs about the intended use of th...

Страница 18: ...re doing CAUTION Indicates a hazardous situation that if not avoided could result in minor or moderate injury WARNING Indicates a hazardous situation that if not avoided could result in serious injury...

Страница 19: ...g operation Do not touch these surfaces while they are heated up Indicates that the UV radiation produced by the deuterium lamp in the device may be harmful to eyes and skin Do not look directly into...

Страница 20: ...d Vanquish system are intended to be used as General Laboratory Equipment GLE They are not intended for use in diagnostic procedures Laboratory Practice Thermo Fisher Scientific recommends that the la...

Страница 21: ...additional components options and peripherals specifically authorized and qualified for the device by Thermo Fisher Scientific Perform only the procedures that are described in this operating manual a...

Страница 22: ...he module and system General Operation The device is designed to be operated only by trained and qualified personnel in a laboratory environment All users must know the hazards presented by the device...

Страница 23: ...mage to the Device High voltages are present inside the device that could cause an electric shock or damage to the device Do not make any changes to the electrical or grounding connections If you susp...

Страница 24: ...ctice Reduce the volume of substances to the minimum volume required for sample analysis Do not operate the device in a potentially flammable environment Avoid accumulation of harmful substances Make...

Страница 25: ...nce of flammable gases or fumes CAUTION Escape of Hazardous Substances from PEEK Capillaries In the Vanquish system capillaries made of PEEK may be used Swelling or attack by acids can cause PEEK capi...

Страница 26: ...ity This effect is particularly present with insulating capillaries and non conductive solvents for example pure acetonitrile Discharge of electrostatic energy might lead to sparking which could const...

Страница 27: ...only Use only solvents and additives that are compatible with all parts in the flow path TIP In a Vanquish Core system normal phase NP compatible solvents and additives may be used if the VC pumps an...

Страница 28: ...for flushing the system disconnect the detector from the system Mobile phases containing ammonium hydroxide In rare cases a shortened lifetime of reversed phase UHMW PE piston seals has been observed...

Страница 29: ...of the other modules in the Vanquish system refer to the Specifications chapter in the Operating Manual for the modules Follow any specific recommendations presented in other sections of this manual R...

Страница 30: ...nual Changes that you make to the device may void compliance with one or more of these EMC and safety standards Changes to the device include replacing a part or adding components options or periphera...

Страница 31: ...3 Device Overview Diode Array Multiple Wavelength Detectors VC D11 VC D12 VF D11 Operating Manual Page 31 3 Device Overview This chapter introduces you to the device and the main components...

Страница 32: ...etector optional feature for the VC detectors Data Collection Characteristic VF D11 VC D11 VC D12 Data collection rate Up to 250 Hz Up to 125 Hz Up to 125 Hz Number of signal channels at individual wa...

Страница 33: ...atically moves into the following positions Optimization Features Purpose Open For data acquisition Closed dark For performing autozero Holmium oxide filter For validation of wavelength accuracy Optio...

Страница 34: ...follows the Beer Lambert law This means that the response of the detector is proportional to the Concentration of the analyte Path length of the light in the sample The following image shows the opti...

Страница 35: ...hat the beams pass through the flow cell 5 After exiting the flow cell the light is focused by a lens 6 and if the motorized filter paddle 7 is in its open position the light passes through the entran...

Страница 36: ...ated directly behind the front doors Figure 2 Interior view with flow cell installed No Description 1 Cooling air intake 2 Keypad with status indicators 3 Lamp house cover 4 Flow cell after installati...

Страница 37: ...l Label One or more flow cell labels are present on the flow cell which contain information such as flow cell type part number and serial number Flow Cell Identification Chip An identification ID chip...

Страница 38: ...ure before the mobile phase enters the optical flow path within the flow cell Note that the volume of the heat exchanger and inlet capillary influences the retention times and peak widths Flow Cell Ty...

Страница 39: ...um lamp for ultraviolet UV wavelength detection Tungsten lamp for visible VIS and near infrared NIR wavelength detection standard for the VF detector optional for the VC detectors The lamp house conta...

