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Thermo Scientific Dionex Easion Скачать руководство пользователя страница 44

Dionex Easion Operator’s Manual

36

Doc. 155028-01 08/20

•

Analyze samples containing nitrite or sulfite as soon as possible.
Nitrite oxidizes to nitrate, and sulfite to sulfate, thus increasing the
measured concentrations of these ions in the sample. In general,
samples that do not contain nitrite or sulfite can be refrigerated for at
least one week with no significant changes in anion concentrations.

3.10.2 Pretreating

•

Analyze rainwater, drinking water, and air particulate leach solutions
directly with no sample preparation (other than filtering and possibly
diluting).

•

Filter groundwater and wastewater samples through 0.45



m filters

before injection, unless samples were filtered after collection.

•

A Thermo Scientific

™

Dionex

™

High-Pressure Inline Filter

(P/N 044105) is available for removing particulates down to
0.45 micron from samples. Connect the inline filter between the
Dionex AS-DV outlet and the sample inlet port on the injection valve.
For more information, refer to

High-Pressure Inline Filter

Installation Instructions

(Document No. 032319).

•

Before injection, pretreat samples that may contain high
concentrations of interfering substances by putting them through
Thermo Scientific

™

Dionex

™

OnGuard

™

II cartridges. For more

information, refer to the cartridge manual.

•

If a Dionex AS-DV is installed, you may want to use Thermo
Scientific

™

Dionex

™

Guardcap

™

vial caps, which contain Dionex

OnGuard resin and allow for automated sample preparation. For more
information, refer to the vial cap manual.

3.10.3 Diluting

•

Because the concentrations of ionic species in different samples can
vary widely from sample to sample, no single dilution factor can be
recommended for all samples of one type. In some cases (for
example, many water samples), concentrations are so low that
dilution is not necessary.

•

Use eluent or ASTM Type I (18.2 megohm-cm) filtered and
deionized water to dilute the sample. When using carbonate eluents,

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Содержание Dionex Easion

Страница 1: ...Dionex Easion Ion Chromatography System Operator s Manual Document No 155028 Revision 01 August 2020 ...

Страница 2: ...ific Inc The contents of this document are subject to change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free and assumes no responsi...

Страница 3: ... Symbols 6 1 4 Regulatory Compliance 6 1 4 1 Notice on Lifting and Handling of Thermo Scientific Instruments 7 1 4 2 Notice on the Proper Use of Thermo Scientific Instruments 7 1 4 3 Notice on the Susceptibility to Electromagnetic Transmission 7 1 4 4 WEEE Compliance 7 2 Description 9 2 1 Operating Features 9 2 1 1 Front Door and Top Cover 9 2 1 2 Component Mounting Panel 11 2 1 3 Rear Panel 13 2 ...

Страница 4: ...Stabilizer 22 2 4 Chromeleon Software 23 2 4 1 The Chromeleon ePanel Set 23 2 4 2 Software Control Modes 24 2 4 3 System Wellness 25 3 Operation and Maintenance 27 3 1 Operation Overview 27 3 2 Turning On the Dionex Easion Power 28 3 3 Connecting to Chromeleon 28 3 4 Preparing the Eluent 28 3 5 Preparing the Regenerant 29 3 6 Priming the Pump 31 3 7 Equilibrating the System 34 3 8 Verifying Operat...

Страница 5: ...with a Syringe 42 3 11 6 Example Chromeleon Commands for Loading and Injecting Samples 44 3 12 Maintenance 45 4 Troubleshooting 47 4 1 Alarms and Error Conditions 47 4 2 Liquid Leaks 52 4 3 Pump Difficult to Prime or Loses Prime 53 4 4 Pump Does Not Start 53 4 5 No Flow 54 4 6 Erratic Flow Pressure Reading 54 4 7 Excessive System Backpressure 54 4 8 Peak Ghosting 55 4 9 Nonreproducible Peak Height...

Страница 6: ...ittings 66 5 3 Isolating a Restriction in the Liquid Plumbing 67 5 4 Cleaning Eluent Bottles 69 5 5 Changing the Sample Loop 69 5 6 Replacing Pump Check Valves 70 5 7 Cleaning Pump Check Valves 72 5 8 Replacing a Pump Piston Seal and Backup Seal 73 5 9 Replacing a Pump Piston 79 5 10 Replacing the Waste Valve Seal 80 5 11 Rebuilding the Injection Valve 81 5 12 Replacing the Conductivity Cell 81 5 ...

Страница 7: ...ing Information 91 C TTL and Relay Control 93 C 1 Connecting a TTL or Relay 94 C 2 Selecting TTL Input Control Modes and Functions 95 C 3 Configuring Relay Output 1 or 2 to Respond to the Pump Flow 97 C 4 Controlling TTL and Relay Outputs 97 D FAQ 99 D 1 How do I connect to the Dionex AS DV 99 D 2 Why are the retention times moving 99 D 3 How do I adjust retention times 99 D 4 When should I remake...

Страница 8: ...1 08 20 D 6 How do I shut off the system 100 D 7 How do I store columns 100 D 8 How do I know when a column is dirty 100 D 9 How do I clean a column 101 D 10 Why is the conductivity high 101 E Introduction to Ion Chromatography IC 103 F Glossary 105 ...

Страница 9: ...th a PC personal computer running Windows 10 or Windows 8 1 operating system and the Thermo Scientific Dionex Chromeleon 7 Chromatography Data System version 7 2 10 MUa and later Chromeleon provides complete instrument control data acquisition and data management For communication between the Dionex Easion and the PC on which Chromeleon is installed the Dionex Easion must be connected to a USB Uni...

Страница 10: ...n this manual including safety related information Chapter 2 Description Describes Dionex Easion operating features the chromatographic flow path and the software required for Dionex Easion control Chapter 3 Operation and Maintenance Provides operating instructions for the Dionex Easion and describes routine preventive maintenance procedures Chapter 4 Troubleshooting Lists problems and presents st...

