Thermo Scientific TSQ 8000, Руководство пользователя

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5
Optimizing Your Method
Optimizing an SRM Method
92 TSQ 8000 Evo User Guide Thermo Scientific
Use the highest ion source temperature setting that allows adequate response for targeted
analytes in order to improve response for high-boiling compounds and prevent column bleed
and matrix compounds from dirtying the ion source.
Optimizing an SRM Method
The following actions might improve your results when using an SRM method:
• Use timed SRM to optimize segments automatically and possibly improve dwell time and
number of points across the peak.
• Reduce the Retention Time window if possible. If your sample's retention time is well
known the scan window can be reduced. If this is in a high-density compound area it can
dramatically improve the ion signal and number of points across the peak.
• Use AutoSRM to optimize collision energy and retention times. This can reduce errors in
collision energy and improve confidence in retention times to allow shorter windows.
• Choose the optimum fragment ion for study if allowed by your method. Some fragments
are higher in intensity and have less interference associated with their transition.
Optimizing an NCI Tune
Use the following workflow to adjust the low mass when you create an NCI tune.
1. Click Manual Tune button on the TSQ Series Dashboard.
Figure 89. Finding Manual Tune on the TSQ Series Dashboard
Note The transfer line temperature and ion source temperature should be similar to avoid
contaminating the ion source.