Thermo Scientific VF-F20-A, Руководство по эксплуатации

Страница: 139 / 240

6
•
Operation
Integral Fraction Collector FT (VF-F20-A) Operating Manual Page 139
•
Make sure that the temperature of the column does not exceed
40 °C.
•
When resuming operation, let the flow equilibrate and verify that
the operating parameters for the other system modules are set as
required before proceeding.
6.10.2 Long-Term Shutdown
Shutting Down the device
TIP Shutting down the device affects the operation of the system. When
shutting down the device, also observe the shutting down instructions
for the other Vanquish system modules and take appropriate action
(refer to the
Operating Manuals for the modules).
To interrupt operation for a longer period, follow the instructions below.
1. Check that the fraction collection valve is set to Collect position.
2. Flush the system with an appropriate, pure solvent (minimum HPLC-
grade).
Observe the following:
Situation after Shutdown If no additive is used If an addititive is used
Device remains in the
laboratory after shutdown Flush the system, for
example with methanol.
100% acetonitrile should
not be used. Flush the system with
several volumes (for
example, 1.0 mL/min for 10
minutes with the standard
system) of methanol and
water (50:50) to prevent
salt buildup in the fluidics. If
the solvents in the device
are not miscible with water,
use an appropriate
intermediate solvent.