Planning sequencing runs for efficient use of consumables
Genexus
™
Integrated Sequencer consumables are designed to be used most efficiently
when samples are grouped in multiples of four. For example, running one sample
uses the same number of primer pool tubes, library and template strips, and chip
lanes as four samples. Running five or six samples requires the same consumables as
eight samples. For the most economical use of reagents, therefore, you should try to
group samples in your runs in multiples of four, if possible.
The following figures illustrate the relationship between sample number, the number
of pools in an assay, the required number of reads per sample, and the amount of deck
consumables required for a sequencing run.
1
2
1
1
1
1
2
1
One-pool assay, requiring 500K reads per sample
2
2
4
4
1
1
1
1
2
2
4
4
1
1
1
1
2
2
4
4
1
1
1
1
Two-pool assay, requiring 1M reads per sample
Appendix C
Supplemental information
Planning sequencing runs for efficient use of consumables
C
138
Genexus
™
Integrated Sequencer User Guide