Страница 40: ...ce for liquid leaks from the flow connections The liquid is collected in the leak tray and guided to the drain port From the drain port the liquid is discharged to waste through the drain system of th...

Страница 41: ...trol and automated sample analysis with the Chromeleon software refer to the Vanquish System Operating Manual Details on control and operation of the device are available in the Chromeleon Help TIP Th...

Страница 42: ...3 Device Overview Page 42 Diode Array Multiple Wavelength Detectors VC D11 VC D12 VF D11 Operating Manual...

Страница 43: ...king Diode Array Multiple Wavelength Detectors VC D11 VC D12 VF D11 Operating Manual Page 43 4 Unpacking This chapter provides information for unpacking the device and informs you about the scope of d...

Страница 44: ...surance will compensate for the damage only if reported immediately Unpacking the Device CAUTION Heavy Load Bulky Device The device is too heavy or bulky for one person alone to handle safely To avoid...

Страница 45: ...No Component 1 Carrying handles 2 Attachment screw one on each carrying handle 4 Place the device on a stable surface 5 If applicable Remove any additional packing material Leave any protective films...

Страница 46: ...ng off the carrying handle from the left rail TIP Keep the shipping container the carrying handles with the attachment screws and all packing material These items will be needed if the device is trans...

Страница 47: ...D11 Operating Manual Page 47 4 2 Scope of Delivery The following items are included in the delivery Detector Ship Kit Operating manual Power cord For information on contents of the ship kit or reorde...

Страница 48: ...4 Unpacking Page 48 Diode Array Multiple Wavelength Detectors VC D11 VC D12 VF D11 Operating Manual...

Страница 49: ...ctors VC D11 VC D12 VF D11 Operating Manual Page 49 5 Installation This chapter specifies the requirements for the installation site and describes how to set up install and configure the device in the...

Страница 50: ...rsons A team effort is in particular required when lifting the device into the system stack or when removing it Use the carrying handles that were shipped with the device to move or transport the devi...

Страница 51: ...erefore follow the order for installing the system modules as described in the Vanquish System Operating Manual 1 Pay attention to the safety guidelines and observe all site requirements See Safety Gu...

Страница 52: ...s NOTICE If the device is operated with another data system refer to the documentation for the software that you are using and or perform the qualification manually The Instruments Installation Qualif...

Страница 53: ...anquish system as stated in the Vanquish System Operating Manual For specifications see Specifications page 169 and the Specifications sections in the Operating Manuals for the other modules in the Va...

Страница 54: ...power outlet that has a protective ground connection In case of emergency it must be possible to reach the power cord easily at any time to disconnect the device from the power line WARNING Electric S...

Страница 55: ...ual Page 55 5 4 Accessing the Interior Components To access the interior components in the device open the front doors To allow easy access from the front the user accessible components and flow conne...

Страница 56: ...bles 5 5 1 System Arrangement The device is part of the Vanquish system The system modules are typically arranged in a system stack with the arrangement depending on the system configuration Figure 7...

Страница 57: ...ddress 2 Main power switch on off control 3 Fuse holder 4 Power inlet connector 5 System Interlink port Allows power on off control for the device from the Vanquish system base and device communicatio...

Страница 58: ...vice in the system as required by the system configuration For details refer to the Vanquish System Operating Manual 2 Connect the required interface cables to the device For information about how to...

Страница 59: ...6 Installing a VIS Lamp Tungsten Lamp Optional Follow the instructions below to install the optional VIS lamp Parts required VIS lamp Preparations 1 Turn off the device Follow these steps Figure 9 VIS...

Страница 60: ...t protection plate for later use 5 Align the new lamp with the locating pin When the lamp is in the correct position push the lamp gently into the lamp house The lamp socket must be in a level positio...

Страница 61: ...detector in the Instrument Configuration Manager b Select Properties on the menu c On the Detector page enable the VIS Lamp check box 12 After installing a new lamp increased noise and strong baseline...

Страница 62: ...f the flow cell are sensitive to contamination and scratches Do not immerse the optical ports of the flow cell Particulate matter dust and debris can lead to contamination and clogging of the flow cel...

