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8

 • 

Troubleshooting

Page 138

Variable Wavelength Detectors (VC-D40, VF-D40) 

Operating Manual

8.1

General Information about Troubleshooting

The following features help you to identify and eliminate the source for
problems that may arise during operation of the device.

TIP

 For information about operating issues that might occur during the

operation of a Vanquish system, refer to the 

Vanquish System Operating

Manual

.

If you are unable to resolve a problem following the instructions given
here or if you experience problems that are not covered in this section,
contact Thermo Fisher Scientific Technical Support for assistance. See
the contact information at the beginning of this manual.

To facilitate device identification, have the serial number and technical
name available when communicating with Thermo Fisher Scientific.

Status Indicators

The status indicator LED bar on the front side of the device and the

STATUS

 LED on the keypad inside provide quick visual feedback on the

operational status of the device. If the device firmware detects a
problem, the status indicators are red. The problem is reported to the
Chromeleon software and a message appears in the Audit Trail.

Alarms

Leaks are a potential safety issue. Therefore, if a leak sensor detects
leakage, beeping starts to alert you in addition to the message in the
Chromeleon Audit Trail and the status indicators changing to red. Follow
the instructions in this manual to find and eliminate the source for the
leakage.

Chromeleon Audit Trail Messages

If the device firmware detects a problem, the problem is reported to the
Chromeleon software.

The Chromeleon software logs information about all events related to
instrument operation for the current day in an Audit Trail. The Audit
Trail is named with the current date, using the format yyyymmdd. For
example, the Audit Trail for May 15, 2019, is named 20190515.

The Instrument Audit Trails can be found on the ePanel Set (Audit
ePanel). In addition, Audit Trails for each instrument are available in the
Console Data view, in the folder of the Instrument.

Содержание Thermo Scientific Vanquish VC-D40

Страница 1: ...thermoscientific Vanquish Variable Wavelength Detectors VC D40 VF D40 Operating Manual 4820 7701 EN Revision 2 0 December 2019 ...

Страница 2: ...nual is provided as is The contents of this manual are subject to being changed without notice in future revisions Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free Thermo Fisher Scientific Inc assumes no responsibility and will not be liable for any errors omissions damage or loss that might result from any use of this document even if the...

Страница 3: ...tific sales organization For contact information go to Contact Us on http www thermofisher com Technical Assistance For technical support for HPLC products contact your local Thermo Fisher Scientific support organization For contact information go to Contact Us on http www thermofisher com Contacting Us Variable Wavelength Detectors VC D40 VF D40 Operating Manual Page 3 ...

Страница 4: ...Contacting Us Page 4 Variable Wavelength Detectors VC D40 VF D40 Operating Manual ...

Страница 5: ... Safety Symbols on the Device 19 2 1 4 Rating Plate 19 2 2 Intended Use 20 2 3 Safety Precautions 21 2 3 1 General Safety Information 21 2 3 2 Qualification of the Personnel 21 2 3 3 Personal Protective Equipment 22 2 3 4 Electrical Safety Precautions 23 2 3 5 General Residual Hazards 23 2 3 6 In Case of Emergency 26 2 4 Solvent and Additive Information 27 2 4 1 General Compatibility 27 2 4 2 Allo...

Страница 6: ...rd 55 5 6 Installing a VIS Lamp Tungsten Lamp Optional 56 5 7 Installing a Flow Cell 59 5 7 1 Removing the Diagnostic Cell 59 5 7 2 Installing a Flow Cell without Connection Unit 60 5 7 3 Installing a Flow Cell with Connection Unit 60 5 8 Setting Up the Flow Connections 62 5 8 1 General Information and Guidelines 62 5 8 2 Guiding Capillaries and Tubing Through the System 63 5 8 3 Installing the Pa...

Страница 7: ...2 6 8 5 Response Time and Peak Width 92 6 8 6 Baseline Behavior 93 6 9 Shutting Down the Device 94 6 9 1 Short Term Shutdown Interruption of Operation 94 6 9 2 Long Term Shutdown 95 7 Maintenance and Service 99 7 1 Introduction to Maintenance and Service 100 7 2 Safety Guidelines for Maintenance and Service 101 7 3 General Rules for Maintenance and Service 103 7 4 Routine and Preventive Maintenanc...

Страница 8: ... Slide In Module 131 7 13 2 Returning the Slide In Module 132 7 13 3 Installing the Slide In Module 133 7 13 4 Setting Up the Slide In Module 135 8 Troubleshooting 137 8 1 General Information about Troubleshooting 138 8 2 Messages 140 8 3 Checking the Flow Cell 145 8 4 Resolving Liquid Leaks 146 9 Specifications 149 9 1 Performance Specifications 150 9 1 1 Detector 150 9 1 2 Flow Cells 152 9 2 Phy...

Страница 9: ...Wavelength Detectors VC D40 VF D40 Operating Manual Page 9 11 1 3 FCC Compliance 163 11 1 4 NIST Compliance 163 11 1 5 Manual Release History 163 11 2 UV Cutoff Wavelengths of Solvents 165 11 3 Digital I O 166 Index 169 ...

Страница 10: ...Contents Page 10 Variable Wavelength Detectors VC D40 VF D40 Operating Manual ...

Страница 11: ... Detectors VC D40 VF D40 Operating Manual Page 11 1 Using this Manual This chapter provides information about this manual the conventions used throughout the manual and the reference documentation that is available in addition to this manual ...

Страница 12: ...an prevent personal injury damage to the device or loss of data when followed properly Note the following The device configuration may vary therefore not all descriptions necessarily apply to your particular device If some detail applies to only one model or variant the model or variant is identified by name Illustrations in this manual are provided for basic understanding They can vary from the a...

Страница 13: ...icular section or procedure appear in the section or procedure to which they apply They appear different from the main flow of text Safety messages are often preceded by an alert symbol and or alert word The alert word appears in uppercase letters and in bold type Make sure that you understand and follow all safety messages presented in this manual 1 2 2 Special Notices and Informational Notes Spe...

Страница 14: ...rams Thermo Chromeleon 7 Services Manager Start Instrument Controller References and Messages References to additional documentation appear italicized Messages that appear on the screen are identified by quotation marks Viewpoint If not otherwise stated the expressions left and right in this manual always refer to the viewpoint of a person that is facing the device from the front Particularly Impo...

Страница 15: ...or Adobe Acrobat is required Software Documentation Additional software documentation includes the following Chromeleon Help and documents The Chromeleon Help provides extensive information and comprehensive reference material for all aspects of the software In addition the following documentation is available availability depends on the software version Installation Guide For basic information ab...

Страница 16: ...F D40 Operating Manual TIP The Chromeleon Help and documents are included in the software shipment Third Party Documentation Refer also to the user documentation provided by the manufacturers of third party components and materials for example Safety Data Sheets SDSs ...

Страница 17: ...2 Safety Variable Wavelength Detectors VC D40 VF D40 Operating Manual Page 17 2 Safety This chapter provides general and specific safety information and informs about the intended use of the device ...

