5
Acquiring ESI Sample Data Using the Tune Plus Window
Setting Up to Acquire MS/MS Data in the Full Scan Type
86
LTQ XL Getting Started
Thermo Electron Corporation
Figure 38.
Syringe Pump dialog box
c. If you are using a standard Unimetrics or Hamilton syringe, set up
the syringe parameters as follows:
i.
In the Type group box, click the
Unimetrics
or
Hamilton
option button to specify the proper syringe type.
ii. Click the
Volume
list box arrow to display the list of available
volumes, and then select
500
(or your syringe size) from the list
to set the proper syringe volume. Note that, if you are using a
Unimetrics syringe, the LTQ XL MS detector automatically sets
the syringe ID to its proper value of 3.260 mm.
d. If you are not using a Unimetrics or Hamilton syringe, set up the
syringe parameters as follows:
i.
In the Type group box, click the
Other
option button to make
active the syringe ID spin box.
ii. Enter the inner diameter of your syringe in the Syringe ID spin
box.
e. Click
Apply
to apply the syringe parameters and start the syringe
pump.
f. Finally, move the Syringe Pump dialog box out of the way, to the top
of the monitor screen.
7. In the Tune Plus window, observe the mass spectrum of reserpine (or
your analyte of interest). Also observe the values for NL and IT