Страница 63: ...d do not reach into the flow cell opening while no flow cell or diagnostic cell is installed and the UV lamp is turned on 3 Hold the flow cell with the flow cell body to the left firmly squeeze the ha...

Страница 64: ...t only Press the locating pin of the connection unit into the slot provided on the interior front panel Figure 12 Installing a flow cell with connection unit No Description 1 Connection unit 2 Connect...

Страница 65: ...is tilted by the force of kinked capillaries If the flow cell is seated correctly Arrange the capillaries in such a way that the capillaries do not exert any force on the flow cell and the flow cell...

Страница 66: ...sure that the capillaries run through the slot in the flow cell cover Figure 14 Fixing the flow cell cover here flow cell with connection unit 9 Fasten the two screws of the flow cell cover hand tigh...

Страница 67: ...cautionary statements presented in Safety Precautions page 21 NOTICE Particulate matter from other system modules and components can deposit in the flow cell and clog it Before you connect the flow ce...

Страница 68: ...ow cell or waste line Improperly set up flow connections can lead to leaks on the flow cell Do not invert the flow cell inlet and outlet Connecting dirty inlet capillaries can lead to leakages and can...

Страница 69: ...es of the Vanquish system are guided through either the tubing chase in the devices or the guide holes or capillary clips of the devices Tubing Chase Tubing Guide Tubing Bracket To guide certain tubes...

Страница 70: ...g chase Guide Holes and Capillary Clips Guide holes and capillary clips are provided at specific positions on the system modules Route flow connections from one module to the next module in the Vanqui...

Страница 71: ...panel push the plug with slit in the required recess of the partition panel if not present yet 2 To secure the plug with slit insert the nose into the opening of the partition panel Figure 18 Securin...

Страница 72: ...ing capillary fittings Viper nanoViper Standard 1 16 HPLC fittings using ferrules PEEK SST or finger tight fittings PEEK This section provides information about how to connect and handle capillaries f...

Страница 73: ...use tools other than the knurl that comes with the capillary To avoid damage to the capillary or connection tighten and loosen the Viper capillaries only when the system pressure is down to zero Figur...

Страница 74: ...Guidelines page 67 Parts required Inlet capillary Detector waste line For instructions on connecting the waste line follow the steps in Connecting the Detector Waste Line page 76 Tools required For t...

Страница 75: ...the Inlet Capillary Preparations See Flow Connections to the Flow Cell page 74 Follow these steps Figure 22 Connecting the inlet capillary from the column compartment example 1 Route the inlet capilla...

Страница 76: ...ug with slit such as an insulated inlet capillary use the rotating plug 3 Connect the inlet capillary to the flow cell inlet port IN 5 8 5 2 Connecting the Detector Waste Line Preparations 1 See Flow...

Страница 77: ...waste line over the entire flow path Make sure that no bending kink pinching or squeezing of the waste line is present at any point in the flow path Assembling the waste line if the tubing slips out...

Страница 78: ...Liquid Leaks to Waste Leaking liquids of the device are collected in the leak tray where they flow off through the chase on the right side of the leak tray to the drain system For information about h...

Страница 79: ...ating system can detect the device To turn on the power to the device follow these steps 1 Check that the power button on the front left of the Vanquish system base system power button is pressed in I...

Страница 80: ...assumes that the chromatography software is already installed on the data system computer and a valid license is available For more information about setting up the Vanquish system in the software ref...

Страница 81: ...iode Array Multiple Wavelength Detectors VC D11 VC D12 VF D11 Operating Manual Page 81 6 Operation This chapter describes the elements for device control provides information for routine operation and...

Страница 82: ...ed If this is not the case refer to the instructions in Installation page 49 For a basic description of instrument control and automated sample analysis with the Chromeleon software refer to the Vanqu...

Страница 83: ...n Touching hot parts might cause burns Allow hot surfaces to cool down before you touch them NOTICE Pay attention also to the following guidelines When operating the chromatography system always set t...

Страница 84: ...device and the STATUS LED on the keypad provide a quick visual check of the operational status of the device 6 3 1 Keypad The keypad inside the device allows you to perform certain functions directly...