Страница 18: ... CAUTION Indicates a hazardous situation that if not avoided could result in minor or moderate injury WARNING Indicates a hazardous situation that if not avoided could result in serious injury 2 1 2 Observing this Manual Observe the following Before installing or operating the device read this manual carefully to be familiar with the device and this manual The manual contains important information...

Страница 19: ...ion Do not touch these surfaces while they are heated up Indicates that the UV radiation produced by the deuterium lamp in the device may be harmful to eyes and skin Do not look directly into the light produced by the deuterium lamp Never operate the lamp outside the device 2 1 4 Rating Plate The rating plate is present on the device near the electrical connections The rating plate indicates the s...

Страница 20: ...sh system are intended to be used as General Laboratory Equipment GLE They are not intended for use in diagnostic procedures Laboratory Practice Thermo Fisher Scientific recommends that the laboratory in which the Vanquish system is used follow best practices for LC analyses This includes among others Using appropriate standards Regularly running calibration Establishing shelf life limits and foll...

Страница 21: ...for the device by Thermo Fisher Scientific Perform only the procedures that are described in this operating manual and in supporting documents for the device Follow all instructions step by step and use the tools recommended for the procedure Open the enclosure of the device and other components only if specifically instructed to do so in this manual Thermo Fisher Scientific cannot be held liable ...

Страница 22: ...nted by the device and the substances they are using All users should observe the related Safety Data Sheets SDSs 2 3 3 Personal Protective Equipment Wear personal protective equipment and follow good laboratory practice to protect you from hazardous substances The appropriate equipment depends on the hazard For advice on the hazards and the equipment required for the substances you are using refe...

Страница 23: ...or grounding connections If you suspect any kind of electrical damage disconnect the power cord and contact Thermo Fisher Scientific Technical Support for assistance Do not open the housing or remove protective panels unless specifically instructed to do so in this manual Do not place liquid reservoirs directly upon the device Liquid might leak into the device and get into contact with electronic ...

Страница 24: ...rmful substances Make sure that the installation site is well ventilated Dispose of hazardous waste in an environmentally safe manner that is consistent with local regulations Follow a regulated approved waste disposal program WARNING Biohazard Biohazardous material for example microorganisms cell cultures tissues body fluids and other biological agents can transmit infectious diseases To avoid in...

Страница 25: ...rofuran THF can cause PEEK to swell Concentrated acids such as sulfuric acid and nitric acid or a mixture of hexane ethyl acetate and methanol can attack PEEK Swelling or attack is not a problem with brief flushing procedures For more information refer to the technical literature on the chemical resistance of PEEK CAUTION Allergic Reaction Some capillaries in the Vanquish system are made of MP35N ...

Страница 26: ...2 Safety Page 26 Variable Wavelength Detectors VC D40 VF D40 Operating Manual 2 3 6 In Case of Emergency WARNING Safety Hazard In case of emergency disconnect the device from the power line ...

Страница 27: ... only solvents and additives that are compatible with all parts in the flow path TIP In a Vanquish Core system normal phase NP compatible solvents and additives may be used if the VC pumps and the VC autosamplers are modified with the components from the Normal Phase NP kit Refer to the Operating Manuals for the pumps and autosamplers NOTICE If the system flow path includes a Vanquish charged aero...

Страница 28: ...ing the system disconnect the detector from the system Mobile phases containing ammonium hydroxide In rare cases a shortened lifetime of reversed phase UHMW PE piston seals has been observed with high pH ammonium hydroxide containing mobile phases and prolonged exposure Vanquish Horizon Vanquish Flex 2 12 2 4 3 Allowed Concentrations Allowed concentrations standard system configuration System Stan...

Страница 29: ...her modules in the Vanquish system refer to the Specifications chapter in the Operating Manual for the modules Follow any specific recommendations presented in other sections of this manual Refer also to the operating manuals for all modules in the Vanquish system They may provide additional guidelines and information Observe the general guidelines and recommendations on the use of solvents and ad...

Страница 30: ...nges that you make to the device may void compliance with one or more of these EMC and safety standards Changes to the device include replacing a part or adding components options or peripherals not specifically authorized and qualified for the product by Thermo Fisher Scientific To ensure continued compliance with EMC and safety standards replacement parts and additional components options and pe...

Страница 31: ...3 Device Overview Variable Wavelength Detectors VC D40 VF D40 Operating Manual Page 31 3 Device Overview This chapter introduces you to the device and the main components ...

Страница 32: ...at up to two VC D40 or four VF D40 different wavelengths Data collection rates of up to 125 Hz VC D40 and up to 250 Hz VF D40 under Chromeleon 7 2 software or later in single channel mode Spectra measurements Two optical filters for eliminating higher order light To ensure optimal measurement results over the entire wavelength range the optical filters automatically move into the light path also i...

Страница 33: ...mirror no 7 and on to the grating no 8 where the light beam is diffracted The angular position of the grating determines the selected wavelength that is focused by the mirror no 9 and directed to the beamsplitter no 10 and flow cell no 13 The beamsplitter no 10 directs part of the light beam to the reference diode no 11 If the flow cell is removed a safety shutter no 12 blocks the light beam to pr...

Страница 34: ...ure 1 Operating principle of the optics No Description 1 VIS lamp tungsten lamp 2 Mirror VIS 3 UV lamp deuterium lamp 4 Mirror combined light 5 Shutter 6 Entrance slit 7 Mirror 8 Grating 9 Mirror 10 Beamsplitter 11 Reference photodiode 12 Safety shutter 13 Flow cell 14 Measurement photodiode ...

Страница 35: ...ith status indicators 3 Screw holes for connection unit left side installation 4 Flow cell with connection unit 5 Connection unit 6 Leak tray 7 Type label indicating the module name serial number part number and revision number if any 8 Drain port 9 Leak sensor 10 Partition panel The recesses in the partition panel are used to route capillaries with the help of special plugs see Installing the Par...

Страница 36: ...low cell labels are present on the flow cell which contain information such as flow cell type part number and serial number Flow Cell Identification Chip An identification ID chip on the flow cell stores information including the flow cell type and the serial number of the flow cell The ID chip also stores data during operation such as the exposure time to the light When the flow cell is installed...

Страница 37: ...r is shipped with a diagnostic cell Replace the diagnostic cell with a flow cell that is appropriate for your application Depending on the application needs different types of flow cells are available for the detector For flow cell ordering information see Optional Accessories page 158 For the flow cell specifications including for example materials in the flow path and pressure limit see Flow Cel...

Страница 38: ...ed if at least one of your detection wavelengths is within the range indicated in the left column At least one detection wavelength Recommended lamp s 345 nm UV Between 346 and 670 nm UV and VIS turn on both lamps 670 nm VIS Identification Chip Each lamp is equipped with an identification ID chip The ID chip stores information about the lamp including the number of lamp ignitions and the operating...

Страница 39: ...iquid leaks from the flow connections The liquid is collected in the leak tray and guided to the drain port From the drain port the liquid is discharged to waste through the drain system of the Vanquish system When the leak sensor detects leakage the status indicators change to red and beeping starts to alert you Follow the instructions in this manual to find and eliminate the source for the leaka...

