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4 • Troubleshooting

Doc. 065330-05 03/2020

7. Incorrect volume
removed from the
sample container.

7a. Samples with large
amounts of suspended solids.

7a. Increase the sample volume
specified in the

Load Sample

step by 5% to 7%. For example,
specify 1050 mL instead of
1000 mL.
–or–
Filter the sample, collect the
filtrate, and place this in the
sample container.

7b. Sample pump is out of
calibration.

7b. Perform a

Clean Sample

Path

step with deionized water,

and then run the Calibrate Pump
diagnostic method (see

Section 4.2

7c. Fittings are loose at the
sample pump.

7c. Tighten the fittings.

7d. Sample pump is leaking.

7d. Clean the pump cylinder and
piston (see

Section 5.9

8. Uneven volumes in
elution containers. For
example, 10 mL is
expected, but one
container has 8 mL and
the other has 12 mL.

8. Loose fitting at 12-port
valve or switching valve.

8. Make sure valve fittings are
securely finger-tight. If
tightening a fitting does not stop
a leak, replace the fitting (see

Section 5.1

Symptom

Probable Cause

Solution

Содержание Dionex AutoTrace 280

Страница 1: ...Dionex AutoTrace 280 SPE Instrument Operator s Manual Document No 065330 Revision 05 March 2020...

Страница 2: ...in future revisions Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free Thermo Fisher Scientific Inc assumes no responsibility and will not be l...

Страница 3: ...ific Instruments 6 1 4 2 Notice on the Proper Use of Thermo Scientific Instruments 7 1 4 3 Notice on the Susceptibility to Electromagnetic Transmission 7 1 4 4 WEEE Compliance 7 2 Description 9 2 1 Op...

Страница 4: ...2 2 Creating a Method to Clean the Sample Lines 45 3 3 Application Tips 47 3 3 1 Sample Recovery 47 3 3 2 Setup Parameters 48 3 3 3 Method Steps 48 3 4 Loading a Method 50 3 5 Preparing the Dionex Aut...

Страница 5: ...g the Method 63 3 8 4 After Running the Method 63 3 9 Daily Operation Checklist 63 3 9 1 Preparing the System 63 3 9 2 Running Your Method 64 3 9 3 After Running the Method 64 3 10 Routine Maintenance...

Страница 6: ...ing a Cartridge Holder Standard Dionex AutoTrace 280 96 5 8 Converting a Cartridge Holder Dionex AutoTrace 280 PFAS 99 5 9 Cleaning a Sample Pump Cylinder and Piston 101 5 10 Calibrating a Sample Pump...

Страница 7: ...9 Sample Pumps 125 B Installation 127 B 1 Facility Requirements 127 B 2 Unpacking the Dionex AutoTrace 280 127 B 3 Installation Instructions 128 B 3 1 Connecting the Solvent Reservoirs 128 B 3 2 Insta...

Страница 8: ...Dionex AutoTrace 280 Operator s Manual vi Doc 065330 05 03 2020 C Reordering Information 145 D Glossary 151 Index...

Страница 9: ...n in this manual applies to both the standard and PFAS systems The Dionex AutoTrace 280 is available in both cartridge and disk models Cartridge models use SPE devices in the cartridge format Polymer...

Страница 10: ...e pressure ensures more uniformity when loading or eluting which leads to better reproducibility Effluent from the SPE adsorbents can be collected in six different elution containers 11 mm GC vials 15...

Страница 11: ...for assistance Chapter 1 Introduction Introduces the Dionex AutoTrace 280 explains the conventions used in this manual including safety related information Chapter 2 Description Describes Dionex Auto...

Страница 12: ...3 1 Safety Messages and Notes This manual contains warnings and precautionary statements that can prevent personal injury and or damage to the Dionex AutoTrace 280 when properly followed Safety messag...

Страница 13: ...t e pourrait entra ner des blessures graves mortelles Signale une situation de danger potentiel qui si elle n est pas vit e pourrait entra ner des blessures mineures mod r es galement utilis pour sign...

Страница 14: ...y symbols on the Dionex AutoTrace 280 model data label indicate that it is in compliance with the following EMC and safety standards Safety Specifications EN 61010 1 2010 EMC Specifications EN 61326 1...