Страница 85: ...V lamp is turned off Green flashing The UV lamp ignites Green The UV lamp is turned on VIS The VIS button allows you to turn the VIS lamp on and off directly from the detector To turn on the VIS lamp...

Страница 86: ...is equilibrating At least one lamp is igniting Green The device is equilibrated but no data acquisition is running A flow cell or diagnostic cell has been detected and at least one lamp is turned on...

Страница 87: ...rve the following All modules in the Vanquish system that are connected to the system base via system interlink cables are turned on or off simultaneously when the system power button is pressed When...

Страница 88: ...was stored it is filled with isopropanol Use solvents that are miscible with isopropanol If they are not use an appropriate intermediate solvent To remove the isopropanol from the flow cell Verify th...

Страница 89: ...mpartment thermostatting in the autosampler Flow cell in a fluorescence detector Evaporation tube in a charged aerosol detector Turning on the lamp or lamps in the UV VIS detector Monitoring the pump...

Страница 90: ...ler in the flow path before the flow cell Allow the flow cell to warm up The detector the flow cell and the inlet capillary are sensitive to thermal changes in the environment If the doors are opened...

Страница 91: ...s with high salt content in the flow cell without flow Flush the flow cell regularly when using concentrated salt solutions Monitor the backpressure via the pump pressure Make sure that buffer salts r...

Страница 92: ...growth of algae Additionally follow the guidelines in Short Term Shutdown Interruption of Operation page 108 During long analysis breaks take appropriate measures to protect the flow cell Turn off th...

Страница 93: ...buttons on the keypad of the detector or using Chromeleon see Important Operating Parameters page 95 TIP To ignite the lamps the lamp house cover must be installed If you remove the lamp house cover w...

Страница 94: ...th a flow rate of 0 05 mL min If the detector is not used for a longer time for example over the weekend turn off the UV lamp to save lamp life time and energy VIS Lamp You can turn off the VIS lamp u...

Страница 95: ...to Chromeleon Help and documents General Parameters Parameters Description UV lamp VIS lamp optional Turn on the UV and or VIS lamp before starting an analysis UV_Lamp On Visible_Lamp On This setting...

Страница 96: ...on Instrument Method Wizard the response time and peak width are defined automatically For guidelines on selecting the data collection rate see Selecting the Data Collection Rate page 101 Bandwidth Th...

Страница 97: ...avelength is set To use the Reference Wavelength setting select it separately for each signal channel For further information about the reference wavelength see Selecting the Reference Wavelength and...

Страница 98: ...bled This is the preferred setting Wavelength validation Perform the wavelength validation as required A holmium oxide glass filter is moved into the light path and is used to validate the wavelength...

Страница 99: ...re the solvents absorb strongly for example below 220 nm for methanol and below 210 nm for acetonitrile Ignite the lamps only if required for more information see Guidelines for Use of the Lamps page...

Страница 100: ...data collection rate and the response time the time averaging during data acquisition TIP Except for the slit width parameter you can set the parameters shown in the table below back to factory defaul...

Страница 101: ...rate is too low the start points maxima and end points of peaks will not be determined accurately If the data collection rate is too high data files may need more disk space and post run analyses may...

Страница 102: ...ore averaging of the signal and results in less short term noise However if the selected response time is too long this can result in reduced peak heights and asymmetrical peak shapes If a separation...

Страница 103: ...rform a detection at a wavelength of 195 nm with a bandwidth of 20 nm the photodiode bunching is set to 190 to 200 nm i e the largest possible symmetrical range around the detection wavelength As a re...

Страница 104: ...e wavelengths Higher Lower Baseline noise Higher Lower Usage High concentrations Analytes with narrow absorption bands such as benzene Low concentrations Normal applications Figure 25 Effects of the s...

Страница 105: ...IP The use of a reference wavelength can result in additional interference in the chromatogram Use the reference wavelength carefully and only in special cases In most cases measurement without a refe...

Страница 106: ...y for each signal channel and the 3D field only available for the VC D11 and VF D11 detectors Select a reference bandwidth that is as broad as possible such as 30 100 nm It should also be narrow enoug...