Страница 40: ... automated sample analysis with the Chromeleon software refer to the Vanquish System Operating Manual Details on control and operation of the device are available in the Chromeleon Help TIP The device can be operated also with other data systems such as Thermo ScientificTM XcaliburTM In this case installation of additional software is required in addition to the data system software For details co...

Страница 41: ...4 Unpacking Variable Wavelength Detectors VC D40 VF D40 Operating Manual Page 41 4 Unpacking This chapter provides information for unpacking the device and informs you about the scope of delivery ...

Страница 42: ...ulky for one person alone to handle safely To avoid personal injury or damage to the device observe the following guidelines Physical handling of the device including lifting or moving requires a team effort of two persons A team effort is in particular required when lifting the device into the system stack or when removing it Use the carrying handles that were shipped with the device to move or t...

Страница 43: ...d place it in the system stack see System Arrangement page 52 7 On each carrying handle loosen the attachment screw until the carrying handle is moveable in the rail Do not remove the screws from the carrying handles completely 8 Slide off the carrying handles from the rails towards the rear of the device Figure 5 Sliding off the carrying handle from the left rail TIP Keep the shipping container t...

Страница 44: ... D40 Operating Manual 4 2 Scope of Delivery The following items are included in the delivery Detector Ship Kit Operating manual Power cord For information on contents of the ship kit or reordering parts see Accessories Consumables and Replacement Parts page 155 ...

Страница 45: ...ors VC D40 VF D40 Operating Manual Page 45 5 Installation This chapter specifies the requirements for the installation site and describes how to set up install and configure the device in the Vanquish system and in the chromatography software ...

Страница 46: ...eam effort is in particular required when lifting the device into the system stack or when removing it Use the carrying handles that were shipped with the device to move or transport the device Never move or lift the device by the front doors This will damage the doors or the device CAUTION Electric Shock or Damage to the Device After the power to the device is turned off the device is still energ...

Страница 47: ...on page 46 and Site Requirements page 49 2 Set up the device hardware See Setting Up the Hardware page 52 3 Set up the flow connections See Setting Up the Flow Connections page 62 4 Turn on the device See Turning On the Device page 73 TIP Before turning on the power to a Vanquish system module for the first time verify that the chromatography software is installed on the data system computer When ...

Страница 48: ...ructions provide information about the parameters to be adapted and the required settings 7 Recommended Perform Operational Qualification The qualification kit includes all materials required for the qualification and detailed instructions Moving the Device after Installation If you have to move the device after it has been set up and installed in the Vanquish system prepare the device for transpo...

Страница 49: ...g Manuals for the other modules in the Vanquish system For general residual hazards see General Residual Hazards page 23 5 3 1 Power Considerations The power supply of the device has wide ranging capability accepting any line voltage in the range specified for the device CAUTION Electric Shock or Damage to the Device Connecting the device to a line voltage higher or lower than specified could resu...

Страница 50: ...ce from the power line WARNING Electric Shock or Damage to a Product Misuse of the power cords could cause personal injury or damage the instrument Use the power cords provided by Thermo Fisher Scientific only for the purpose for which they are intended Do not use them for any other purpose for example for connecting other instruments 5 3 3 Condensation NOTICE Condensation in the device can damage...

Страница 51: ...51 5 4 Accessing the Interior Components To access the interior components in the device open the front doors To allow easy access from the front the user accessible components and flow connections in the device are located directly behind the doors Figure 6 Opening the front doors ...

Страница 52: ...ation about the device connectors and cables 5 5 1 System Arrangement The device is part of the Vanquish system The system modules are typically arranged in a system stack with the arrangement depending on the system configuration Figure 7 Vanquish system standard configuration example No Description 1 Solvent Rack 2 Detector 3 Autosampler 4 Pump 5 System Base 6 Column Compartment ...

Страница 53: ... Connecting the Device Device Connectors The following connectors are provided on the device Figure 8 Electrical connectors on the right side of the detector No Description 1 Rating plate indicating the serial number part number module name revision number if any line and fuse rating and the manufacturer s address 2 Main power switch on off control 3 Fuse holder 4 Power inlet connector ...

Страница 54: ...SB Universal Serial Bus port B type connector Allows connection to other modules in the Vanquish system or the computer on which the data management system is installed such as the Chromeleon software TIP Thermo Fisher Scientific recommends using the USB ports only as described above If the USB ports are used for any other purpose Thermo Fisher Scientific cannot ensure proper functionality Follow ...

Страница 55: ...ine be sure that no condensation is present in the devices If you suspect that condensation is present allow the device to warm up to room temperature slowly Wait until the condensation is completely gone before proceeding 1 Verify that the power switch on the device is set to OFF 2 Connect the power cord to the power inlet connector on the device 3 Connect the free end of the power cord to an app...

Страница 56: ...nal VIS lamp Parts required VIS lamp Preparations 1 Turn off the device Follow these steps Figure 9 VIS lamp No Description 1 VIS lamp locating pin 2 VIS lamp 3 VIS lamp attachment screw 4 VIS lamp connector 1 Open the doors 2 Turn the two screws on the lamp house cover counterclockwise until the lamp house cover is loose Do not remove the screws completely from the lamp house cover ...

Страница 57: ...n the two screws to attach the lamp to the lamp house 7 Connect the lamp connector 8 Mind the routing of the lamp cables Position the lamp cables as indicated in Figure 9 VIS lamp page 56 to prevent the cable from being pinched under the lamp house cover 9 Install the lamp house cover and fasten the screws on the lamp house cover 10 Turn on the device with its main power switch 11 If you install a...

Страница 58: ... noise and strong baseline fluctuations may occur Before beginning an analysis or performing a wavelength validation allow the new lamp to run until the noise is reduced and the baseline is stable which typically takes 24 hours 13 Perform a wavelength validation see Performing a Wavelength Validation and Calibration page 119 ...

Страница 59: ...tector Hold flow cells by the flow cell handle To avoid damage and or contamination of the optical block of the flow cell do not touch the optical block On the rear side of the flow cell the contact pads for the identification chip are located Never touch the contact pads Avoid damage to the electronics of the ID chip NOTICE Flow Cell Opening The optical ports and the contact pad for the identific...

Страница 60: ...two flow cell screws hand tight 3 Set up the flow connections to the flow cell see Setting Up the Flow Connections page 62 4 Perform wavelength validation see Performing a Wavelength Validation and Calibration page 119 TIP If the wavelength validation fails perform a calibration of wavelengths 5 7 3 Installing a Flow Cell with Connection Unit Parts required Flow cell as required by the application...

Страница 61: ... unit screw knurl 3 Tighten the connection unit screw hand tight 4 Push the flow cell completely into the flow cell opening 5 Tighten the two flow cell screws hand tight 6 Set up the flow connections on the flow cell see Setting Up the Flow Connections page 62 7 Perform wavelength validation see Performing a Wavelength Validation and Calibration page 119 TIP If the wavelength validation fails perf...

Страница 62: ...ry statements presented in Safety Precautions page 21 NOTICE Particulate matter from other system modules and components can deposit in the flow cell and clog it Before you connect the flow cell to the flow path make sure that you thoroughly flush the modules in the system flow path upstream of the device to waste When you install devices or components to the system always flush them to waste befo...

