Thermo Scientific Invitrogen Countess II FL, Руководство пользователя

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3. To remove the cells counted in a specific channel from the graph, uncheck the
corresponding channel check box on the graph.
The graph automatically updates and displays the distribution of cells based on
relative fluorescence intensity only in the selected (i.e., checked) channel.
4.
To add the cells counted in a specific channel to the graph, re-check the
corresponding channel check box.
5.
To close the graph, press the Graph button again.
Gate count results
The Adjust screen for cell fluorescence assays contains the controls for gating count
results based on size, brightness, circularity, and fluorescence intensity. You can adjust
the count parameters before or after performing a count, and save these changes to
the current profile or as a separate profile (“Save as new protocol“ on page 39).
1.
On the Results screen, press More to open the Advanced
™
screen.
2.
Optional: To view your sample under a specific light source (brightfield and/or
fluorescent), select the desired Channels checkbox on the Advanced
™
screen
(“Identify cells counted in fluorescence assays“ on page 35).
3.
Optional: Press the desired boundaries buttons on the Advanced
™
screen to
identify the cells counted in the corresponding channel (“Identify cells counted
in fluorescence assays“ on page 35).
Adjust screen
Gate count results
Chapter 5 Fluorescence assays
Gate count results
5
Countess
™
II FL automated cell counter User Guide 37