Страница 107: ...behaves in these cases Zero sets the baseline to zero Append appends the baseline to the previous signal Free lets the baseline jump to the current absolute value TIP You can perform the AutoZero com...

Страница 108: ...system modules as required by your system arrangement For your Vanquish detector note the following Detector Type Description Charged aerosol detector Check that sufficient gas is available to contin...

Страница 109: ...rameters for the other system modules are set as required before proceeding 6 8 2 Long Term Shutdown Shutting Down the Device To interrupt operation for a longer period follow the instructions below T...

Страница 110: ...ter shutdown Flush the system for example with methanol 100 acetonitrile should not be used Flush the system with several volumes of methanol and water 50 50 for example 1 0 mL min for 10 minutes with...

Страница 111: ...to zero before you continue the shutdown of the device 8 Disconnect the capillaries from the flow cell inlet and outlet 9 After removing the flow connections protect the flow cell inlet and outlet po...

Страница 112: ...Flush the components in the flow path before the flow cell before you connect the flow cell to the system flow path 3 Connect the inlet capillary to the flow cell inlet and the waste line to the flow...

Страница 113: ...rvice Diode Array Multiple Wavelength Detectors VC D11 VC D12 VF D11 Operating Manual Page 113 7 Maintenance and Service This chapter describes the routine maintenance and the service procedures that...

Страница 114: ...r Scientific for brevity referred to as Thermo Fisher Scientific service personnel The device is designed for easy maintenance and service The user serviceable parts of the device can be accessed from...

Страница 115: ...his manual WARNING Escape of Hazardous Substances from Flow Connections Flow and capillary connections can be filled with substances that can pose health risks Solvent can spray when capillaries burst...

Страница 116: ...CAUTION Hydrostatic Pressure Solvent may spill when you open the flow path This is due to hydrostatic pressure in the system when the solvent reservoirs are located above the pump outlet Before you l...

Страница 117: ...hanical shocks mechanical vibrations or intruding objects can lead to leaks on the flow cell or even destroy it Avoid exposure of the flow cell to mechanical shocks or vibrations Do not let it hit har...

Страница 118: ...use the tools recommended for the procedure Before opening the flow path to replace capillaries in the system turn off the pump flow and wait until the system pressure is down to zero Dirty components...

Страница 119: ...after use with an appropriate solvent that does not contain buffers or salts Monitor the backpressure that the flow cell is exposed to Regularly Inspect the flow connections for damage such as cracks...

Страница 120: ...rooms Avoid open flames or exposure to excessive heat during the cleaning process Wipe the cleaned components thoroughly dry after cleaning Do not operate the device before it is completely dry NOTIC...

Страница 121: ...cation information about the device These functions are called Predictive Performance They allow you to schedule maintenance procedures based on the actual operating and usage conditions of the device...

Страница 122: ...ith an external standard such as a pyrene solution In this case an accuracy of 1 nm can be achieved When A wavelength validation is recommended in the following situations After the flow cell has been...

Страница 123: ...elength validation fails perform a wavelength calibration from Chromeleon see next section To perform a wavelength calibration TIP To ensure optimum performance and wavelength accuracy perform Wavelen...

Страница 124: ...is detector may result in inferior performance detector malfunction or may damage the detector Detector guaranty may be voided Use only lamps supplied or approved by Thermo Fisher Scientific for this...

Страница 125: ...e Array Multiple Wavelength Detectors VC D11 VC D12 VF D11 Operating Manual Page 125 Follow these steps Figure 27 UV lamp No Description 1 UV lamp connector 2 UV lamp 3 UV lamp attachment screw 4 UV l...

Страница 126: ...p may become hot Touching a hot lamp might cause burns Touch the lamp briefly and carefully to find out if it is hot before you remove the lamp If it is hot wait until the lamp has cooled down 4 Remov...

Страница 127: ...ched under the lamp house cover 14 Reinstall the lamp house cover and fasten the screws on the lamp house cover 15 Turn on the device with its main power switch 16 After replacing the lamp increased n...

Страница 128: ...mount of operating hours of the lamp as a preventive measure The lamp intensity is too low for demanding applications The lamp intensity fluctuates causing artifacts in the baseline Parts required VIS...