Страница 63: ... collectors downstream of the flow cell operating those under flow may result in pressure spikes that can destroy the flow cell Ensure that pressure spikes do not exceed the flow cell pressure specification Follow these steps To set up the flow connections and complete the installation of the device follow these steps 1 Set up the flow connections to the flow cell see Flow Connections to the Flow ...

Страница 64: ...ng up to three solvent lines 2 Solvent tubing up to three solvent lines 3 Wash liquid tubing seal wash autosampler needle wash 4 Detector waste line Tubing brackets are available for holding the tubing in place Slip the bracket side onto the drain pipe Figure 13 Tubing bracket left tubing bracket installed right Dual System Arrangements The number of tubes may exceed the capacity that the tubing g...

Страница 65: ...o types of partition panel plugs available in the detector ship kit Figure 14 Plugs available for the partition panel No Description 1 Plug with slit for guiding capillaries with small outer diameter such as uninsulated capillaries 2 Rotating plug for guiding capillaries that do not fit in the plug with slit including insulated capillaries Installing the plug with slit 1 On the detector partition ...

Страница 66: ...lug in order to route the capillary through the plug turn the rotating plug toward the front 3 To secure the capillary turn the rotating plug toward the partition panel to close the opening of the rotating plug Figure 17 Using the rotating plug 5 8 4 Connecting Fittings Capillaries and Tubing The inlet and outlet ports of the flow cells support the following capillary fittings Viper nanoViper Stan...

Страница 67: ...o invalid test results Do not install capillaries or tubes that are stressed nicked kinked or otherwise damaged Install capillaries and fittings only at the positions for which they are intended 5 8 4 2 Connecting Viper Capillaries This section describes how to connect Viper capillaries All Viper flow connections in the Vanquish system are designed to be finger tight To connect Viper capillaries w...

Страница 68: ...is slot and attach them again later 3 Check whether the connection leaks If leakage exists follow the steps further down Resolving Leakage of Viper Fittings with Knurls 1 Tighten the connection a little more 2 If leakage continues remove the capillary 3 Clean the capillary ends carefully by using a lint free tissue wetted with isopropanol 4 Reinstall the capillary 5 If the connection continues to ...

Страница 69: ...see Replacing the Doors page 126 1 Flush the system modules and capillaries upstream of the device to waste before you connect the flow cell to the system flow path Refer to the Vanquish System Operating Manual 2 On the flow cell remove the plugs from the flow cell inlet and outlet TIP Store the plugs of the flow cell for example in the flow cell packaging to have them easily available when storin...

Страница 70: ...on between the column compartment and the flow cell as short as possible to minimize peak dispersion i e peak broadening effects due to extra dispersion volume 2 If the column compartment is located to the right of the detector Route the capillary through the bottom recess in the partition panel Make sure that you use the suitable partition panel plug for the capillary TIP For capillaries with sma...

Страница 71: ...el 3 Route the waste line through the tubing guides of the system modules below the detector to the Vanquish system base 3 4 On the system base route the detector waste line through the dedicated detector waste outlet to waste and connect the detector waste line to the waste container as described in the Vanquish System Operating Manual TIP The waste line should go straight to the system base and ...

Страница 72: ...of the waste line is present at any point in the flow path 5 8 6 Guiding Liquid Leaks to Waste Leaking liquids of the device are collected in the leak tray where they flow off through the chase on the right side of the leak tray to the drain system For information about how the liquid is discharged to waste through the Vanquish drain system refer to the Vanquish System Operating Manual ...

Страница 73: ...tem can detect the device To turn on the power to the device follow these steps 1 Check that the power button on the front left of the Vanquish system base system power button is pressed in If the power button stands out press the power button to turn on the power on the system base 2 Turn on the device with its main power switch Turn the device off with the main power switch when instructed to do...

Страница 74: ...hat the chromatography software is already installed on the data system computer and a valid license is available For more information about setting up the Vanquish system in the software refer to the Vanquish System Operating Manual The Help for the software that you are using provides detailed information about the settings on each property page ...

Страница 75: ...peration Variable Wavelength Detectors VC D40 VF D40 Operating Manual Page 75 6 Operation This chapter describes the elements for device control provides information for routine operation and for shutdown ...

Страница 76: ...s is not the case refer to the instructions in Installation page 45 For a basic description of instrument control and automated sample analysis with the Chromeleon software refer to the Vanquish System Operating Manual Details on control and operation of the device are available in the Chromeleon Help Software descriptions in this manual refer to Chromeleon 7 Terminology may be different to that o...

Страница 77: ...ng the device pay attention to the following safety guidelines Observe all warning messages and precautionary statements presented in Safety Precautions page 21 CAUTION Hot Surfaces Surfaces inside the device may become hot during operation Touching hot parts might cause burns Allow hot surfaces to cool down before you touch them ...

Страница 78: ...nd the STATUS LED on the keypad provide a quick visual check of the operational status of the device 6 3 1 Keypad The keypad inside the device allows you to perform certain functions directly from the device When you press a button a short beep confirms that the function is performed When the device is connected in the Chromeleon software some functions may not be available from the keypad see fur...

Страница 79: ...IS The VIS button allows you to turn the VIS lamp on and off directly from the detector To turn on the VIS lamp press the VIS button The LED next to the button indicates the lamp status LED Description Off dark The VIS lamp is turned off Green flashing The VIS lamp ignites Green The VIS lamp is turned on When the device is connected in the Chromeleon software The button functionality is as follows...

Страница 80: ...t one lamp is igniting Green The device is connected and equilibrated but no data acquisition is running At least one lamp is turned on Blue running A data acquisition is running Blue An injection or sequence is running Red A problem or error has occurred For the related message check the Chromeleon Audit Trail For remedial action see Troubleshooting page 137 STATUS LED The STATUS LED on the keypa...

Страница 81: ...ollowing All modules in the Vanquish system that are connected to the system base via system interlink cables are turned on or off simultaneously when the system power button is pressed When the power is on the system power button is pressed in When the power is off the system power button stands out If the main power switch on a device is off you cannot turn on the device with the system power bu...

Страница 82: ... be filled with solvent Use solvents that are miscible with this solvent or use an appropriate intermediate solvent New flow cells are dry or filled with isopropanol when shipped To remove the isopropanol from the device components Verify that the flow cell and the column are not connected in the flow path Purge the pump and then have the pump deliver flow for a short time Verify that any air bubb...

Страница 83: ...rged aerosol detector Turning on the lamp or lamps in the UV VIS detector Monitoring the pump pressure and pressure ripple and checking that the pressure is stable and the ripple within reasonable limits for the application Monitoring the detector signal and checking whether the detector signal is stable so that the drift and signal noise are within reasonable limits for the application Performing...

Страница 84: ... in the flow cell without flow Particularly avoid leaving any aggressive solvents in the flow cell for a longer time Without flow air bubbles in the flow cell can accelerate the deposition of substances Never leave air bubbles in the flow cell If the pump flow is stopped for a longer time fill the flow cell with a high purity solvent for example isopropanol Troubleshooting flow cells If a flow cel...