Страница 15: ...its authorized representatives 1 4 3 Notice on the Susceptibility to Electromagnetic Transmission Your instrument is designed to work in a controlled electromagnetic environment Do not use radio frequ...

Страница 16: ...uipement ne doit pas tre jet avec les d chets ordinaires mais doit tre collect s par ment et trait conform ment aux r glementations locales et nationales WEEE Konformit t Dieses Produkt entspricht der...

Страница 17: ...e front of the Dionex AutoTrace 280 Figure 2 1 Dionex AutoTrace 280 Front View Cartridge Model Part Function 1 Sample Pumps Pumps sample from sample containers to switching valves 2 Cartridge Disk Hol...

Страница 18: ...ollects aqueous waste solvent waste or sample effluent on the elution station see Figure 2 4 4 Front Panel Communicates system status to the user LED State Meaning READY RUN ERROR On Off Off The Dione...

Страница 19: ...on is pressed which displays the START screen see Figure 2 3 Button Function LOAD Increments the method number displayed on the front panel PAUSE Pressing PAUSE while the system is idle displays the S...

Страница 20: ...ods see Section 4 2 Methods 32 through 34 are reserved for use by Thermo Fisher Scientific Manufacturing personnel 2 1 3 Cartridges The Dionex AutoTrace 280 uses silica based Thermo Scientific Dionex...

Страница 21: ...oTrace 280 Figure 2 4 Dionex AutoTrace 280 Left Side View Part Function 1 Solvent Ports Behind door not shown Input ports for five solvents 2 Waste Port Behind door not shown Output port for system wa...

Страница 22: ...8 Elution Rack Holds tubes or vials for collection of sample effluent 9 Elution Station Directs effluent to one of two waste positions or collects effluent in the collection container by moving it int...

Страница 23: ...t Function 1 Sample Input Lines Connects the system to sample containers 2 Exhaust Port Outlet for routing solvent vapors to a suitable vent location 3 USB Port Connects the USB cable from the Dionex...

Страница 24: ...r information as well as the Dionex AutoTrace 280 serial number You will be asked to provide the serial number when ordering replacement parts The power supply cord is used as the main disconnect devi...

Страница 25: ...er for position 1 only For details about the SPE process see the flow charts and diagrams in Section 2 5 Figure 2 6 Dionex AutoTrace 280 Fluid Connections Pump 1 Incoming Switching Valve 1 SPE Cartrid...

Страница 26: ...rams in this section show how the Dionex AutoTrace 280 processes a typical SPE solid phase extraction method Figure 2 7 Condition Cartridge Liquid Handling and Air Push Syringes Fill Flow Chart Start...

Страница 27: ...le 1 Switching Valve 1 SPE Cartridge 1 Aqueous Waste Solvent Waste Nitrogen Manifold Regulator Gas Air Valve Air Vent 12 Port Valve Air Syringe Liquid Handling Syringe Solvent 1 Solvent 2 Solvent 3 So...

Страница 28: ...20 Doc 065330 05 03 2020 Figure 2 9 Condition Cartridge Liquid Syringe Dispenses Solvent Flow Chart Continued 12 port valve switches to the switching valve Liquid handling syringe dispenses condition...

Страница 29: ...ispenses Solvent Diagram Solvent Air Gas Pump 1 Incoming Sample 1 SPE Cartridge 1 Aqueous Waste or Solvent Waste Nitrogen Manifold Regulator Gas Air Valve Air Vent 12 Port Valve Air Syringe Liquid Han...

Страница 30: ...Figure 2 11 Condition Cartridge Air Push Flow Chart Continued Air push syringe pushes conditioning solvent through switching valve and into cartridge See Figure 2 12 for air push with Air Factor of 1...

Страница 31: ...gram Solvent Air Gas Pump 1 Incoming Sample 1 SPE Cartridge 1 Aqueous Waste or Solvent Waste Nitrogen Manifold Regulator Gas Air Valve Air Vent 12 Port Valve Air Syringe Liquid Handling Syringe Solven...

Страница 32: ...r of 0 5 Diagram Solvent Air Gas Pump 1 Incoming Sample 1 Switching Valve 1 SPE Cartridge 1 Aqueous Waste or Solvent Waste Solvent stops here with 0 5 Air Factor Nitrogen Manifold Regulator Gas Air Va...