Страница 11: ...ing Thermo Scientific Dionex CRS 500 Product Manual Document No 031727 Thermo Scientific Dionex OnGuard II Cartridges Product Manual Document No 031688 Thermo Scientific Dionex Guardcap Product Manual Document No 065705 Chromeleon 7 Installation Guide Document No 7229 0003 Appendix C TTL and Relay Control Describes the Dionex Easion TTL and relay control features Appendix D FAQ Provides answers to...

Страница 12: ...nd select option 2 Outside the U S and Canada call the nearest Thermo Fisher Scientific office 1 3 1 Safety Messages and Notes This manual contains warnings and precautionary statements that can prevent personal injury and or damage to the Dionex Easion when properly followed Safety messages appear in bold type and are accompanied by icons as shown below Indicates an imminently hazardous situation...

Страница 13: ...vitée pourrait entraîner des blessures graves à mortelles Signale une situation de danger potentiel qui si elle n est pas évitée pourrait entraîner des blessures mineures à modérées Également utilisé pour signaler une situation ou une pratique qui pourrait gravement endommager l instrument mais qui n entraînera pas de blessures Bedeutet unmittelbare Gefahr Mißachtung kann zum Tod oder schwerwiegen...

Страница 14: ...f these requirements and directives Changes to your system include replacing a part or adding components options or peripherals not specifically authorized and qualified by Thermo Fisher Scientific To ensure continued compliance with these requirements and directives replacement parts and additional components options and peripherals must be ordered from Thermo Fisher Scientific or one of its auth...

Страница 15: ...tions on the proper use of the instrument include changes to the system and parts replacement Accordingly order replacement parts from Thermo Fisher Scientific or one of its authorized representatives 1 4 3 Notice on the Susceptibility to Electromagnetic Transmission Your instrument is designed to work in a controlled electromagnetic environment Do not use radio frequency transmitters such as mobi...

Страница 16: ...ment ne doit pas être jeté avec les déchets ordinaires mais doit être collecté séparément et traité conformément aux règlementations locales et nationales WEEE Konformität Dieses Produkt entspricht der EU Waste Electrical Electronic Equipment WEEE Richtlinie 2012 19 EU Es ist mit dem folgenden Symbol gekennzeichnet Instrumente mit diesem Zeichen sind nicht für den normallen Abfall bestimmt Entsorg...

Страница 17: ...2 Description 2 1 Operating Features 2 1 1 Front Door and Top Cover Figure 2 1 illustrates the front door and top cover of the Dionex Easion Figure 2 1 Dionex Easion Eluent Bottle Regenerant Bottle Injection Port Status LEDs ...

Страница 18: ...t For more information about sample injection see Section 3 11 5 Eluent and Regenerant Bottles The Dionex Easion top cover is molded to hold one eluent bottle assembly P N 062510 and one regenerant bottle assembly for anion analyses P N 068222 for cation analyses P N 057713 Eluent carries the sample through the Dionex Easion and facilitates the ion separation process The type of eluent used depend...

Страница 19: ... configured for cation analyses uses tetrabutylammonium hydroxide TBAOH regenerant For more information about suppressor regeneration see Section 2 3 5 2 1 2 Component Mounting Panel Figure 2 2 shows the components installed on the component panel behind the Dionex Easion front door Figure 2 2 Dionex Easion Component Mounting Panel Suppressor Injection Valve Pressure Transducer Pump Heads 1 2 3 4 ...

Страница 20: ...ionex Easion For more information about suppressors see Section 2 3 4 Separator and Guard Columns Both the separator and guard columns are packed with resin and perform the separation of the sample ions The main function of the guard column is to trap contaminants and particulates that might damage the separator column Pressure Transducer The pressure transducer measures the system backpressure Fo...

Страница 21: ... Dionex Easion to the PC on which Chromeleon software is installed For the standard system configuration of one Dionex Easion connected to the PC connect a USB cable between the USB connector and a USB port on the PC For more information refer to the Dionex Easion installation instructions Figure 2 3 Dionex Easion Rear Panel Link LED Fuse Holder Power Switch and Power Receptacle Plumbing and Waste...

Страница 22: ...uses see Section 5 14 The power switch provides on off control of power to the Dionex Easion The power cord plugs into the IEC 320 three prong receptacle LED Status Description On Although the Dionex Easion and the PC are linked no data is currently being transmitted or received Flashing The Dionex Easion and the PC are linked and data is being transmitted Off The Dionex Easion and the PC are not ...

Страница 23: ...028 01 08 20 15 Plumbing and Waste Lines The following lines exit the Dionex Easion through the tubing chase in the lower left corner of the rear panel Eluent Regenerant Cell outlet Waste sample regenerant and pump priming ...

Страница 24: ...Dionex Easion Operator s Manual 16 Doc 155028 01 08 20 2 2 Fluid Schematic Figure 2 4 shows the flow path through the Dionex Easion Figure 2 4 Dionex Easion Flow Schematic 1 3 2 4 5 6 7 8 9 ...

Страница 25: ...ity of the eluent and enhances the conductivity of the analytes Regenerant flows continuously through the suppressor restoring the ion exchange sites to their original state The eluent sample mixture then flows through the conductivity cell where the analytes are detected A signal is produced and sent to Chromeleon software Finally the eluent flows out of the cell and into the regenerant bottle wh...

Страница 26: ...he pump head outlet check valve Pressure readings indicate whether the pumping system is delivering smooth accurate flow You can monitor pressure readings from Chromeleon The system pressure should remain consistent no more than a 3 difference from one pressure reading to the next High and low pressure limits can be used to stop the pump flow if a limit is exceeded Pressure limits are selected in ...

Страница 27: ...9 is installed on the valve at the factory Figure 2 6 shows flow schematics for the valve In the Load position sample is loaded into the sample loop where it is held until injection Eluent flows from the pump through the valve and to the column bypassing the sample loop Sample flows from the syringe or the Dionex AS DV sample out line if installed through the valve and into the sample loop Excess ...