Страница 85: ...mergency shut off of the lamps is triggered Allow the optics to warm up and stabilize for at least one hour until the working temperature is reached before you start analysis The optics requires some time to warm up for optimal performance before analysis TIP When the UV lamp was turned off a cooling down period of 5 minutes is required before the lamp can ignite again The detector monitors the co...

Страница 86: ...s considerably shorter than one second and should be performed when no sample components of interest are present in the flow cell While the wavelength is switched no absorption measurement takes place for a short period of time If the wavelength is switched during an analysis of a sample component the peak area changes and a correct quantification is not possible You can choose between three diffe...

Страница 87: ...e detector in the long term The multi channel operation mode is therefore only recommended for method development and peak purity analysis but not for continuous routine operation As an alternative you can switch the wavelengths as often as required in a single data channel in the Chromeleon software For further information on wavelength switching and single wavelength operation see Single Channel...

Страница 88: ... The temperature signal channel Lamphouse Temperature that is available in the Properties dialog box of the detector can be used for troubleshooting purposes The lamps of the detector are very sensitive to changes in temperature In case of baseline drift problems the lamp house temperature signal can help to find the reason for the problem Select the signal channel to record the temperature Chrome...

Страница 89: ...nd VIS lamp of the detector To turn the UV lamp on automatically after power on set AutoactivateUV_Lamp to On To turn the VIS lamp on automatically after power on set AutoactivateVisible_Lamp to On UV_VIS The UV_VIS signal channels UV_VIS_x that are available for data acquisition are listed in the dialog box for the detector in the Instrument Configuration Manager Select the required signal channe...

Страница 90: ...void exposure of the flow cell to thermal changes in the environment that may lead to a baseline drift Ensure that the operating conditions are suitable This includes Stable environmental conditions such as a stable temperature No air drafts No vibrations or mechanical shocks caused by external sources Stable backpressure and correct waste line setup For information about the UV cutoff wavelengths...

Страница 91: ...ferably select a wavelength on the absorption maximum of the sample components Avoid the wavelength range where the solvents absorb strongly for example below 220 nm for methanol and below 210 nm for acetonitrile The wavelength should always be selected above the UV cutoff of the solvent For information about the UV cutoff wavelengths of solvents see UV Cutoff Wavelengths of Solvents page 165 Wave...

Страница 92: ... and allows for a faster display of data in the Chromeleon software If any peaks of interest are less than a few seconds select a higher data collection rate 10 0 Hz for example Multiple wavelength applications In multiple wavelength applications baseline noise increases compared to single channel data acquisition This is especially the case if the data rate is set to the maximum or close to the m...

Страница 93: ...est possible combinations of data collection rate response time and peak width enable the Link data collection parameters check box in the detector settings of the Chromeleon Instrument Method Wizard or Instrument Method Editor 6 8 6 Baseline Behavior If wavelengths are switched during a single channel data acquisition the chromatogram may show baseline jumps You can define how the baseline behave...

Страница 94: ...he following Detector Type Description Charged aerosol detector Check that sufficient gas is available to continue gas flowing through the detector This is to prevent any build up of residue from solvents or analytes Gas must be flowing when pump flow is delivered to the detector UV VIS detectors The lamp s in the detector can remain turned on Variable wavelength detector and VH D10 diode array de...

Страница 95: ...estart the pump flow 5 Flush the flow cell with an appropriate solvent minimum HPLC grade Observe the following TIP With a Vanquish Core system that has been modified for using normal phase compatible solvents and additives see the information about the flushing liquid in the Considerations with Normal Phase Compatible Solvents and Additives section in Operating Manual for the pump Situation after...

Страница 96: ...n solvents containing acid can damage the flow cell Always flush the flow cell with an appropriate solvent before interrupting operation Fill the flow cell with pure isopropanol using the flushing and injection kit The flow cell should not be filled with pure water to avoid the growth of algae If you want to fill the flow cell with water you need to add 10 HPLC grade isopropanol 6 Turn off the pum...

Страница 97: ...les shall be removed from the system stack turn off all system modules with their main power switch Pressing the system power button will not be sufficient to turn off the power to the devices completely Follow the instructions in Transporting or Shipping the Device page 128 See also 2 Connecting the Inlet Capillary page 70 2 Connecting the Detector Waste Line page 71 ...

Страница 98: ...6 Operation Page 98 Variable Wavelength Detectors VC D40 VF D40 Operating Manual ...

Страница 99: ...ance and Service Variable Wavelength Detectors VC D40 VF D40 Operating Manual Page 99 7 Maintenance and Service This chapter describes the routine maintenance and the service procedures that the user may perform ...

Страница 100: ...fic for brevity referred to as Thermo Fisher Scientific service personnel The device is designed for easy maintenance and service The user serviceable parts of the device can be accessed from the front If not stated otherwise the maintenance procedures do not require that you remove the device from the system The maintenance procedures do not require that you remove the doors However it is possibl...

Страница 101: ...s that can pose health risks Solvent can spray when capillaries burst slip out of their fittings or are not properly tightened or when capillary connections are otherwise open Wear appropriate protective equipment and follow good laboratory practice Before starting maintenance or repair procedures flush out harmful substances with an appropriate solvent WARNING Tilting Liquid Reservoirs Liquids in...

Страница 102: ...e pump flow and wait until the system pressure is down to zero Unscrew the caps of the solvent reservoirs and remove the solvent lines together with the caps from the reservoirs Empty the solvent lines Refer to the Operating Manual for the pump Retighten the reservoir caps CAUTION Electric Shock or Damage to the Device After the power to the device is turned off the device is still energized as lo...

Страница 103: ...tools recommended for the procedure Before opening the flow path to replace capillaries in the system turn off the pump flow and wait until the system pressure is down to zero Dirty components can contaminate the chromatography system Contamination leads to poor performance of the modules and entire system or can even cause damage to the modules and system Therefore Always wear appropriate gloves ...

Страница 104: ...with an appropriate solvent that does not contain buffers or salts Monitor the backpressure that the flow cell is exposed to Regularly Inspect the flow connections for damage such as cracks nicks cuts or blockage Check that all warning labels are still present on the device and clearly legible If they are not contact Thermo Fisher Scientific for replacement Annually Have Thermo Fisher Scientific s...

Страница 105: ...oid open flames or exposure to excessive heat during the cleaning process Wipe the cleaned components thoroughly dry after cleaning Do not operate the device before it is completely dry NOTICE Observe the following Only use cleaning detergents that will not damage the surfaces of the system Never use sharp tools or brushes for cleaning any surfaces Do not use sprays for cleaning Prevent cleaning d...

Страница 106: ...ervice and qualification information about the device These functions are called Predictive Performance They allow you to schedule maintenance procedures based on the actual operating and usage conditions of the device On special wellness service and qualification panels you can define intervals for replacing components that are subject to wear or stress and for service procedures or qualification...

Страница 107: ... detector may result in inferior performance detector malfunction or may damage the detector Detector guaranty may be voided Use only lamps supplied or approved by Thermo Fisher Scientific for this detector 7 5 1 Replacing the UV Lamp Deuterium Lamp When The lamp is defective After a defined amount of operating hours of the lamp as a preventive measure The lamp intensity is too low for demanding a...