Страница 33: ...2 Description Doc 065330 05 03 2020 25 Figure 2 14 Load Sample Flow Chart Continued Pump turns on and pumps sample through switching valve and onto cartridge See Figure 2 15 Continued...

Страница 34: ...re 2 15 Load Sample Diagram Sample Gas Pump 1 Incoming Sample 1 Switching Valve 1 SPE Cartridge 1 Aqueous Waste Nitrogen Manifold Regulator Gas Air Valve Air Vent 12 Port Valve Air Syringe Liquid Hand...

Страница 35: ...escription Doc 065330 05 03 2020 27 Figure 2 16 Cartridge Rinse Liquid Handling and Air Push Syringes Fill Flow Chart Continued Continued Liquid handling syringe fills with rinse solvent See Figure 2...

Страница 36: ...coming Sample 1 Switching Valve 1 SPE Cartridge 1 Aqueous Waste Solvent Waste Nitrogen Manifold Regulator Gas Air Valve Air Vent 12 Port Valve Air Syringe Liquid Handling Syringe Solvent 1 Solvent 2 S...

Страница 37: ...05 03 2020 29 Figure 2 18 Cartridge Rinse Liquid Handling Syringe Dispenses Solvent Flow Chart Continued 12 port valve switches to the switching valve Liquid handling syringe dispenses rinse solvent S...

Страница 38: ...dling Syringe Dispenses Solvent Diagram Pump 1 Incoming Sample 1 Switching Valve 1 SPE Cartridge 1 Aqueous Waste Solvent Waste Nitrogen Manifold Regulator Gas Air Valve Air Vent 12 Port Valve Air Syri...

Страница 39: ...2 Description Doc 065330 05 03 2020 31 Figure 2 20 Cartridge Rinse Air Push Flow Chart Continued Air Push rinses flow path and cartridge See Figure 2 21 Continued...

Страница 40: ...dge Rinse Air Push Diagram Pump 1 Incoming Sample 1 Switching Valve 1 SPE Cartridge 1 Aqueous Waste Solvent Waste Nitrogen Manifold Regulator Gas Air Valve Air Vent 12 Port Valve Air Syringe Liquid Ha...

Страница 41: ...2 Description Doc 065330 05 03 2020 33 Figure 2 22 Dry with Gas Flow Chart Continued Switching valve selects gas port Gas flows through cartridge to dry See Figure 2 23 Continued...

Страница 42: ...3 Dry with Gas Diagram Pump 1 Incoming Sample 1 Switching Valve 1 SPE Cartridge 1 Aqueous Waste Solvent Waste Nitrogen Manifold Regulator Gas Air Valve Air Vent 12 Port Valve Air Syringe Liquid Handli...

Страница 43: ...scription Doc 065330 05 03 2020 35 Figure 2 24 Elute to Collect Liquid Handling and Air Push Syringes Fill Flow Chart Continued Continued Liquid handling syringe fills with elution solvent See Figure...

Страница 44: ...Incoming Sample 1 Switching Valve 1 SPE Cartridge 1 Aqueous Waste Solvent Waste Nitrogen Manifold Regulator Gas Air Valve Air Vent 12 Port Valve Air Syringe Liquid Handling Syringe Solvent 1 Solvent...

Страница 45: ...2 Description Doc 065330 05 03 2020 37 Figure 2 26 Elute to Collect Liquid Handling Syringe Dispenses Solvent Flow Chart Continued Continued Elution solvent is dispensed See Figure 2 27...

Страница 46: ...t Liquid Handling Syringe Dispenses Solvent Diagram Pump 1 Incoming Sample 1 Switching Valve 1 SPE Cartridge 1 Collect Nitrogen Manifold Regulator Gas Air Valve Air Vent 12 Port Valve Air Syringe Liqu...

Страница 47: ...2 Description Doc 065330 05 03 2020 39 Figure 2 28 Elute to Collect Air Push Flow Chart Continued Air Push rinses flow path and eluent goes to collection container See Figure 2 29...

Страница 48: ...2 29 Elute to Collect Air Push Diagram Pump 1 Incoming Sample 1 Switching Valve 1 SPE Cartridge 1 Collect Nitrogen Manifold Regulator Gas Air Valve Air Vent 12 Port Valve Air Syringe Liquid Handling S...

Страница 49: ...toTrace software Help 2 6 1 Standard Operations The table below describes the standard operation steps in an SPE method Operation Description Conditioning the cartridge or disk Use Condition Cartridge...

