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iSort
™
Automated Cell Sorter User Guide
17
Operating environment and site requirements
•
The iSort
™
Automated Cell Sorter has a footprint (W × D × H) of
approximately 67 cm × 41 cm × 49 cm (26.5 in × 16 in × 19 in) with the fluidics
compartment door and the top cover closed.
With the top cover open, the height of the instrument is 74 cm (29 in).
With the fluidics compartment door open, the entire system requires a total
bench width of ~135 cm (53.2 in).
•
The setup for the iSort
™
Automated Cell Sorter is shown below (top down
view). The gray rectangles in the diagram represent the four handholds in the
base that are used when lifting the instrument out of the box.
Touch-screen monitor
Sorting compartment
Fluidics compartment
IMPORTANT!
Do
not
lift the iSort
™
Automated Cell Sorter from the fluidics
compartment. Lifting from the fluidics compartment can damage the instrument.
Lift the instrument using the designated handholds.
•
The bench or table top must be able to accommodate 43 kg (95 lbs), the weight
of the instrument plus the weight of the full 2-liter fluid bottles (sheath fluid
and waste).
•
The iSort
™
Automated Cell Sorter should be placed on a level surface away
from vibrations from other pieces of equipment. Tabletop centrifuges, vortex
mixers, and other laboratory equipment can vibrate the instrument during a
run and impact instrument performance.
•
The area should be free of excessive dust or moisture. Position the instrument
so that the power button and main power supply can be reached easily.
•
Allow at least 6.5 cm (2.5 in) free space at the back of the instrument to allow
for proper ventilation and prevent overheating of electronic components.
•
Ambient temperature range: 18°–25°C (64°–77°F)
•
Storage temperature range: 5°–35°C (41°–95°F)
•
Relative humidity range: 20–70%
•
Electrical input: 100–240 VAC, 4.0 A
•
Frequency: 50–60 Hz
IMPORTANT!
Cell sorting creates aerosols. Exposure to and inhalation of aerosols
can potentially be hazardous depending on the cell types you are using. Consult
your institution’s safety officer for guidelines related to your individual samples.
The iSort
™
Automated Cell Sorter may need to be placed in a biosafety cabinet,
depending on your institutions’ safety requirements. The use of a filtration system
(such as the Porta PlumeSafe
™
604 system from Buffalo Filter; page 8) may also be
required to meet BSL-2 safety requirements of your institution.
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