Страница 28: ...ppressors refer to the Dionex CRS 500 manual 2 3 5 Displacement Chemical Regeneration DCR Displacement Chemical Regeneration DCR is the process that restores the ability of the Dionex CRS 500 suppressor to suppress eluent conductivity In DCR the eluent that exits the cell is pumped into the regenerant bottle The eluent pressurizes the bottle and pushes the regenerant into the suppressor However be...

Страница 29: ...2 Description Doc 155028 01 08 20 21 Figure 2 7 Anion Displacement Chemical Regeneration DCR Figure 2 8 Cation Displacement Chemical Regeneration DCR ...

Страница 30: ... conductance of analyte ions as they pass through the cell Temperature directly affects the conductivity of a solution For example laboratory heating and air conditioning systems can cause a regular slow cycling in the baseline This in turn can affect the reproducibility of an analysis The higher the conductivity the more pronounced the effect In ion analysis the effect of temperature variation is...

Страница 31: ...0 MUa and later is used to control the Dionex Easion Chromeleon provides complete instrument control data acquisition and data management 2 4 1 The Chromeleon ePanel Set The Chromeleon ePanel Set provides access to detailed status and control functions for each system component pump detector and so on The Home tab of the ePanel Set includes system status information a signal plot and controls for ...

Страница 32: ... Two modes of software control are available direct control and programmed control With direct control you select operating parameters and commands from the Chromeleon ePanel Set Direct control commands are executed as soon as they are entered For more information see Section 3 11 2 With programmed control you create a list of control commands known as an instrument method to be executed in chrono...

Страница 33: ...System Wellness monitors the overall health of a chromatographic system It provides built in diagnostic and calibration features that help prevent unscheduled system shutdowns and assure reliable operation of system devices For more information see Section 5 1 ...

Страница 34: ...Dionex Easion Operator s Manual 26 Doc 155028 01 08 20 ...

Страница 35: ...he Dionex Easion 3 1 Operation Overview Figure 3 1 illustrates the basic steps for routine operation of the Dionex Easion Figure 3 1 Dionex Easion Operation Flow Chart Turn on the Dionex Easion power Prime the pump Equilibrate the system Process samples Prepare samples Prepare the eluent and regenerant Connect to Chromeleon ...

Страница 36: ...omeleon 7 2 In the Console click the Instruments Category Bar 3 Select the name of the instrument in which the Dionex Easion is configured Chromeleon will connect to the instrument and display the ePanel Set see Figure 2 10 3 4 Preparing the Eluent 1 Prepare the eluent For instructions refer to the column manual 2 Fill the eluent bottle with the prepared eluent 3 Insert the stopper assembly tubing...

Страница 37: ...bout 1 cm 0 4 in into the bottle 2 Rinse the regenerant bottle with deionized water 3 Fill the bottle about halfway with deionized water 4 Determine the regenerant concentration required for the application To estimate the regenerant concentration required for an eluent strength use the appropriate formula Anion Regenerant Concentration mM eluent x 2 Cation Regenerant Concentration mM eluent x 5 E...

Страница 38: ...tle and hand tighten the cap 9 Invert the bottle three or four times to disperse the concentrate 10 Verify that the liquid lines from the ELUENT and REGEN bottles are connected to the corresponding lines from the Dionex Easion see Figure 3 2 Pour les concentrés acides comme le régénérant anionique versez toujours le concentré dans de l eau désionisée et non dans le réservoir vide Gießen Sie säureh...

Страница 39: ...3 1 Verify that the ELUENT and REGEN bottles are filled the bottle caps are installed and hand tightened and the liquid lines are connected to the bottles 2 Verify that the waste lines are directed to a waste container 3 Open the waste valve on the secondary left pump head by turning the knob one half turn counterclockwise see Figure 3 3 Opening the valve directs the eluent flow path to waste and ...

Страница 40: ...l no air bubbles are exiting the pump waste line 6 Click the pump Off button on the Chromeleon ePanel 7 Close the waste valve Do not overtighten The pump is now ready for operation NOTE A convenient way to verify that the waste valve is closed is to select the pump flow rate required for your application turn on the pump and then close the valve The pressure should rise to the value expected for t...

Страница 41: ...ure 3 4 4 Open the waste valve by turning it one half turn counterclockwise 5 Draw the syringe back to begin removing air from the flow path NOTE When the line already contains liquid the syringe is difficult to draw back In this case prime with the Prime command only see Section 3 6 6 When a small amount of liquid enters the syringe remove the syringe from the luer fitting and reconnect the waste...

Страница 42: ...e Dionex Easion the system pressure will be 15 to 20 higher than the specification in the column manual If the pressure is less than expected air may be trapped in the system To release the air temporarily remove the pump fitting P on the injection valve After allowing the air to escape reconnect the fitting If the pressure is too high there may be a restriction in the system plumbing For troubles...

Страница 43: ...ex Easion Properties dialog box To cancel standby mode and return the pump to the normal flow rate either turn on the pump from the Chromeleon ePanel or start a new sequence 3 10 Preparing Samples NOTE Sample preparation can be performed while the system is equilibrating 3 10 1 Collecting and Storing Collect samples in high density polyethylene containers that have been thoroughly cleaned with AST...

Страница 44: ...e filter between the Dionex AS DV outlet and the sample inlet port on the injection valve For more information refer to High Pressure Inline Filter Installation Instructions Document No 032319 Before injection pretreat samples that may contain high concentrations of interfering substances by putting them through Thermo Scientific Dionex OnGuard II cartridges For more information refer to the cartr...

Страница 45: ... lot to prepare the calibration standards also This is most important for fluoride and chloride which elute near the water dip To improve the accuracy of early eluting peak determinations such as fluoride at concentrations below 50 ppb dilute standards in eluent or spike the samples with concentrated eluent to minimize the water dip For example spike a 100 mL sample with 1 0 mL of a 100X eluent co...

