INSTRUCTIONS
Pierce Biotechnology
PO Box 117
(815) 968-0747
thermofisher.com
3747 N. Meridian Road
Rockford, lL 61105 USA
(815) 968-7316 fax
Number
Description
89877
Zeba Spin Desalting Columns, Micro (75µL),
25 columns, for 2-12µL samples
89878
Zeba Spin Desalting Columns, Micro (75µL),
50 columns, for 2-12µL samples
89882
Zeba Spin Desalting Columns, 0.5mL,
25 columns, for 30-130µL samples
89883
Zeba Spin Desalting Columns, 0.5mL,
50 columns, for 30-130µL samples
89889
Zeba Spin Desalting Columns, 2mL,
5 columns, for 200-700µL samples
89890
Zeba Spin Desalting Columns, 2mL,
25 columns, for 200-700µL samples
89891
Zeba Spin Desalting Columns, 5mL,
5 columns, for 500-2000µL samples
89892
Zeba Spin Desalting Columns, 5mL,
25 columns, for 500-2000µL samples
89893
Zeba Spin Desalting Columns, 10mL,
5 columns, for 700-4000µL samples
89894
Zeba Spin Desalting Columns, 10mL,
25 columns, for 700-4000µL samples
89807
Zeba Spin Desalting Plates, 96-well,
2 plates
89808
Zeba Spin Desalting Plates, 96-well,
4 plates
Note:
These products are recommended for processing compounds > 7000Da. The resin slurry is
supplied in 0.05% sodium azide.
Storage:
Upon receipt store at 4
°
C. Product shipped at ambient temperature.
Introduction
The Thermo Scientific™ Zeba™ Spin Desalting Columns contain a high-performance resin that offers exceptional desalting
or buffer-exchange for protein samples. Samples volumes between 2µL-4mL and
containing as low as 20µg of protein/mL
can be processed with unsurpassed protein recovery and
≥
95% retention of salts and other small molecules (< 1000Da).
These columns require no chromatography system. The spin-column method eliminates waiting for samples to emerge by
gravity flow and the subsequent monitoring of fractions for protein recovery.
Important Product Information
•
The Zeba Spin Desalting Columns and Plates contain a size-exclusion chromatographic resin to separate proteins from
small molecules. As with all size exclusion-based separation, the amount of small molecule removed and protein
recovered are affected by the nature of the molecules and volume of sample. The sample volumes recommended provide
exceptional removal of a variety of small molecules (typically > 95% for molecules < 1000Da); however, proteins and
small molecules often behave differently than predicted because of a variety of factors such as hydrophobicity,
secondary structure and interactions. Therefore, some optimization of sample volume might be required to achieve
optimal performance for each specific sample. In general, reducing the sample volume added to the column increases
small molecule removal, and increasing sample volume maximizes protein recovery.
•
Also available are Zeba Spin Desalting Columns and Plates with a 40K MWCO, which enable removal of salts and other
small molecules < 2000Da and recovery of proteins and other macromolecules > 40,000Da.
Pub. No. MAN0015751
Pub. Part No. 2162579.1
Rev A.0
Zeba Spin Desalting Columns and Plates,
7K MWCO