Страница 108: ... these steps Figure 22 UV lamp No Description 1 UV lamp connector 2 UV lamp 3 UV lamp attachment screw 4 UV lamp locating pin 1 Open the doors 2 Turn the two screws on the lamp house cover counterclockwise until the lamp house cover is loose Do not remove the screws completely from the lamp house cover ...

Страница 109: ...disconnect the connector from the lamp socket on the device 5 With your hands loosen the two screws that attach the lamp to the lamp house 6 Pull out the UV lamp 7 Inspect the new UV lamp for signs of fingerprints and dust If necessary clean the UV lamp with isopropanol and a lint free tissue before you install it 8 Align the new lamp with the locating pin When the lamp is in the correct position ...

Страница 110: ...lidation allow the new lamp to run until the noise is reduced and the baseline is stable which typically takes 24 hours 15 Perform a wavelength validation see Performing a Wavelength Validation and Calibration page 119 TIP The lamp age counter is automatically reset to the value stored on the ID chip of the lamp See also 2 Performing a Wavelength Validation and Calibration page 119 7 5 2 Replacing...

Страница 111: ...hese steps Figure 24 VIS lamp No Description 1 VIS lamp locating pin 2 VIS lamp 3 VIS lamp attachment screw 4 VIS lamp connector 1 Open the doors 2 Turn the two screws on the lamp house cover counterclockwise until the lamp house cover is loose Do not remove the screws completely from the lamp house cover ...

Страница 112: ... disconnect the connector from the lamp socket on the device 5 With your hands loosen the two screws that attach the lamp 6 Pull out the lamp 7 Align the new lamp with the locating pin When the lamp is in the correct position push the lamp gently into the lamp house The lamp socket must be in a level position with the lamp house 8 When the lamp is properly seated tighten the two screws to attach t...

Страница 113: ...tor in the Instrument Configuration Manager b Select Properties on the menu c On the Detector page enable the VIS Lamp check box 14 After replacing the lamp increased noise and strong baseline fluctuations may occur Before beginning an analysis or performing a wavelength validation allow the new lamp to run until the noise is reduced and the baseline is stable which typically takes 24 hours 15 Per...

Страница 114: ... the Flow Cell page 116 3 Install the plugs on the flow cell inlet IN and outlet OUT and place the flow cell in its packaging To exchange the flow cell follow these steps 1 Flush the flow cell with isopropanol 2 Remove the flow cell from the detector See Removing the Flow Cell page 116 3 Install a new flow cell without connection unit to the detector See Installing a Flow Cell without Connection U...

Страница 115: ...pening of the device On the rear side of the flow cell the contact pads for the identification chip are located Never touch the contact pads Avoid damage to the electronics of the ID chip Particulate matter dust and debris can lead to contamination and clogging of the flow cell If capillaries are removed from the flow cell inlet and outlet install protective plugs to close the ports and to prevent...

Страница 116: ...d using the optional flushing and injection kit 3 Flush the flow cell with HPLC grade water until the solvent leaving the flow cell is neutral pH 7 4 If cleaning the flow cell does not eliminate the problem install a new flow cell See also 2 Installing a Flow Cell with Connection Unit page 60 2 Installing a Flow Cell without Connection Unit page 60 7 6 3 Removing the Flow Cell When Storing the flo...

Страница 117: ... that hold the cell in position 3 Flow cell with connection unit only Loosen the screw that holds the connection unit in position and remove the connection unit from the connection unit socket 4 Carefully pull out the cell from the opening in the detector 5 Install the flow cell plugs on the inlet and outlet ports 6 If the flow cell is to be stored Store the flow cell in its original packaging tha...

Страница 118: ...velength Detectors VC D40 VF D40 Operating Manual 7 6 4 Installing a Diagnostic Cell Parts required Diagnostic cell Follow these steps 1 Push the diagnostic cell into the flow cell opening 2 Tighten the two screws of the diagnostic cell hand tight ...

Страница 119: ... For information about the declaration see NIST Compliance page 163 A wavelength validation can also be performed with an external standard such as a caffeine solution In this case an accuracy of 1 nm can be achieved When A wavelength validation is recommended in the following situations After the flow cell has been exchanged After a lamp has been replaced Preparations Before you start the wavelen...

Страница 120: ...first few minutes after a lamp has been turned on To perform a wavelength validation 1 Turn on the flow at the flow rate that will be used for the analysis 2 In Chromeleon perform WavelengthValidation 3 If the wavelength validation fails perform a wavelength calibration from Chromeleon To perform a wavelength calibration TIP To ensure optimum performance and wavelength accuracy perform WavelengthC...

Страница 121: ... in the light path of the flow cell 2 Stop the pump flow to the flow cell Follow these steps 1 When removing the waste line from the tubing guides be careful not to pull on other tubing in the guides 2 Disconnect the waste line from the flow cell outlet or the outlet OUT of the flow cell connection unit 3 Remove the waste line from the capillary guide of the partition panel Turn the plug in the to...

Страница 122: ...es are present inside the device that could cause an electric shock or damage to the device Turn off the device with its main power switch Disconnect the power cord from both the power source and the device Use only the fuses of the type and current rating specified for the device by Thermo Fisher Scientific Do not use repaired fuses and do not short circuit the fuse holders Follow these steps The...

Страница 123: ...3 1 Use the screwdriver to remove the fuse holder 2 Replace the two fuses with new fuses of the specified type and current rating Always replace both fuses 3 Reinstall the fuse holder 4 Reconnect the power cord to the power source and to the device 5 Turn on the device with the main power switch ...

Страница 124: ...on software 3 Stop all operations on the Instrument that includes the device 4 Wait until the Instrument is idle Follow these steps 1 Start the Instrument Configuration Manager program 2 Perform a firmware update from the General tab page in the configuration dialog box for the device For details refer to the Chromeleon Help The firmware update may take several minutes NOTICE A firmware downgrade ...

Страница 125: ...whether the firmware update was successful or failed If the firmware update failed turn the device off and on again and repeat the firmware update If the firmware update fails repeatedly contact Thermo Fisher Scientific Technical Support for assistance 4 After a successful firmware update requalification of the device may be required See the release notes for a recommendation ...

Страница 126: ...door Follow these steps NOTICE To avoid damage to the door hinges be careful when performing the following sequence of steps and do not apply force 1 To remove a door push the door upward while opening Open the door to a position in which the two hinges on the housing are aligned in the grooves on the door You can remove the door only when the hinges are in the grooves Figure 27 Unhinging a door N...

Страница 127: ... door align the door with the hinges on the housing Be careful not to clamp tubing or capillaries between the door and the enclosure 4 Insert the hinges in the groove by pushing up and slightly turning the door 5 Push the door downward to lock it in place You can close the door only when it is properly installed ...

Страница 128: ...g it Use the carrying handles that were shipped with the device to move or transport the device Never move or lift the device by the front doors This will damage the doors or the device Follow these steps 1 Prepare the device for transport See Preparing the Device for Transport page 128 2 The step depends as follows To transport the device to a new location follow the instructions in Transporting ...