Страница 129: ...burns Touch the lamp briefly and carefully to find out if it is hot before you remove the lamp If it is hot wait until the lamp has cooled down 4 Push the clip on the lamp connector and disconnect the...

Страница 130: ...be ignited from Chromeleon a Right click the detector in the Instrument Configuration Manager b Select Properties on the menu c On the Detector page enable the VIS Lamp check box 14 After replacing t...

Страница 131: ...e Long Term Shutdown page 109 4 Install the new flow cell to the device see Installing a Flow Cell page 62 7 7 1 Guidelines for Handling Flow Cells NOTICE Flow cells are sensitive to dirt and dust Obs...

Страница 132: ...w cell Preparations 1 Remove buffer salts by flushing the flow cell with a mixture of 50 solvent and 50 water 2 Flush the flow cell with a highly pure solvent that is miscible with isopropanol such as...

Страница 133: ...ow Cell page 134 7 7 2 1 Performing a Manual Injection A sample can also be injected manually using the flushing and injection kit Parts required Flushing and injection kit for flow cells including a...

Страница 134: ...d follow good laboratory practice Refer to the material handling and safety data sheet provided by the vendor Follow these steps You can perform the following procedure by using the optional flushing...

Страница 135: ...cool down before you remove it NOTICE Do not open the tubing connections between the connection unit and the flow cell The tubing connections are installed at the factory 1 Loosen the two screws of t...

Страница 136: ...t reach into the flow cell opening while no flow cell or diagnostic cell is installed and the UV lamp is turned on 7 If the flow cell is to be stored Store the flow cell in its original packaging that...

Страница 137: ...ell Preparations 1 Loosen the two screws of the flow cell cover remove the cover and keep the cover to close the flow cell opening after this procedure 2 Remove the flow cell see Removing the Flow Cel...

Страница 138: ...iagnostic cell is properly installed Check against the positioning marks no 1 in the figure below whether the diagnostic cell is seated correctly When the diagnostic cell is installed correctly the de...

Страница 139: ...ll or diagnostic cell is installed and the UV lamp is turned on 2 Store the diagnostic cell in its packaging 3 If a flow cell is to be installed afterward Install the flow cell to the flow cell openin...

Страница 140: ...from the flow cell through the open leakage drain system of the Vanquish system Never expose the flow cell to excessive backpressure Avoid clogging of the flow cell or waste line When connecting a com...

Страница 141: ...outlet 3 Remove the waste line from the top recess of the partition panel If the waste line is installed in a rotating plug Turn the plug in the top recess to the front and remove the waste line from...

Страница 142: ...gh voltages are present inside the device that could cause an electric shock or damage to the device Turn off the device with its main power switch Disconnect the power cord from both the power source...

Страница 143: ...Page 143 1 Use the screwdriver to remove the fuse holder 2 Replace the two fuses with new fuses of the specified type and current rating Always replace both fuses 3 Reinstall the fuse holder 4 Reconn...

Страница 144: ...Chromeleon software 3 Stop all operations on the Instrument that includes the device 4 Wait until the Instrument is idle Follow these steps 1 Start the Instrument Configuration Manager program 2 Perfo...

Страница 145: ...m to see whether the firmware update was successful or failed If the firmware update failed turn the device off and on again and repeat the firmware update If the firmware update fails repeatedly cont...

Страница 146: ...steps in this section Parts required Replacement door Follow these steps NOTICE To avoid damage to the door hinges be careful when performing the following sequence of steps and do not apply force 1...

Страница 147: ...m the housing and remove the door 3 To install the door align the door with the hinges on the housing Be careful not to clamp tubing or capillaries between the door and the enclosure 4 Insert the hing...

Страница 148: ...in particular required when lifting the device into the system stack or when removing it Use the carrying handles that were shipped with the device to move or transport the device Never move or lift t...

Страница 149: ...tack in the Transporting or Shipping the System section of the Vanquish System Operating Manual or Remove the slide in module from the device enclosure in the system stack see Removing the Slide In Mo...

Страница 150: ...only the original packing material and shipping container If the original shipping container is not available appropriate containers and packing material can be ordered from the Thermo Fisher Scienti...

