Thermo Scientific HM525 NX Operator Guide
59
Chapter 4 – Troubleshooting
Troubleshooting Tables
Problem
Cause
Removal
HM525NX temperature
cannot be achieved
Ambient temperature too high
Lower ambient temperature by fresh air, climate.
Please note the specified temperature of +20°C!
HM525NX is influenced by near,
heat-generating instruments
Change site of installation
Draught into the HM525NX
Change site of installation
Soiled condenser
Open the service door on the left side, clean the
condenser
Defective cooling
Call a service technician
Frost built-up on
chamber wall and on
microtome
Draught (open doors, windows)
Change site of installation; close windows, doors
Retarded freezing-on
of the specimen
Surface of the fast freezing station
is soiled
Remove debris
Lower part of the chuck is soiled
or damaged
Remove debris, remove damage
Static electricity in chamber.
Chatter while cutting
Unfavourable clearance
angle
Readjust clearance angle
Sections tear or crack
Insufficient knife sharpness
Move the knife in the knife carrier
Knife not adequately clamped
Check knife clamping
Chuck inadequately clamped
Specimen inadequately frozen on
onto the chuck
Specimen grossed too thick – it
loosens from the chuck
Specimen very hard and uneven
Check clamping
Re-freeze the specimen onto chuck
Again freeze on the specimen
Select new section thickness, reduce the
specimen