Страница 129: ...e in the system stack see Removing the Slide In Module page 131 See also 2 Removing the Flow Cell page 116 2 Installing a Diagnostic Cell page 118 7 12 2 Transporting the Device to a New Location Preparations Prepare the device for transport See Preparing the Device for Transport page 128 Follow these steps 1 Observe the notes for handling and lifting the device safely 2 Transport the device to th...

Страница 130: ...substances may have contaminated the device during operation and may cause personal injury to service personnel Decontaminate all parts of the device that you want to return for repair Fill in and sign the Health and Safety Form Thermo Fisher Scientific refuses to accept devices for repair if the Health and Safety Form is missing incompletely filled in or unsigned Restarting the Device after Shipp...

Страница 131: ... into the system stack or when removing it Tools required Screwdriver Torx T20 Preparations 1 Prepare the device for transport See Transporting or Shipping the Device page 128 Follow these steps 1 Loosen the four captive screws on the front left and front right of the device Figure 28 Captive screws on the slide in module 2 Push all tubing and capillaries which are present in the tubing chase of t...

Страница 132: ...ake the slide in module on both sides from below b Pull the slide in module from the rails towards the front c Place the slide in module on a clean and stable surface 7 13 2 Returning the Slide In Module Preparation If not yet done Remove the slide in module from the enclosure See Removing the Slide In Module page 131 Follow these steps 1 Install the slide in module to the enclosure of the replace...

Страница 133: ...avy Load Bulky Device The device is too heavy or bulky for one person alone to handle safely To avoid personal injury or damage to the device observe the following guidelines Physical handling of the device including lifting or moving requires a team effort of two persons A team effort is in particular required when lifting the device into the system stack or when removing it Tools required Screwd...

Страница 134: ... the slide in module on both sides from below b Lift the slide in module to the height of the enclosure c Place the slide in module in the enclosure so that the module sits in the enclosure by approximately 25 cm d Push the slide in module onto the rails and into the enclosure until the slide in module sits completely in the enclosure Figure 30 Inserting the slide in module 4 To facilitate tighten...

Страница 135: ...e slide in module in the enclosure set up and restart the device Follow these steps 1 Set up the slide in module a Connect the slide in module and set up flow connections see Installation page 45 b Prepare the slide in module for first time operation see Preparing the Device for Operation page 82 2 Prepare all other modules of the Vanquish system for operation and restart them Refer to the Operati...

Страница 136: ...7 Maintenance and Service Page 136 Variable Wavelength Detectors VC D40 VF D40 Operating Manual ...

Страница 137: ...8 Troubleshooting Variable Wavelength Detectors VC D40 VF D40 Operating Manual Page 137 8 Troubleshooting This chapter is a guide to troubleshooting issues that may arise during operation of the device ...

Страница 138: ... keypad inside provide quick visual feedback on the operational status of the device If the device firmware detects a problem the status indicators are red The problem is reported to the Chromeleon software and a message appears in the Audit Trail Alarms Leaks are a potential safety issue Therefore if a leak sensor detects leakage beeping starts to alert you in addition to the message in the Chrom...

Страница 139: ...rmware failure occurred during operation of the module an exception log has been created about the processes during the firmware failure The firmware sends the exception log to the Audit Trail when the module is connected in the Chromeleon software In this case observe the following Send the Audit Trail as cmbx file to the Technical Support before you clear the log To clear the exception log and c...

Страница 140: ...age code and turn off the module Wait for 5 seconds and turn on the module again Module malfunction detected Code xx xx Two digit to four digit code number When the message appears write down the message code Turn off the module and contact Technical Support Code 33 Leak detected eliminate within approx xx seconds xx the number of seconds within the leak must be resolved Find and eliminate the sou...

Страница 141: ...t Make sure that either the UV and or VIS lamp are on Operation can be performed only if any of the lamps or both lamps are on Code 7044 Unexpected low light intensity check lamp s for correct installation Check the lamp installation Code 7045 Unexpected high light intensity measured check flow cell installation Check if the environmental conditions ambient humidity and ambient temperature are wit...

Страница 142: ...stic Cell page 118 Perform a validation Perform a calibration and repeat the validation again If the message disappears with a different cell the flow cell that was previously installed may be clogged If the error message persists contact Technical Support Code 7057 Holmium validation failed specified wavelength not found Repeat the validation If the message still appears proceed as follows Perfor...

Страница 143: ...ts replace the flow cell Code 7130 Command rejected baseline adjustment in progress An auto zero append process is collecting data Wait until the auto zero append process is completed Repeat the auto zero command Code 7133 Acquisition aborted data rate limit exceeded The data collection rate is too high and exceeds the limits for the selected wavelength configuration In the Chromeleon software sel...

Страница 144: ...l Message and Code Description and Remedial Action Code 7143 Internal process failed no flow cell The flow cell has been removed while an internal process was running Re install a flow cell see Installing a Flow Cell page 59 It may be necessary to repeat the previous command or operation ...

Страница 145: ... previously installed Result Steps If the message disappears with a different flow cell The problems are caused by the flow cell that was previously installed If you suspect a contamination or an elevated backpressure perform a flushing procedure with the flow cell that was previously installed see Preparing the Flow Cell for Storage If the message still appears The problem is caused by the flow p...

Страница 146: ...d Cloth or tissue Preparations When resolving leakage observe the safety guidelines and general rules for maintenance and service as presented in Maintenance and Service page 99 Follow these steps 1 Locate the source of the leak Leakage usually occurs at a connection However leakage may also have occurred inside the flow cell In this case leakage may occur at the drain outlet of the flow cell see ...

Страница 147: ...re or connection from flow cell to connection unit 1 Remove the flow cell from the detector and inspect the flow cell for signs of leakage 2 If signs of leakage are present at the flow cell replace the flow cell 3 With a cloth or tissue thoroughly absorb all liquid that has collected in the leak tray and under the leak sensor Be careful not to bend the sensor 4 Allow the sensor to adjust to the am...

Страница 148: ...8 Troubleshooting Page 148 Variable Wavelength Detectors VC D40 VF D40 Operating Manual ...

Страница 149: ...le Wavelength Detectors VC D40 VF D40 Operating Manual Page 149 9 Specifications This chapter provides the physical and performance specifications including information about the materials used in the flow path of the device ...

Страница 150: ...idation Internal validation with holmium oxide filter Signal channels VC D40 Up to 2 signal channels VF D40 Up to 4 signal channels Reference channel Reference beam with same wavelength as measurement beam Data collection rates Data collection rates under Chromeleon 7 data system Other maximum data rates may be applicable under different data systems Single channel VC D40 Adjustable 0 2 Hz 125 Hz ...

Страница 151: ...g in expansion board to output absorption channels Resolution 20 bit Maximum data rate 50 Hz Outputs can be configured via software output voltage range 0 to 1 V or 0 to 10 V sensitivity and offset Control Chromeleon 7 The device can be operated also with other data systems For details contact the Thermo Fisher Scientific sales organization Keypad with 3 buttons for performing certain functions di...