Страница 50: ...rying with gas Use Dry with Gas to flow gas through the cartridge or disk clearing the path of residual water Pausing operation Use Pausing Operation to delay operation or to stop operation and signal...

Страница 51: ...ing the Method Type Before creating any methods you must select the method type that corresponds to the type of Dionex AutoTrace 280 cartridge or disk model on which the method will run To set up the...

Страница 52: ...ame box enter a name for the method 4 In the Samples box enter the number of samples you want to process when the method runs This information is used only for estimating the time needed to run the me...

Страница 53: ...between sample runs to clean the lines Whenever the Dionex AutoTrace 280 is idle for a period of time run this method to clean the lines and leave them filled with deionized water 1 Repeat Step 1 and...

Страница 54: ...a beaker that contains Solvent for the first step Intermediate solvent for the second step if necessary Water for the last step 7 Select Dry with Gas Specify a time of 0 5 min to remove residual wate...

Страница 55: ...luate the effect of each change The remainder of this section discusses various ways in which methods can be optimized 3 3 1 Sample Recovery Table 3 1 describes three areas that can cause unacceptable...

Страница 56: ...a Cartridge Rinse or Disk Rinse after loading the sample Table 3 2 Setup Parameters If Then The sample contains volatile compounds Add a Pause and Alert step between the conditioning and loading steps...

Страница 57: ...disk after loading sample Perform a Cartridge Rinse or Disk Rinse step before elution There is water in your extract Increase the pressure and or time of the Dry with Gas step The elution solvent is n...

Страница 58: ...d are loaded into the right pane of the Set Up Methods window If Then You need to run a solvent through the sample path from sinkers through the sample lines sample pumps switching valves and cartridg...

Страница 59: ...operating setting is 0 07 MPa 10 psi 4 Check that the solvent reservoirs are full 5 Check that the tubing is fully submerged in the solvent reservoirs and that tubing weights and barbed fittings are...

Страница 60: ...artridge upward onto the bottom of the cartridge holder s plunger b Push down on the lever until the assembly clicks into place and the holder s LED comes on see Figure 3 1 Figure 3 1 Cartridge Holder...

Страница 61: ...disks are centered and then push down on the lever until the assembly clicks into place and the holder s LED comes on see Figure 3 2 d Tighten the locking collar by screwing it as far as allowed 13 If...

Страница 62: ...iner in each rack position to be used 15 Insert the sample line with tubing weight into the appropriate container Each sample line and each rack position is numbered to correspond to the numbered samp...

Страница 63: ...NOTE When the installed system is the Dionex AutoTrace 280 disk model Disk replaces Cartridge in all messages displayed on the front panel The display also reports when manual intervention is require...

Страница 64: ...n processing was interrupted not at step 1 of the method 3 6 2 Resuming a Run To resume running a method after pressing PAUSE press CONT If you press the PAUSE button before all method steps have fini...

Страница 65: ...rocedural changes during method development Test the changes Remove the used cartridges or disks Prepare to run the next batch of samples Clean up if done for the day 3 7 1 Determining the Required Ch...

Страница 66: ...ttings as required 5 To delete a step double click its entry in the method step table 6 To add a step a In the method step table click the step above where you want to add the new step b In the comman...

Страница 67: ...es 3 7 4 Removing Used Cartridges Remove the used cartridges see Figure 3 5 by squeezing the holder s lever and clip together to release the cartridge from the holder Figure 3 5 Cartridge Holder Side...

Страница 68: ...r Side View 3 7 6 Preparing the Next Sample Batch Unless the last steps of the method were Clean Sample Path steps always clean the sample lines between batches of samples 1 Place an empty cartridge i...

Страница 69: ...uld attack valves or other Dionex AutoTrace 280 parts over time flush the solvent and sample lines with water or an inert soluble solution whenever the system will not be processing samples The Prime...

Страница 70: ...tion 3 7 6 3 8 Method Development Checklist 3 8 1 Specifying Your Method Run the AutoTrace software Specify the parameter and solvent information Create the method Save and print the method 3 8 2 Prep...

Страница 71: ...cleaning the sample lines see Section 3 7 6 If you are done for the day clean up by emptying the waste containers flushing the solvent lines see Section 3 7 7 and flushing the sample lines see Sectio...