Страница 46: ...5 shows the typical steps for each type of sample processing Figure 3 5 Sample Processing Overview Manual Sample Processing Using Chromeleon Inject the sample Autozero Stop data acquisition Optional Save the manual sequence data Start data acquisition Automated Sample Processing Using Chromeleon Create a sequence Sequence Wizard Start the queue Add the sequence to the queue Load the sample ...

Страница 47: ... 11 4 or a syringe see Section 3 11 5 3 Click Autozero on the Chromeleon ePanel 4 Switch the injection valve to the Inject position with either the Dionex AS DV see Section 3 11 4 or a syringe see Section 3 11 5 The signal plot is displayed on the Chromeleon ePanel 5 Monitor the chromatogram When sample data has been collected click Monitor Baseline on the Instrument toolbar above the Chromeleon e...

Страница 48: ... instructions for evaluating the data acquired Templates for displaying chromatographic data on the screen and for printing reports There are two ways to create a sequence in Chromeleon eWorkflows provide predefined templates and rules for creating new sequences If they have been defined for your laboratory eWorkflows are the recommended method for creating a sequence The Sequence Wizard provides ...

Страница 49: ...red For more information refer to the Dionex Easion installation instructions and the autosampler manual 2 Prepare and fill sample vials and place them in the Dionex AS DV tray For more information refer to the autosampler manual 3 Use one of the following methods to load sample into the injection valve sample loop and inject it onto the column Automatically Include the Load and Inject commands in...

Страница 50: ...e front of the Dionex Easion pull method NOTE For instructions on how to load and inject samples with an autosampler see Section 3 11 4 Loading Samples with a Syringe Push Method 1 Verify that the sample loading port on the front of the Dionex Easion is connected to sample port S 5 on the injection valve see Figure 3 6 2 Fill a syringe with a calibration standard or sample 3 Insert the syringe int...

Страница 51: ... For more information about automatic sample processing see Section 3 11 3 Loading Samples with a Vacuum Syringe Pull Method 1 Verify that the sample loading port on the front of the Dionex Easion is connected to sample port S 5 on the injection valve see Figure 3 6 2 Disconnect the waste line from port W 6 on the injection valve and attach a shorter line 25 to 30 cm 10 to 12 in of PEEK or PTFE po...

Страница 52: ...ally Click Inject on the Chromeleon ePanel For more information about manual sample processing see Section 3 11 2 Automatically Include the Inject command in a Chromeleon instrument method see Section 3 11 6 For more information about automatic sample processing see Section 3 11 3 3 11 6 Example Chromeleon Commands for Loading and Injecting Samples The following example shows commands for loading ...

Страница 53: ... leaks see Section 4 2 Rinse off any dried eluent or regenerant with deionized water Check the waste container and empty when needed Weekly Check fluid lines for crimping or discoloration Relocate any pinched lines Replace damaged lines Check the back of the pump head as well as underneath the head for evidence of liquid leaks Normal friction and wear may gradually result in small liquid leaks aro...

Страница 54: ... Kit P N 088138 is available for this purpose The kit provides parts and instructions for performing the procedure If a Dionex AS DV is installed perform the recommended annual preventive maintenance procedure A Dionex AS DV Preventive Maintenance Kit P N 072678 is available for this purpose ...

Страница 55: ...Conditions If any of the following alarm conditions occurs an error message is displayed in the Chromeleon audit trail Each error message is preceded by an icon that identifies the seriousness of the underlying problem Icon Severity Level Description Warning A message is displayed in the audit trail If a run is in progress it is not interrupted Although the instrument can continue running or can b...

Страница 56: ...ness panel with the flow rate calibration instructions This automatically starts the pump and sets the flow rate to 1 mL min For flow rate calibration instructions see Section 5 1 3 Audit Trail Error Message Default Severity Level See Conductivity exceeds limit Warning page 48 Flow rate calibration error Warning page 48 Load inject valve error Error page 49 Module data buffer overflow Data may hav...

Страница 57: ...ious electronics related problems To troubleshoot Contact Technical Support for Dionex products for assistance The Dionex Easion electronics components cannot be serviced by the user This error indicates a problem in the pump controller electronics To troubleshoot Contact Technical Support for Dionex products for assistance The Dionex Easion electronics components cannot be serviced by the user Th...

Страница 58: ...ur when the eluent bottle contains less than the volume specified in the error message To troubleshoot Chromeleon determines the eluent usage by monitoring the flow rate and the length of time the pump is on After filling the eluent bottle be sure to use the Eluent Fill Volume slider on the Chromeleon ePanel to reset the volume of liquid in the bottle As the eluent is depleted the volume displayed...

Страница 59: ...oubleshooting information related to pump priming see Section 4 3 If the system pressure exceeds the maximum pressure limit for 0 5 second Chromeleon stops the pump and this error message is displayed The maximum pressure limit can be set to between 0 7 and 34 4 MPa 100 and 5000 psi in the Chromeleon Instrument Configuration Manager To troubleshoot 1 Check for blockages in the liquid lines by work...

Страница 60: ...n the pump head If they are not tighten them carefully with an open end wrench just until the leak stops Damaged pump piston seal 1 Replace the piston seal see Section 5 8 2 If the problem persists replace the piston see Section 5 9 Pump head not tight Carefully tighten the pump head mounting screws just until the leak stops DO NOT OVERTIGHTEN Leaking pressure transducer Check that the liquid line...

Страница 61: ...ction of flow 2 Make sure the plumbing downstream from the cell is clear a blockage may overpressurize the cell causing it to leak If the problem continues contact Technical Support for Dionex products for assistance 4 3 Pump Difficult to Prime or Loses Prime Empty eluent bottle and or no eluent connected 1 Fill the eluent bottle and then use the Eluent Fill Volume slider on the Chromeleon ePanel ...