Страница 151: ...operating manual 2 Install and set up the device in the system stack Follow the instructions on mounting the system stack in the Vanquish System Operating Manual 3 Set up the device a Connect the dev...

Страница 152: ...eam effort of two persons A team effort is in particular required when lifting the device into the system stack or when removing it Tools required Screwdriver Torx T20 Preparations 1 Prepare the devic...

Страница 153: ...Pulling out the slide in module NOTICE The slide in module can fall down when pulling it out of the enclosure too far Pull out the slide in module just far enough so that you can grasp it on both side...

Страница 154: ...is operating manual CAUTION Possible Contamination Hazardous substances may have contaminated the device during operation and may cause personal injury to service personnel Decontaminate all parts of...

Страница 155: ...ubing and capillaries which are present in the tubing chase of the Vanquish system modules into the tubing chase Otherwise you will not be able to insert the slide in module properly into the enclosur...

Страница 156: ...lled properly If the screws move tighten the screws further With a torque wrench the recommended torque is 1 2 Nm 8 Set up and restart the device 7 14 4 Setting Up the Slide In Module After you have r...

Страница 157: ...hooting Diode Array Multiple Wavelength Detectors VC D11 VC D12 VF D11 Operating Manual Page 157 8 Troubleshooting This chapter is a guide to troubleshooting issues that may arise during operation of...

Страница 158: ...he keypad inside provide quick visual feedback on the operational status of the device If the device firmware detects a problem the status indicators are red The problem is reported to the Chromeleon...

Страница 159: ...failure occurred during operation of the module an exception log has been created about the processes during the firmware failure The firmware sends the exception log to the Audit Trail when the modu...

Страница 160: ...ur digit code number When the message appears write down the message code and turn off the module Wait for 5 seconds and turn on the module again If the message appears again contact Technical Support...

Страница 161: ...or Contact Technical Support Update the firmware Code 113 Module malfunction detected The firmware may be defective Update the firmware see Updating the Device Firmware page 144 Code 3022 Unexpected m...

Страница 162: ...sh the flow cell Check whether the flow cell is installed properly Check the flow cell see Checking the Flow Cell page 166 Flush the flow cell with pure HPLC grade water for several minutes with a flo...

Страница 163: ...that the available lamps are on perform a calibration and repeat the validation again Ensure that the flow cell is installed properly Remove and install the flow cell Flush the flow cell with pure LC...

Страница 164: ...vironmental temperature Code 3198 Invalid spectral data Check flow cell Invalid spectral data for absorption calculation Make sure that the mobile phase does not show excessive absorption for the sele...

Страница 165: ...ady in progress The lamp is igniting or shutting down wait for completion Code 3207 Unexpected module behavior Check the flow cell see Checking the Flow Cell page 166 Change and revert the slit width...

Страница 166: ...with a different flow cell The problems are caused by the flow cell that was previously installed If you suspect a contamination or an elevated backpressure perform a flushing procedure with the flow...

Страница 167: ...is visible 2 If the connection seems tight but is still leaking remove the capillary waste line and check for damage 3 If the capillary waste line is damaged replace the respective component 4 If the...

Страница 168: ...8 Troubleshooting Page 168 Diode Array Multiple Wavelength Detectors VC D11 VC D12 VF D11 Operating Manual...

Страница 169: ...iple Wavelength Detectors VC D11 VC D12 VF D11 Operating Manual Page 169 9 Specifications This chapter provides the physical and performance specifications including information about the materials us...

Страница 170: ...erium lamp Wavelength validation Internal validation with holmium oxide glass filter Signal channels VC detectors 8 signal channels VF detector 10 signal channels Data collection rate VC detectors Adj...

Страница 171: ...UV Lamp turned on Temperature conditions See below this table USB 1 USB port USB 2 0 B type connector 1 USB hub with 3 ports USB 2 0 A type connectors System Interlink 2 System Interlink ports RJ45 8...

Страница 172: ...he ambient temperature to 1 C hour All performance tests should be done with a completely warmed up optical unit used lamps turned on for more than two hours and new unused lamps turned on for more th...