Страница 72: ...nce This section describes routine maintenance procedures that users can perform All other Dionex AutoTrace 280 maintenance procedures must be performed by Thermo Fisher Scientific personnel In order...

Страница 73: ...5 3 Check overall system performance by running the Benchmark Test diagnostic method see Section 5 17 The Benchmark Test checks the operation of the front panel elution station syringes pumps and val...

Страница 74: ...em Shutdown It is not necessary to turn off the Dionex AutoTrace 280 power at the end of daily use If the Dionex AutoTrace 280 will be idle for more than 14 days flush the solvent lines see Section 3...

Страница 75: ...l the nearest Thermo Fisher Scientific office Please have this chapter at hand when talking with Technical Support personnel 4 1 Error Messages The Dionex AutoTrace 280 can identify several potential...

Страница 76: ...e due to a tubing blockage Action Replace blocked tubing with tubing of the same length and ID see Section 5 1 Cause Loose connection between the pump motor and the sensor connector Action Reseat the...

Страница 77: ...n is blocking movement of the syringe Action Find and remove the obstruction Cause Loose connection between the syringe and the Valve Distribution PC board Action Reseat the connector Cause Malfunctio...

Страница 78: ...layed 4 Press CONT to run the selected method When you want to Run this diagnostic method Initialization Initialize system components send syringes to the home position etc Diagnose the reason the sys...

Страница 79: ...Pump a When using fewer than five solvents writing your own priming method will prime solvent lines more quickly and will conserve solvent Symptom Probable Cause Solution 1 READY LED is not on 1a Pow...

Страница 80: ...4a Check that the power is on and the system is not in an abort state or a user intervention state 4b Check that the USB cable is connected correctly between the Dionex AutoTrace 280 and the PC see Se...

Страница 81: ...in wrong reservoirs 2 Insert solvent lines into the appropriate reservoirs and prime the lines 3 Syringe stalls 3a Flow rate is too high 3a Reduce the flow rate 3b Bad cartridge or disk 3b Replace the...

Страница 82: ...place the syringe see Section 5 5 5f 12 port valve is partially blocked or is out of alignment 5f Run the 12 Port Valve Test diagnostic method see Section 4 2 If the problem continues contact Technica...

Страница 83: ...a Clean Sample Path step with deionized water and then run the Calibrate Pump diagnostic method see Section 4 2 7c Fittings are loose at the sample pump 7c Tighten the fittings 7d Sample pump is leak...

Страница 84: ...er O ring 2a Packing material debris on inside walls of cartridge or disk is causing a poor seal 2a Clean cartridges or disks before use see Section 3 5 2b Dirty O ring 2b Clean the O ring with a lint...

Страница 85: ...ded solids 7a Increase the sample volume specified in the Load Sample step by 5 to 7 for example specify 1050 mL instead of 1000 mL 7b Sample pump is out of calibration 7b Perform a Clean Sample Path...

Страница 86: ...Dionex AutoTrace 280 Operator s Manual 78 Doc 065330 05 03 2020...

Страница 87: ...lacing any part see the troubleshooting information in Chapter 4 to correctly identify the cause of the problem 5 1 Replacing Tubing Replace tubing assemblies whenever tubing becomes kinked or develop...

Страница 88: ...nd brass fittings for two waste lines Dionex AutoTrace 280 Tubing Fittings Kit P N 072608 Fittings for 10 lengths of 1 40 mm 0 055 in ID PTFE tubing other than sample and waste lines Table 5 1 Tubing...

Страница 89: ...4 mL Solvent 3 2 2 m 7 ft 3 44 mL Solvent 4 2 2 m 7 ft 3 44 mL Solvent 5 2 2 m 7 ft 3 44 mL Switching valve 6 51 cm 20 in 0 023 mL Switching valve 5 51 cm 20 in 0 023 mL Switching valve 4 51 cm 20 in...

Страница 90: ...side panel to improve access to the fittings 5 Remove the tubing assembly from any tubing clips 6 Route the new tubing assembly through the same clips as the old tubing assembly 7 Install the new fit...

Страница 91: ...nitrogen manifold tighten the fittings Parts and Tools Needed 2 Phillips screwdriver Soap solution Cotton tipped swabs Tighten the Fittings 1 Turn off the Dionex AutoTrace 280 power switch and discon...

