Thermo Scientific HM525 NX Operator Guide
58
How to Avoid Malfunctions
To cut usable sections, the following points are of utmost importance:
•
Fit the knife edge, probably move it horizontally to the left or right.
•
Check adjustment of anti-roll plate guide and correct it, if necessary.
•
Carefully remove dirt from the front and rear part of the knife and from the anti-roll plate with
the use of ethanol, acetone or a similar reagent.
•
Tighten all clamping screws and clamping levers on the knife carrier and specimen holder.
•
Select the appropriate cutting temperature according to the specimen.
•
Select the appropriate freezing medium.
Note
If the specimen was frozen with liquid nitrogen or similar freezing techniques, the specimen must adapt itself
to the cutting temperature.
•
Carefully select the required knife material and profile.
•
Adjustment of knife height.
•
Adjustment of proper clearance angle. Usable cuts are only achieved at a clearance angle of 8-12°
or more.
•
Take care in bringing the specimen and knife together.
Remove the specimens from the cooling chamber during defrosting, as the temperature
inside the chamber will increase. Do not leave or store tissue inside the HM525 NX over
a longer period. Due to power failure or other unexpected malfunctions of the
instrument, the specimen might be damaged.