Страница 62: ...priming Prime the pump see Section 3 6 Damaged piston seal Replace the piston seal see Section 5 8 Dirty pump check valve Replace see Section 5 6 or clean see Section 5 7 the check valves 4 7 Excessive System Backpressure Restriction in system plumbing Check all liquid lines for crimping or blockage Make sure the ferrule fittings are not overtightened onto tubing For more information refer to Inst...

Страница 63: ...ey may result from a contaminated malfunctioning or incorrectly installed injection valve If these peaks co elute with peaks of interest they will cause nonreproducible peak heights and or areas Insufficient time between sample injections Wait until the previous sample has been completely eluted before making another injection Insufficient flush between samples Flush the sample loop with at least ...

Страница 64: ...vity Contaminated or incorrect eluent Remake the eluent using concentrated eluent and ASTM Type I 18 2 megohm cm filtered and deionized water see Section 3 4 Contaminated or degraded sample Take appropriate precautions when preparing and storing samples to prevent contamination and degradation see Section 3 10 Contaminated column 1 Clean the column as instructed in the column manual 2 If cleaning ...

Страница 65: ...ser 4 12 High Detector Output Background not suppressed by suppressor If Conductivity exceeds limit is displayed in the Chromeleon audit trail refer to the suppressor manual for troubleshooting procedures Sample concentration too high Dilute the sample see Section 3 10 3 Wrong eluent or regenerant Check that you are using the correct eluent see Section 3 4 and regenerant see Section 3 5 for your s...

Страница 66: ... is closed Insufficient conductivity cell backpressure Verify that the correct backpressure coils are installed between the cell outlet and the regenerant bottle inlet For connection instructions refer to the Dionex Easion installation instructions Standard bore systems Use one or two of the black backpressure coils P N 045877 provided in the Dionex Easion Ship Kit P N 067768 Microbore systems Use...

Страница 67: ...erature Contact Technical Support for Dionex products for assistance Damaged piston seal Replace the piston seal see Section 5 8 DCR waste backpressure tubing not installed or installed incorrectly Verify that the DCR waste backpressure tubing is installed on the end of the suppressor waste line For more information refer to the Dionex Easion installation instructions ...

Страница 68: ...Dionex Easion Operator s Manual 60 Doc 155028 01 08 20 ...

Страница 69: ...rt refer to the troubleshooting information in Chapter 4 to correctly identify the cause of the problem 5 1 Diagnostics and Calibrations The Wellness panel in Chromeleon provides controls for performing diagnostic and calibration functions for the Dionex Easion This section provides an overview of Wellness panel functions Use Wellness panel controls to perform the following procedures Calibration ...

Страница 70: ...ectrochemical detector ECD conductivity pump Flow rate and total system Pressure are displayed View and or Reset Calibration Parameters Click the Calibration Values button to open a window that displays the current calibration values the previous values and the factory set values You can reset the current calibration values to the previous or factory set values from the window Calibration Pressure...

Страница 71: ... are a measure of the noise of the respective channels These values will vary depending on eluent pump seal age and wear background conductivity and so on The comparison of values obtained under the same conditions can be used as a relative measure of system performance The Variance Minimum and Maximum readings for the last time the Pump and Conductivity Cell diagnostic tests were run are displaye...

Страница 72: ...ick Calibrate Slope A panel with instructions and command buttons appears 8 Follow the instructions on the panel to complete the transducer slope calibration procedure The new slope will be determined and then stored as the current value 9 Turn off the pump 10 Disconnect the pressure gauge and backpressure tubing 11 Reconnect the pressure transducer to the pump Figure 5 1 Dionex Easion Interior Co...

Страница 73: ... of weighing more than 10 g with 0 001 g readability Tared beaker To calibrate 1 To open the Wellness panel click Wellness on the Chromeleon ePanel see Figure 2 10 2 Under Pump Flow Rate Calibration click Calibration Procedure A panel with instructions and command buttons appears 3 Follow the instructions on the panel to complete the pump flow rate calibration procedure NOTE Enter the weight of th...

Страница 74: ...on port to the injection valve Tubing Size and Type Used for 0 125 mm 0 005 in ID PEEK red P N 044221 Connection from pump pulse damper to pressure transducer 0 25 mm 0 010 in ID PEEK black P N 042690 Connections between all other system components 0 50 mm 0 020 in ID ETFE light blue P N 035519 Connection from injection port on door to injection valve 0 75 mm 0 030 in ID PEEK green P N 044777 Conn...

Страница 75: ...ning at the cell exit One at a time loosen each fitting and observe the pressure The connection at which the pressure drops abnormally indicates the point of restriction If the restriction has caused such high pressure that the system cannot be operated you must work forward through the flow schematic adding parts one at a time until an abnormal pressure increase and hence the restriction is found...

Страница 76: ...Dionex Easion Operator s Manual 68 Doc 155028 01 08 20 Figure 5 2 Dionex Easion Flow Schematic ...

Страница 77: ...ave left a slimy film on the bottle use an algicide or disinfectant for example dilute hydrogen peroxide 5 Rinse cleaning chemicals out of the bottle with ASTM Type I 18 2 megohm cm filtered and deionized water 6 Dry the bottle with clean particulate free air 5 5 Changing the Sample Loop A 10 L PEEK sample loop P N 042949 is installed on the injection valve at the factory Unless you are using a 2 ...

Страница 78: ... same time If new check valves are not available clean the check valves and reinstall them see Section 5 7 To remove the old check valves 1 Turn off the Dionex Easion power switch 2 Disconnect the power cord 3 Put on standard disposable laboratory rubber gloves powder free particle free and oil free before disassembling the pump head 4 Disconnect the tube fittings from the inlet and outlet check v...

Страница 79: ...single hole end of the cartridge is visible If the single hole end is not visible remove the cartridge from the housing and install it correctly Figure 5 5 Check Valve Assemblies NOTE The pump will not operate properly unless the cartridge is installed in the housing in the correct orientation Liquid flows through the check valve in the large single hole and out the small double holes Figure 5 4 P...