Страница 92: ...Parts Needed Lint free laboratory tissue Cartridge holder O ring see Appendix C for part numbers Replace the O Ring 1 Disengage the plunger of the leaking channel Lift the plunger up and out of the ca...

Страница 93: ...eal the inner tubing Be careful not to crimp or kink any tubing 3 Remove the tubing clamp and tubing from the top of the disk holder 4 Unscrew the bolt that secures the disk holder top assembly to the...

Страница 94: ...the top of the disk holder Be careful not to scratch the groove scratches will prevent a proper seal 2 Install the new O ring Be careful not to rip or tear the O ring 3 Reinstall the large washer and...

Страница 95: ...ct the Prime Solvents diagnostic method a Press LOAD to display the LOADING METHOD screen b Press LOAD to increment the displayed method to 29 c Press CONT once to select method 29 3 Press CONT to run...

Страница 96: ...yringe to the housing see Figure 5 2 Figure 5 2 Syringe Screws and Mounting Bracket 2 Remove the black thumbscrew from the bottom of the syringe plunger 3 Gently slide the syringe assembly out of the...

Страница 97: ...g When aligned turn the syringe clockwise to secure it in the fitting 2 Tighten the small black thumbscrew on the mounting bracket 3 Slide the syringe assembly into the housing and secure the mounting...

Страница 98: ...two full turns 3 Select the Adjust Liq Syringe diagnostic method 26 again and press CONT to run the method As the plunger moves up you should hear a clicking sound 4 Turn the thumbwheel to the right u...

Страница 99: ...g the Air Push Syringe Parts Needed Air push syringe 10 mL P N 070296 Center the Syringe Plunger Follow these steps to center the syringe plunger and prepare the syringe for removal 1 Select the Adjus...

Страница 100: ...f the syringe clamp Pivot the open end of the syringe clamp out of the way see Figure 5 5 Figure 5 5 Syringe Clamp Knurled Knobs 2 Remove the black thumbscrew from the bottom of the syringe plunger 3...

Страница 101: ...inge clockwise until secure 2 Align the grooves in the chrome end piece with the tabs in the housing 3 Gently push the syringe into place 4 Position the syringe clamp across the chrome end piece and s...

Страница 102: ...e 5 6 Syringe Knurled Thumbscrew 2 Turn the thumbwheel to the left two full turns 3 Select the Adjust Air Syringe diagnostic method 27 again and press CONT to run the method As the plunger moves up yo...

Страница 103: ...ial turn see Figure 5 7 Figure 5 7 Thumbwheel Rotation 5 Tighten the knurled thumbscrew to secure the position Complete the Procedure Run the Benchmark Test diagnostic method 30 see Section 5 17 to ve...

Страница 104: ...a Dionex AutoTrace 280 PFAS see Section 5 8 Parts Needed Obtain the appropriate plunger assembly for the new cartridge size For descriptions and part numbers see Appendix C Remove the Old Plunger Ass...

Страница 105: ...e 5 Unscrew the plunger assembly see Figure 5 8 and pull it down to remove it from the cartridge holder 6 Set the plunger assembly aside 7 Repeat Step 1 through Step 6 for each cartridge holder you ne...

Страница 106: ...h the PTFE tubing end downward over the top of the stainless tubing as shown in Figure 5 9 Figure 5 9 PTFE Tubing Installation 4 Now push the tubing clamp down until it is positioned as shown in Figur...

Страница 107: ...280 see Section 5 7 Parts and Tools Needed 3 8 in open end wrench 1 4 in open end wrench Obtain the appropriate plunger assembly for the new cartridge size For descriptions and part numbers see Append...

Страница 108: ...6 Set the plunger assembly aside 7 Repeat Step 1 through Step 6 for each cartridge holder you need to convert Figure 5 11 Plunger Assembly Dionex AutoTrace 280 PFAS Part 1 Tubing assembly including 1...

Страница 109: ...ll the coupler by tightening it against the split cone fitting with a 3 8 in open end wrench 5 Use a 3 8 in open end wrench to secure the coupler Install the PEEK nut on the tubing assembly onto the c...

Страница 110: ...not to scratch the piston scratches will prevent a proper seal 10 Place the metal retainer onto the piston followed by the white washer and the two red seals The red seals must be placed onto the pist...

