Protein Gels
•
Protein Gels Visible:
USE WHITE SCREEN BETWEEN SAMPLE AND
TRANSILLUMINATOR GLASS for samples including Coomassie-stained protein
gels and silver-stained protein gels.
•
Protein Gels Fluorescent:
PLACE SAMPLE DIRECTLY ON
TRANSILLUMINATOR GLASS for samples including No-Stain
™
-lableled protein
gels and SYPRO
™
dye-stained protein gels.
Image using Protein Gels
1.
Select whether you are imaging a visible sample (
Protein Gel Visible
) or
fluorescent sample (
Protein Gel Fluorescent
).
2.
An auto-zoomed and focused
Live View
of the image is now displayed in the
viewport.
IMPORTANT!
For visual stained protein on nitrocellulose and PVDF
membrane, use
Universal Mode
4
Visible channel
without the white screen.
3.
Touch
Smart Exposure
. Alternatively, to set a manual exposure time, touch the
center of the dial.
The imager acquires a series of short exposures and then renders a preview
image and a recommended exposure time. This is not a real image.
4.
Touch
More options
4
Region of Interest
.
Region of Interest
is for directing
Smart Exposure
to a specific region of the sample. You can select different,
same, or no region of interest for each channel.
5.
To adjust or set a manual exposure time, select a method:
• Touch or within dial.
• Finger swipe in the segmented dial.
• Touch the dial center box to select a preset exposure time or touch
Custom
to manually input a time.
The image preview will update in real-time.
6.
Touch
Capture
to acquire image with the indicated exposure time.
Captured image appears on screen and automatically saves to the gallery.
7.
If an acceptable image, touch
Export
,
Gallery
or
Analyze
. If an unacceptable
image, touch
More options
4
Image adjust
to optimize image or touch
Trash
to
remove image, then return to step 2 to adjust exposure conditions.
Operate the iBright
™
imager
Protein Gels
iBright
™
CL750 Imaging System User Guide
15