Страница 80: ...Cleaning Pump Check Valves If a check valve leaks or is dirty both check valves should be replaced see Section 5 6 However if new check valves are not available follow the instructions here to clean both check valves and reinstall them 1 Follow Step 1 through Step 5 in the check valve replacement procedure to remove the check valve cartridges from the valve housings 2 Place the check valve housing...

Страница 81: ...ry rubber gloves powder free particle free and oil free before disassembling the pump head 4 Disconnect all tubing connections to the pump head with the defective seal 5 Using a 7 64 in hex key P N 068227 loosen the two screws on the pump head Remove the screws and then carefully remove the head and place it on a clean surface 6 Slowly pull the pump head and allow it to separate from the housing C...

Страница 82: ...on slightly and pull the piston away from the pump Refer to Figure 5 6 or Figure 5 7 when disassembling and reassembling the pump head Vermeiden Sie Seitwärtsbewegungen wenn Sie den Pumpenkopg vom Kolben lösen Andernfalls kann der Kolben brechen Figure 5 6 Primary Pump Head Primary Pump Head P N 067702 Piston P N 052840 Seal Holder P N 067706 Backup Piston Seal P N 063382 Spacer with Drain P N 067...

Страница 83: ...clean laboratory air at the spacer to dislodge the seal holder or Insert a thin object or pointed tool into the small notch on the spacer and carefully pry out the seal holder Be careful not to scratch the spacer 3 Insert a thin object for example the straightened end of a paper clip into the spacer and carefully pry the backup piston seal out of the spacer Be careful not to scratch the spacer Fig...

Страница 84: ...e seal is correctly seated d Pull out the piston The top of the seal should be flush with the surface of the spacer e Press the seal holder onto the spacer To remove the old piston seal from the pump head 1 Fill the pump head cavity with deionized water by injecting the liquid through the piston opening 2 Reinsert the piston about 3 mm 0 125 in into the seal The backup piston seal is made of soft ...

Страница 85: ...E If the piston seal is not removed make sure the 10 32 fitting plug s are tight and add more water Make sure the pump head contains no air bubbles and then repeat Steps 2 and 4 5 Remove the 10 32 fitting plug s To install the new piston seal 1 If this is the secondary pump head open the waste valve knob by turning the knob one half turn counterclockwise 2 Lubricate the seal and the pump head open...

Страница 86: ...l push down on the spacer until it is flush with the pump head A clicking sound indicates that the seal is correctly seated 6 Remove the spacer and piston and verify that the seal is flush with the surface of the pump head 7 Verify that the O ring is installed in the spacer ...

Страница 87: ... follower picks up the piston The follower is the cylinder that holds the piston in place as it moves in and out of the pump head assembly 3 Reinstall the pump head and piston assembly Tighten the screws evenly To complete the procedure 1 Reconnect all liquid lines to the pump head 2 Close the waste valve knob 3 Turn on the Dionex Easion power switch 4 Prime the pump see Section 3 6 5 9 Replacing ...

Страница 88: ...ut of the cavity in the head 3 If the seal is removed with the valve knob in Step 2 pull the seal off the end of the knob see Figure 5 9 If the seal is not removed with the valve knob insert a thin object for example the straightened end of a paper clip into the cavity in the pump head and carefully pull out the seal Do not scratch the cavity Figure 5 9 Waste Valve Seal Replacement Figure 5 8 Wast...

Страница 89: ... Thermo Scientific Dionex parts may impair valve performance and void the product warranty 1 Click the pump Off button on the Chromeleon ePanel 2 Open the front door of the Dionex Easion 3 Disconnect each liquid line connected to the injection valve 4 Follow the instructions provided in the Injection Valve Rebuild Kit to replace the rotor seal isolation seal and stator face 5 Reconnect all liquid ...

Страница 90: ...P N 067761 into its mounting location 9 Replace the screws on the top of the cell housing and tighten 10 Attach the orange CELL OUT line from the cell to the union attached to the black CELL OUT line 11 Attach the ELUENT OUT line to the ELUENT OUT port on the suppressor 12 Close the Dionex Easion front door 13 Reconnect the power cord and turn on the Dionex Easion power Figure 5 10 Conductivity Ce...

Страница 91: ...ght until it locks into place on the mounting panel 6 Connect the four eluent and regenerant lines to the new suppressor 7 Close the Dionex Easion front door 8 Prime the pump see Section 3 6 5 14 Changing the Main Power Fuses 1 Turn off the Dionex Easion power switch 2 Disconnect the power cord HIGH VOLTAGE Disconnect the main power cord from its source and also from the rear panel of the Dionex E...

Страница 92: ...o release the fuse drawer 4 Pull the fuse drawer out of the rear panel and remove the old fuses 5 Replace the two fuses with new fast blow IEC 127 fuses rated 3 15 A P N 954745 Thermo Fisher Scientific recommends always replacing both fuses Figure 5 11 Fuse Drawer 6 Insert the fuse drawer into the rear panel and press until the drawer snaps into place 7 Reconnect the main power cord and turn on th...

Страница 93: ...er 90 W Maximum line draw 1 5 A Fuses Two fast blow IEC 127 fuses rated 3 15 A P N 954745 Dimensions Height without bottles 33 cm 13 in Height with bottles and cap tubing 60 cm 24 in Width 24 cm 9 5 in Depth 40 cm 15 75 in Weight 10 kg 22 lb Decibel Level 53 dBA Operating Temperature 10 to 35 C 50 to 95 F Humidity 5 to 95 relative humidity noncondensing ...

Страница 94: ...is communication between the Dionex Easion and Chromeleon flashes when data is being transmitted or received Type Serial dual reciprocating piston microprocessor controlled constant stroke variable speed Construction Chemically inert metal free PEEK pump heads and flow path compatible with aqueous eluents of pH 0 to 14 and reversed phase solvents Operating Pressure 0 to 35 MPa 0 to 5000 psi Flow R...