Страница 152: ... Stainless steel fused silica PEEK fluoropolymers titanium No Standard Bio flow cell PEEK 10 mm 11 µL 5 MPa PEEK Fused silica Yes Semi micro flow cell SST 7 mm 2 5 µL 12 MPa Stainless steel fused silica PEEK fluoropolymers titanium No Semi micro Bio flow cell PEEK 7 mm 2 5 µL 5 MPa PEEK fused silica Yes Semi preparative flow cell 0 4 mm 0 7 μL 10 MPa PEEK fused silica Yes Nano flow cell 10 mm 3 nL...

Страница 153: ... storage temperature 20 C 45 C Ambient operating humidity 20 80 relative humidity non condensing Ambient storage humidity Maximum 60 relative humidity non condensing Operating altitude Maximum 2000 m above sea level Pollution degree 2 Power requirements Wide range 100 240 V AC 10 50 60 Hz max 245 W 255 VA Overvoltage category II Emission sound pressure level typically 61 dB A Dimensions height x w...

Страница 154: ...9 Specifications Page 154 Variable Wavelength Detectors VC D40 VF D40 Operating Manual ...

Страница 155: ...Operating Manual Page 155 10 Accessories Consumables and Replacement Parts This chapter describes the standard accessories that are shipped with the device and the accessories that are available as an option This chapter also provides information for reordering consumables and replacement parts ...

Страница 156: ...ly with the replacement parts and additional components options and peripherals specifically authorized and qualified by Thermo Fisher Scientific Accessories consumables and replacement parts are always maintained at the latest technical standard Therefore part numbers are subject to change If not otherwise stated updated parts will be compatible with the parts they replace ...

Страница 157: ...l See the content list included in the kit for the most recent information about the kit content at the time when the device is shipped Ship kit Item Quantity in shipment Partition panel plug for guiding insulated capillaries 1 System interlink cable 1 Tubing bracket 1 USB cable USB 2 0 high speed type A to type B 1 Viper capillary column compartment variable wavelength detector 1 Waste line 1 For...

Страница 158: ...0 mm stainless steel For connecting column and flow cell inlet when using UltiMate 3000 flow cells 6040 2345 There is no exact definition of the light path in capillary flow cells as is for conventional flow cells Therefore you should not use this value for calculating the absorption coefficient or the detector sensitivity according to the Lambert Beer law TIP UltiMate 3000 variable wavelength det...

Страница 159: ... including right door and left door 6083 3018 Packing material for detector 6083 0090 Fuses kit Vanquish system The kit includes the appropriate fuses for the Vanquish system modules For the device use only 3 15AT 250V AC slow blow fuses 6036 0002 Interface cables Description Part No Digital I O signaling cable 6 pin cable length 5 m 6036 0006 USB cable type A to type B high speed USB 2 0 Cable le...

Страница 160: ...les and Replacement Parts Page 160 Variable Wavelength Detectors VC D40 VF D40 Operating Manual Description Part No Power cord Japan 6000 1050 Power cord UK 6000 1020 Power cord USA 6000 1001 Power cord Switzerland 6000 1030 ...

Страница 161: ...endix Variable Wavelength Detectors VC D40 VF D40 Operating Manual Page 161 11 Appendix This chapter provides additional information about compliance UV cutoff wavelengths and the use of the digital I O ports ...

Страница 162: ...rtain hazardous substances in electrical and electronic equipment The CE mark on the device indicates that the product is compliant with the directive China RoHS regulations Measures for Administration of the Pollution Control of Electronic Information Products One of the following logos may be present on the device if applicable Logo Description The green logo marks items that do not contain the ...

Страница 163: ...U S FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instructions may cause harmful interference to radio communications Operation of this equipment in a residential area...

Страница 164: ...ge 164 Variable Wavelength Detectors VC D40 VF D40 Operating Manual The instructions were prepared in English original instructions Other language versions are translations based on the English original instructions ...

Страница 165: ...also contain salts such as sodium hydroxide NaOH Most solvents have a UV cutoff wavelength within the spectral range of the device For optimum measurement results perform quantitative measurements at a wavelength that is sufficiently above the UV cutoff wavelength The UV cutoff wavelength also depends on the quality of the solvent For information about the UV cutoff wavelength for a solvent contac...

Страница 166: ...nput Input 5 Relay output Relay_COM COM is the common contact for NO and NC If the relay is not activated or if the device is turned off the connection is between COM and NC If the relay is activated the connection is between COM and NO 6 Relay output Relay_NO Normally Open contact The next table lists the functions assigned to the connector pins and the color of the cable wire connected to each p...

Страница 167: ...ollowing prerequisites must be fulfilled The digital I O port must be connected to the external device with the digital I O signaling cable part no 6036 0006 The inputs and outputs that you want to use must be selected in the Instrument Configuration Manager Connecting a Digital I O Port 1 Plug the 6 pin connector of the cable into the digital I O port that you want to use 2 For each relay output ...

Страница 168: ...11 Appendix Page 168 Variable Wavelength Detectors VC D40 VF D40 Operating Manual ...

Страница 169: ...s 53 consumables 155 159 cTUVus mark 162 D data collection rate 88 91 92 guidelines 92 decontamination 104 105 delivery 44 detector waste line 71 deuterium lamp UV 38 107 device optimization 90 preparing for operation 82 shutdown 94 diagnostic cell 59 Dig I O 54 166 digital I O 54 166 disk space 91 door 51 open 51 remove 100 replace 126 unhinge 100 126 drain system 72 drainage 72 E equilibration 8...

Страница 170: ...5 UV lamp 38 107 VIS lamp 38 107 lamp replacement 107 deuterium lamp UV 107 tungsten lamp VIS 110 leak detection 39 88 leak sensor 146 leak sensor mode 88 leakage 146 LED bar 78 79 138 linearity 91 liquid leaks 146 long term shutdown 95 M main power fuses 122 maintenance 99 104 cleaning 104 decontaminating 104 doors 126 firmware update 124 flow cells 114 fuses 122 general rules 103 interval 104 in...

Страница 171: ... electrical safety 23 general 21 general hazards 23 installation 46 maintenance 101 operation 77 power cord 49 protective equipment 22 qualification of personnel 21 servicing 101 safety symbols 18 19 sensitivity 91 service 99 setup hardware 52 software 74 system arrangement 52 ship 128 ship kit 44 157 short term shutdown 94 shutdown 94 long term 95 short term 94 signal channel 89 signal words 18 s...

Страница 172: ...ng chase 63 tubing guides 63 tungsten lamp VIS 38 107 type label 19 U Universal Serial Bus 54 unpack 42 USB 54 UV button 79 UV cutoff wavelengths 165 UV lamp 38 89 107 turn on 85 UV_VIS 89 V Viper fitting system 67 VIS button 79 VIS lamp 38 89 107 turn on 85 W waste line 71 Wavelength 89 91 wavelength calibration 89 119 wavelength switching 91 wavelength validation 89 119 WEEE 163 ...

Страница 173: ...Index Variable Wavelength Detectors VC D40 VF D40 Operating Manual Page 173 ...

Страница 174: ...Thermo Fisher Scientific Inc 168 Third Avenue Waltham Massachusetts 02451 USA www thermofisher com ...

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