Thermo Scientific iBright CL750, Руководство пользователя

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Universal Mode
Mode to image samples containing one or more signal types (chemi, fluor, and/or
visible).
Table 3 Illumination source, excitation filter, and emission filter for the
iBright
™
CL750 Instrument.
Illumination Excitation Filters Emission Filters
Green trans 490-520 nm 568-617 nm
1.
Touch Choose Channel and select from one to four different dyes or stains from
the pre-populated list.
a. To edit an existing dye, touch the dye name button. Touch Dye and change
the assigned dye to any other pre-populated dye in the fluorescent dye
list. Dyes and stains in the pre-populated dye list are categorized by most
recently used, channel and name.
b. Touch False Color and change the false color assigned to that dye.
Note: Each dye is assigned a false color to be used in the composite
overlay.
c. Touch Exposure Time and manually enter the desired exposure time.
Note: Smart Exposure is recommended (see step 2).
d. To remove this assigned dye from the channel, touch Remove Dye at the
bottom left hand corner of the screen and then Confirm .
Channel refers to the type of signal you want to capture and the imaging
conditions you want to use.
•
Chemi : Channel for measuring chemiluminescent signals.
•
Fluor [Trans] : Channel for measuring fluorescent signals that are
excited by the green transilluminator and appropriate emission filter.
•
Nucleic acid : Channel for measuring fluorescent signals in gel that are
excited by the green transilluminator and the 568-617 emission filter
(stained DNA and RNA).
•
Protein : Channel for measuring fluorescent and visible signals in gel
that are excited or illuminated by the green transilluminator and the
568-617 emission filter — stained or labeled protein.
•
Visible : Channel for measuring visible signals on an opaque media
(nitrocellulose membrane, PVDF membrane, etc.) using the Epi-white
LED with neutral density filter and appropriate emission filter.
• TPN : Total protein normalization channel is for normalizing Western
blot results to total protein load by lane.
Operate the iBright
™
imager
Universal Mode
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iBright ™ CL750 Imaging System User Guide