Thermo Scientific KingFisher Pure RNA Blood Kit, Инструкция по эксплуатации

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28 | Thermo Scientific KingFisher Pure RNA Blood Kit Thermo Fisher Scientific
Chapter 6 | General Information
Table 6-1. Available elution volumes of the KingFisher Flex and KingFisher Duo
KingFisher instrument
Elution
volumes
KingFisher Flex with 96 deep well head, elution in a KingFisher Flex 96
KF plate 50–150 µl
KingFisher Flex with 96 deep well head, elution in a Microtiter deep well
96 plate 50–1000 µl
KingFisher Flex with 24 deep well head 200–5000 µl
KingFisher Duo with 12-pin magnet head, elution in an elution strip 30−130 µl
KingFisher Duo with 12-pin magnet head, elution in a Microtiter deep
well 96 plate 50–1000 µl
To maximize the yield of purified RNA, avoid the lowest permitted volumes of
Elution Buffer in the KingFisher instruments. The Elution Buffer should cover
the KingFisher Magnetic Beads completely, and any possible sedimented
magnetic-bead pellet(s) should be completely resuspended. In addition, the
volume of the Elution Buffer should be adequate for efficient mixing of the
beads in order to obtain a maximal release of the purified RNA from the beads.
Decontamination and Disinfection of
Sample Material
You should decontaminate the sample material and the reagents and plastics
that have been in contact with the sample material in order to minimize the
risk of contamination. Use a decontaminant, such as Virkon™, paying due
attention to the manufacturer’s instructions. You should also take care of the
appropriate treatment and/or disposal of waste.