Страница 111: ...edure on each sample pump that requires calibration 1 Run a single step Clean Sample Path method see Section 3 2 2 using deionized water 2 Select the Calibrate Pump diagnostic method a Press LOAD to d...

Страница 112: ...in Step 5 you would turn the adjustment screw clockwise approximately one quarter of a turn 8 If the pump needs additional adjustments press PAUSE to repeat the calibration 9 Repeat Step 2 through Ste...

Страница 113: ...click the Dry with Gas command and set the time to 0 5 min 8 To save the method click Save on the toolbar 9 On the toolbar click the down arrow next to Command and select Download Method 10 If the Set...

Страница 114: ...ump s wires to the CPU board Disconnect the pump from the board 4 Remove the two screws that secure the pump to the pump mounting bracket Set the screws aside 5 Carefully pull the pump away from the m...

Страница 115: ...lve if fluid leaks from underneath the stator Parts and Tools Needed 7 64 in hex wrench 12 port valve rotor P N 072592 Remove the Defective Rotor 1 Use the 7 64 in hex wrench to remove the three hex s...

Страница 116: ...lace 4 Replace the hex screws on the front of the valve and tighten the hex screws evenly Tighten the rotor until it bottoms out on the spacer ring The rotor is spring loaded for tension Complete the...

Страница 117: ...ect method 29 3 Press CONT to run the method 4 Turn off the Dionex AutoTrace 280 power switch and disconnect the power cord from both its source and from the Dionex AutoTrace 280 right side panel 5 Op...

Страница 118: ...he last screw Install the Bracket on the New Valve 1 Remove the bracket from the motor on the defective valve by unscrewing the 3 mm hex screws 2 Install the bracket onto the new valve using the 3 mm...

Страница 119: ...ethod 30 see Section 5 17 to verify that the new valve works properly Tubing Fitting Label Instrument Panel Label Valve Stator Label V1 1 left side 2 V2 2 left side 3 V3 3 left side 4 V4 4 left side 5...

Страница 120: ...wdriver remove the six Phillips screws securing the rear panel to the system Set the panel and screws aside 3 Remove the three white compression fittings on the air push valve see Figure 5 15 Figure 5...

Страница 121: ...sen the screw on the clamp that holds the valve 6 Remove the defective valve from the system Install the New Valve 1 Install the new air push valve aligning the post with the notch on the panel see Fi...

Страница 122: ...n the power 7 Place solvent lines in the solvent reservoirs 8 Run the Prime Solvents diagnostic method 29 Complete the Procedure Run the Benchmark Test diagnostic method 30 see Section 5 17 to verify...

Страница 123: ...x Phillips screws securing the rear panel to the system Set the panel and screws aside 6 Locate the connector for the defective valve on the Valve Distribution PC board see Figure 5 19 Unplug the conn...

Страница 124: ...e the valve from the Dionex AutoTrace 280 12 Remove the two screws in the valve to separate the valve from its bracket Install the New Valve 1 Secure the new valve to the mounting bracket with the two...

Страница 125: ...removed 11 Reconnect the power cord and turn on the power 12 Reconnect the gas supply to the instrument 13 Place solvent lines in the solvent reservoirs 14 Run the Prime Solvents diagnostic method 29...

Страница 126: ...the method 3 As the method runs follow the on screen instructions to verify that the Front panel RUN LED turns on Front panel ERROR LED turns on Elution shuttle moves to its three different positions...

Страница 127: ...t the Benchmark Test 30 again and press CONT to run the method 5 18 Aligning the Elution Nozzles When the 40 mL elution rack P N 072688 and 40 mL collection vials P N 048783 are installed manually adj...

Страница 128: ...f the vial use a small pair of pliers to adjust the nozzle toward the center of the vial Figure 5 21 shows an example of a nozzle that is aligned Figure 5 21 Example of Properly Aligned Elution Nozzle...

Страница 129: ...a small screwdriver press the tab in and then up to release the fuse drawer 3 Pull the fuse drawer out of the right side panel and remove the old fuses Thermo Fisher Scientific recommends always repl...

Страница 130: ...into the springs in the fuse drawer Press gently to fully insert the fuses into the drawer 5 Insert the fuse drawer into the right side panel and press until the drawer snaps into place 6 Reconnect th...

