Thermo Scientific RC 3BP Plus, Инструкция по эксплуатации

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6
Control Inspection
Customer Control Inspection
6-4 Sorvall RC 3BP Plus Thermo Scientific
Temperature Controls
When verifying temperature control, all test run conditions (TEMP, SPEED, TIME, ROTOR and
option selections) should match your required protocol(s). Test fluid must be at the same volume and
temperature as the actual sample (use a calibrated thermometer to confirm). The sample temperature,
rotor assembly temperature, and SET temperature must match also. Ideally, all components would
have been stored in the same controlled-temperature area overnight. If that is not possible, you can
precool or preheat the rotor as required Either store the rotor in a controlled temperature environment
(such as refrigerator or cold room), or precool/preheat the rotor in the centrifuge chamber, until the
rotor is the same temperature as the sample and the required SET temperature.
Immediately after the test run is performed, check test fluid temperature using the same thermometer
that was used before the run.
1. Enter the program parameters. Choose the protocol with the longest run time.
2. Rotor chamber, rotor, and test fluid must have the desired sample temperature.
3. Prepare two equal loads of test fluid (similar in volume to normal run samples) equilibrated to the
desired sample temperature. Check the sample temperature using a calibrated thermometer.
4. Install the rotor in the rotor chamber.
5. Load and balance the rotor according to the instructions in the rotor manual.
6. Close the centrifuge door.
7. Press the key.
During the run, the run display temperature should be within 2° C of the set display temperature.
8. Immediately after the test run is performed, check test fluid temperature using the same
thermometer that was used before the run.
The measured sample temperature should be within 2° C of set temperature.
9. If necessary, repeat this procedure at other speeds that are commonly used in your protocols.
10. If an out-of-range temperature is observed, repeat the test run. Re-equilibrate the chamber, rotor,
and test fluid temperatures to match desired sample temperature, and repeat the test run.
Note
Test fluid must be compatible with aluminum and have a freezing point below the desired
sample temperature.