Страница 95: ... eq min Dionex ACRS 500 2 mm 37 5 eq min Dionex CCRS 500 4 mm 150 eq min Dionex CCRS 500 2 mm 37 5 eq min Void Volume Dionex ACRS 500 4 mm 50 L Dionex ACRS 500 2 mm 15 L Dionex CCRS 500 4 mm 50 L Dionex CCRS 500 2 mm 15 L Sequential Suppression for Anions With a Dionex CRS 500 and a Thermo Scientific Dionex Carbonate Removal Device The Dionex CRD 300 is designed for use with carbonate eluent based...

Страница 96: ...ty 1 Resolution 0 0047 nS cm Calibration Factory calibrated before shipment to the user calibrated in the field with memory retention and transfer Control and Data Evaluation Provided by Chromeleon software communication with the Dionex Easion is via USB Universal Serial Bus Cell Body PEEK Cell Electrodes Passivated 316 stainless steel Cell Active Volume 1 L Maximum Cell Operating Pressure 10 MPa ...

Страница 97: ...emoval of alkaline earth and transition metals surfactants or anions and neutralization of acidic or basic samples can be achieved using optional Dionex Guardcap vial caps Software Chromeleon 7 Chromatography Data System validated for use with Windows 10 or Windows 8 1 Automated Procedure Wizards Standard feature System Wellness Standard feature Virtual Column Simulator Evaluation mode Standard fe...

Страница 98: ... Conditionals Standard feature Daily Audit Trail Standard feature Sample Audit Trail Standard feature Multiple Network Control and Network Failure Protection Optional feature Storage of System Calibration Settings Factory current and previous values stored the user can reset current values to the factory or previous values Semiautomated System Qualification IQ OQ PQ procedures Customized Reporting...

Страница 99: ... 075973 Injection Valve Rebuild Kit 024305 Luer adapter fitting 1 4 28 for manual injections Reagent Bottle Assemblies 062510 Eluent bottle assembly includes stopper and cap 068222 Anion regenerant bottle assembly includes stopper and cap 057713 Cation regenerant bottle assembly includes stopper and cap 059068 O ring for eluent or regenerant bottle stopper Reagents 057559 Anion regenerant concentr...

Страница 100: ... 4 mm Chemically Regenerated Suppressor 085093 Dionex CCRS 500 2 mm Chemically Regenerated Suppressor 067761 Conductivity cell with DS5 Detection Stabilizer Miscellaneous Items 052324 Microbore Tubing Kit 960777 USB cable 1 8 m 6 ft long 954745 IEC 127 fast blow fuses rated 3 15 amps Preventive Maintenance Kits 088138 Dionex Easion Preventive Maintenance Kit 072678 Dionex AS DV Preventive Maintena...

Страница 101: ... when the pump flow is off thus ensuring that a connected device is turned off when the pump flow is off For more information see Section C 3 Figure C 1 TTL and Relay Connector Relay loads in excess of 200 mA or with included power supplies over 60 V may damage the relay drivers on the Dionex Easion s CPU board 1 2 OUT RELAY TTL OUT 1 2 TTL GND 1 2 3 4 TTL IN 1 2 Description Solid State Relay Cont...

Страница 102: ... steps to connect the TTL or relays a For each TTL or relay to be used connect an active wire red and a ground wire black to the 12 position connector plug at the appropriate pin locations For connector pin assignments see either Figure C 1 or the label on the Dionex Easion rear panel To attach a wire to the plug Strip the end of the wire if necessary insert the wire into the plug and use a screwd...

Страница 103: ... 3 C 2 Selecting TTL Input Control Modes and Functions Follow the steps below to select a control mode and function for a TTL input 1 Open the Chromeleon Instrument Configuration Manager 2 In the Dionex Easion Properties dialog box click the TTL Inputs tab 3 Double click the TTL input name or select the name and press the F2 key The Device Configuration dialog box for the selected input appears 4 ...

Страница 104: ...tive edge has no effect Inverted Edge The inverted edge mode works identically to the normal edge mode except that the positive and negative edges are reversed in function Normal Pulse In normal pulse operation the negative edge of the TTL signal is the active edge and the positive edge is ignored A pulse width of 50 ms or more is guaranteed to be detected A pulse width of 4 ms or less is guarante...

Страница 105: ...ontrol the relay output see Section C 4 5 Click OK C 4 Controlling TTL and Relay Outputs The Dionex Easion provides two TTL outputs and two relay contacts for control of functions in external devices for example a Dionex AS DV The TTL outputs are normally at 5 volts Setting a TTL output to 0 volts turns on the action in the connected device The relay outputs can be used to switch any low voltage c...

Страница 106: ... or relay output name 3 Execute the command To program control of TTL and relay outputs Include commands for control of the TTL and relay outputs in the Chromeleon instrument method You can enter the commands in the Instrument Method Wizard on the Relay and State Devices Options page or in the Instrument Method Editor ...

Страница 107: ...lumn as instructed in the column manual Note that changes in ambient temperature will have an impact on peak retention times The effect varies with the type of column installed but it is generally more pronounced for certain cation analyses The magnitude of the effect varies with the analyte and is proportional to the temperature changes that the column is exposed to D 3 How do I adjust retention ...

Страница 108: ...remake the regenerant and fill the regenerant bottle with fresh regenerant D 6 How do I shut off the system Click the pump Off button on the Chromeleon ePanel see Figure 2 10 or Turn off the power switch on the Dionex Easion rear panel see Figure 2 3 D 7 How do I store columns Columns should be stored in eluent Refer to the column manual for complete instructions D 8 How do I know when a column is...

Страница 109: ...gh Possible reasons for high conductivity include The suppressor needs regeneration Refer to the troubleshooting section of the suppressor manual The regenerant is exhausted and should be remade See Section 3 5 for instructions for how to prepare regenerant The cell is out of calibration Contact Technical Support for Dionex products for assistance ...

Страница 110: ...Dionex Easion Operator s Manual 102 Doc 155028 01 08 20 ...