Страница 173: ...Standard flow cell SST 10 mm 13 L 12 MPa 80 C Stainless steel fused silica PTFE PEEK titanium No Semi analytical flow cell SST 7 mm 5 L 12 MPa 80 C Stainless steel fused silica PTFE PEEK titanium No...

Страница 174: ...mperature 5 C 35 C Ambient storage temperature 20 C 45 C Ambient operating humidity 20 80 relative humidity non condensing Ambient storage humidity Maximum 60 relative humidity non condensing Operatin...

Страница 175: ...VF D11 Operating Manual Page 175 10 Accessories Consumables and Replacement Parts This chapter describes the standard accessories that are shipped with the device and the accessories that are availab...

Страница 176: ...rated only with the replacement parts and additional components options and peripherals specifically authorized and qualified by Thermo Fisher Scientific Accessories consumables and replacement parts...

Страница 177: ...formation in this manual See the content list included in the kit for the most recent information about the kit content at the time when the device is shipped Item Quantity in shipment Viper capillary...

Страница 178: ...ow cell SST 7 mm 6083 0530 Standard biocompatible flow cell PEEK 10 mm 6083 0540 Semi micro biocompatible flow cell MP35N 7 mm 6083 0550 Diagnostic cell 6083 0570 Manual inject and flush kit for flow...

Страница 179: ...or the following flow cells Standard Semi analytical Semi micro Semi micro biocompatible 6083 7950 6083 2300 Viper capillary I D x length 0 175 x 300 mm PEEK For the standard biocompatible flow cells...

Страница 180: ...5 m 6720 8910 USB cable type A to type B high speed USB 2 0 Cable length 1 m 6035 9035 USB cable type A to type B high speed USB 2 0 Cable length 5 m 6911 0002 Power cords Description Part No Power co...

Страница 181: ...11 Appendix Diode Array Multiple Wavelength Detectors VC D11 VC D12 VF D11 Operating Manual Page 181 11 Appendix This chapter provides additional information about compliance and UV cutoff wavelengths...

Страница 182: ...se of certain hazardous substances in electrical and electronic equipment The CE mark on the device indicates that the product is compliant with the directive China RoHS regulations Measures for Admin...

Страница 183: ...a Class A digital device pursuant to part 15 of the U S FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercia...

Страница 184: ...VF D11 Operating Manual 11 1 5 Manual Release History Revision Covering 2 0 VC D11 A 01 VC D12 A 01 VF D11 A 01 1 0 VF D11 A 01 The instructions were prepared in English original instructions Other l...

Страница 185: ...hey may also contain salts such as sodium hydroxide NaOH Most solvents have a UV cutoff wavelength within the spectral range of the device For optimum measurement results perform quantitative measurem...

Страница 186: ...11 Appendix Page 186 Diode Array Multiple Wavelength Detectors VC D11 VC D12 VF D11 Operating Manual...

Страница 187: ...oncentration 28 Chromeleon 41 audit trail 158 device setup 80 module setup 80 parameters 95 Predictive Performance 121 Smart Shutdown 108 Smart Standby 108 Smart Startup 89 cleaning 119 condensation 5...

Страница 188: ...apillaries and tubing 69 safety guidelines 50 site requirements 53 system 51 intended use 20 interior view 36 Interlink 57 K keypad 84 UV 85 VIS 85 L lamp 124 UV 95 VIS 95 lamp replacement 124 deuteri...

Страница 189: ...eference bandwidth 97 100 106 reference wavelength 97 100 105 regulatory compliance 30 remove slide in module 152 reordering 175 replacement parts 175 179 response time 97 100 102 optimization 102 res...

Страница 190: ...84 86 158 Status LED 84 86 158 Status LED 84 86 158 system arrangement 56 system equilibration 89 System Interlink 57 T transport 148 troubleshooting 157 general information 158 messages 160 tubing 72...

Страница 191: ...Index Diode Array Multiple Wavelength Detectors VC D11 VC D12 VF D11 Operating Manual Page 191...

Страница 192: ...Thermo Fisher Scientific Inc 168 Third Avenue Waltham Massachusetts 02451 USA www thermofisher com...

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