ThermoFisher Scientific QuantStudio 1, Инструкция по установке, эксплуатации и обслуживанию

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Perform a custom dye calibration
1.
Load the plate into the instrument.
2.
In the instrument home screen, touch Settings
4
Maintenance and
Service
4
Calibrations
4
Custom
4
Custom Dye .
3.
Touch the custom dye to calibrate.
4.
Review the custom dye information, ( optional ) make changes, then touch Update .
5.
Enter the calibration temperature.
6.
( Optional ) Touch Reagents , then enter reagent information.
7.
Touch Start .
8.
When the run is complete and the screen displays Calibration Complete , touch View
Results
4
Details .
9.
Review the plot. Passing calibration results show uniform signals with peaks that are aligned with
the dye wavelength.
Peak filter
Filter wavelength
[1]
Excitation Emission
x1-m1 470 ± 15 nm 520 ± 15 nm
x2-m2 520 ± 10 nm 558 ± 12 nm
x4-m4 580 ± 10 nm 623 ± 14 nm
x5-m5 640 ± 10 nm 682 ± 14 nm
[1]
The central wavelengths are the optimized wavelengths.
Figure 2 Example dye calibration plot
Note:
The peaks for your dye may align with a different filter set.
Chapter 5
Calibrate and verify instrument performance
Calibrate custom dyes
5
QuantStudio ™ 1 Plus Real-Time PCR System Installation, Use, and Maintenance Guide
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