Thermo Scientific Orion AquaMate UV-Vis, Руководство пользователя

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122 | Orion AquaMate Spectrophotometer User Manual Thermo Scientific
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Standard Curve Test Menu
Concentration Measurements using the
Standard Curve Application (Custom Method)
The Standard Curve test technique is used to perform a quantitative analysis experiment using
a multipoint calibration curve. A calibration curve is composed of standards of well-known
concentration. A fit of this standard curve is used to measure the concentration of samples.
This test technique is ideal when using colorimetric reagents for which the test kit manufacturer
does not specify a factor or equation for obtaining the concentration of the test kit. Always follow
the reagent instructions provided by the test kit manufacturer when creating a custom method.
Note: If the manufacturer does not specify the wavelength for the test kit, the Scanning test
technique should be used with a prepared standard to determine the wavelength parameter for
the reagent before creating a standard curve.
Use Standard Curve Test Technique:
•
To create a standard curve by setting parameters and measuring standards for the curve
•
To view calibration curve data – select between graphical and tabular displays
•
To edit a standard curve, including changing the number of standards, selecting a different
curve fit or deleting points from the curve
•
To measure samples using the standard curve and to view and save measurement data
•
To recall existing standard curve methods
C H A P T E R 6