Страница 111: ...d sample ions can be identified and quantitated A PC running chromatography software converts each peak in a chromatogram to a sample concentration and produces a tabulated printout of the results The IC analysis consists of four stages see Figure E 1 1 Eluent Delivery Eluent a liquid that helps to separate the sample ions carries the sample through the ion chromatography system The Dionex Easion ...

Страница 112: ...column they flow through a suppressor that selectively enhances detection of the sample ions while suppressing the conductivity of the eluent A conductivity cell monitors and measures the electrical conductance of the sample ions as they emerge from the suppressor and produces a signal based on a chemical or physical property of the analyte 4 Data Analysis The conductivity cell transmits the signa...

Страница 113: ...rough the column required to elute an analyte Capacity factor is a dimensionless measure of retention which is independent of column length or eluent flow rate It is calculated as follows Where tr retention time to retention time of unretained solute column void volume Cell Constant k A factor determined experimentally by measuring the conductance G of a standard solution of known equivalent condu...

Страница 114: ...fficiency Synonymous with Theoretical Plates Column Selectivity a Describes the relative separation of the band maxima between two adjacent peaks Selectivity can be determined by the following a t2 t0 t1 t0 Where t1 and t2 retention time of components 1 and 2 respectively t0 retention time of unretained components void volume Concentrator Column A short column used to retain and concentrate analyt...

Страница 115: ...te A measure of column efficiency which allows comparison between columns of different lengths HETP H L N Where L the column length mm N the number of theoretical plates Ion Exchange Capacity The number of active ion exchange sites in a given weight or volume of resin this is usually expressed in meq g or meq mL Ion Exchange Resin An insoluble polymer matrix containing fixed charge exchange sites ...

Страница 116: ... is proportional to the square root of efficiency N A value of R 1 5 represents baseline separation of the two peaks Retention Time The time from injection to peak maximum the basis for identification of a species in chromatographic analysis Separator Column The column used to perform a chromatographic separation also called analytical column Siemens S Unit of conductance the reciprocal of the ele...

Страница 117: ...Volume V0 The volume occupied by the eluent in a packed column This volume includes the volume between the injection valve and the column as well as between the column and the detector cell Unretained components are eluted in the void volume ...

Страница 118: ...Dionex Easion Operator s Manual 110 Doc 155028 01 08 20 ...

Страница 119: ...ore systems 58 For standard bore systems 58 Backup piston seal Replacing 73 Baseline conductivity 34 Baseline drift 57 Baseline noise 17 57 Blockages Liquid lines 51 67 Bottle Eluent 10 Regenerant 10 Bottle eluent Cleaning 69 Connecting 30 Monitoring the liquid level 28 Bottle regenerant Connecting 30 C Cables Power cord 83 USB 13 Calibration 61 Flow rate 65 Pressure transducer 63 Carbonate eluent...

Страница 120: ...lume 88 Cell body 88 Chemical compatibility 88 Electrodes 88 Maximum pressure 88 Temperature 88 Conductivity exceeds limit error 48 CRS 500 suppressor 12 20 See also Suppressor D Data collection 39 Cell temperature during 22 DCR See Displacement chemical regeneration Decibel level 85 Detection limit ranges 23 Detector signal plot 39 Detector specifications Auto offset 88 Calibration 88 Cell drive ...

Страница 121: ...e error 49 Pump pressure slope calibration error 50 Remaining eluent below 100 mL 50 Remaining eluent below 200 mL 50 System pressure below low pressure limit 51 System pressure exceeds high pressure limit 51 F Fittings Leaking 52 Replacing 66 Requirements 66 Flashing LED 10 Flow path 16 Flow rate 12 Automatically reducing 35 Calibrating 62 65 Standby mode 35 Flow rate calibration error 48 Flow ra...

Страница 122: ... 45 Daily 45 Ongoing 45 Weekly 45 Yearly 45 Manual sample processing 38 39 Methods See Instrument methods Chromeleon Microbore system Backpressure coil requirements 58 Microbore Tubing Kit 58 66 Module data buffer overflow error 49 MSA eluent 11 29 N Nitrite in samples 36 No detector response 56 Noisy baseline 57 Normal edge TTL input control 96 Normal pulse TTL input control 96 O Operating featur...

Страница 123: ...70 Flow rate range 12 Piston seal replacement 73 Primary pump head 17 Priming 31 34 Priming problems 53 Secondary pump head 18 Troubleshooting 53 Pump head waste valve Leaking 52 Seal replacement 80 Pump motor lost control error 49 Pump pressure hardware error 49 Pump pressure slope calibration error 50 Pump specifications Construction 86 Delay volume 86 Eluent bottle pressure 86 Eluent generation...

Страница 124: ...treating 36 Processing 38 Seal replacement 74 Secondary pump head 18 75 Selectivity Troubleshooting 56 Separator column 12 Sequences Chromeleon 40 Service chase 13 Service procedures 71 Backup piston seal replacement 73 Cell replacement 81 Check valve cleaning 72 Check valve replacement 70 Eluent bottle cleaning 69 Fuse replacement 83 Injection valve rebuilding 81 Piston replacement 79 Piston seal...

Страница 125: ...ariations 22 Top cover 9 Transducer 12 Troubleshooting 56 Backpressure is excessive 54 Baseline drift 57 Baseline noise 57 Cell output is high 57 Detector output is high 57 Diagnostics 61 Error messages 47 Liquid leaks 52 No cell response 56 No detector response 56 No pump flow 54 Peak ghosting 55 Peak height is nonreproducible 56 Pressure is erratic 54 Pump does not start 53 Pump flow is erratic ...

Страница 126: ... W Waranty voiding 61 Waste lines 15 Blocked 52 Waste valve 18 Opening 18 73 Seal replacement procedure 80 Water samples 36 Wellness panel Chromeleon 61 62 Audit trail 63 Diagnostics 63 Dummy cell test 63 Flow rate calibration 62 Opening 63 65 Presssure transducer calibration 62 System status 62 ...

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