Страница 131: ...ypical Input Power 100 W Maximum Line Draw 1 2 A Fuse Requirements Two fast blow IEC 127 fuses P N 954745 rated 3 15 A Operating Temperature 10 to 40 C 50 to 104 F Note The Dionex AutoTrace 280 is int...

Страница 132: ...commands and selecting methods Air Push Syringe 10 mL Liquid Handling Syringe 10 mL 12 Port Valve Rotary Air Push Valve 3 way PTFE Switching Valves 3 input ports to 1 output port pump input port norma...

Страница 133: ...x 100 mm stainless steel Elution Racks 40 mL collection vial Displacement Positive Accuracy 2 5 Tube Fitting Kynar Piston and Liner Ceramic Acetone is harmful to the sample pumps and should never be...

Страница 134: ...Dionex AutoTrace 280 Operator s Manual 126 Doc 065330 05 03 2020...

Страница 135: ...16 6 in x 33 02 cm 13 0 in B 2 Unpacking the Dionex AutoTrace 280 1 Open the Dionex AutoTrace 280 shipping container Remove the Dionex AutoTrace 280 and place it on the workbench 2 Unpack the Ship Kit...

Страница 136: ...oTrace software NOTE For installation instructions for the sample reservoirs see page 53 B 3 1 Connecting the Solvent Reservoirs This section provides instructions for how to connect the solvent reser...

Страница 137: ...gh the hole in one of the reservoir caps Cut the tubing length if necessary to avoid slack or kinks in the solvent lines 5 Push the tubing end through the small hole in the top of one of the tubing we...

Страница 138: ...ed Fitting 7 Gently pull the tubing upward until the barbed fitting seats itself within the recess of the tubing weight see Figure B 3 Figure B 3 Pulling Tubing Upward To hold the tubing in your finge...

Страница 139: ...e system to the solvent reservoirs 1 Position the reservoirs to the left of the Dionex AutoTrace 280 PFAS keeping the following considerations in mind To prevent contamination wear cleanroom gloves li...

Страница 140: ...and a ferrule on one end of the tubing 6 Secure the 1 4 28 PEEK nut at the end of the tubing to the tubing weight Figure B 5 Assembly of Tubing Weight and Line Dionex AutoTrace 280 PFAS 7 Cap the bot...

Страница 141: ...rain and tubing B 3 2 Installing the Waste Drain and Tubing To install and orient the waste drain and connect the solvent and waste tubing 1 Locate and remove the waste drain P N 071362 from its shipp...

Страница 142: ...se plate see Figure B 8 Figure B 8 Ball Stud and Base Plate 4 Push the waste drain toward the back of the system it moves only about 3 mm 1 8 in This correctly positions the drain under the manifold 5...

Страница 143: ...elow the level of the waste container cap b Connect the cap of the aqueous waste tubing the tubing connected to the rear drain trough to the remaining waste container making sure the waste tubing neve...

Страница 144: ...left side of the system see Figure B 10 Figure B 10 Waste Tubing Port 9 Insert the waste tubing from this port into one of the bottles supplied with the system keeping the following in mind Make sure...

Страница 145: ...ure B 11 Figure B 11 Exhaust Port 2 Route the hose to a suitable vent location If necessary use wire cutters or scissors to cut any excess hose B 3 4 Connecting the Gas Supply To connect the gas suppl...

Страница 146: ...38 Doc 065330 05 03 2020 Figure B 12 Barbed Fitting on Tubing 3 Connect the open end of the quick disconnect fitting see Figure B 13 to the gas inlet on the lower left side of the system see Figure B...

Страница 147: ...f no more than 0 69 MPa 100 psi 6 Before operating the Dionex AutoTrace 280 you must set the system pressure to 0 07 MPa 10 psi See Section 3 5 for instructions B 3 5 Checking the Compression Fittings...

Страница 148: ...system B 3 6 Installing the AutoTrace Software Always install the AutoTrace software before connecting the USB cable or turning on the power to the Dionex AutoTrace 280 and the PC This ensures that t...

Страница 149: ...tallation Wizard Follow the instructions on the wizard pages When the wizard is done click Finish to return to the AutoTrace software wizard 6 The AutoTrace software wizard checks the version of the H...

Страница 150: ...pens or On the toolbar click the down arrow next to Data and select Set Up Method Type 5 Select Cartridge Version or Disk Version and click OK B 3 8 Connecting to the PC While a computer is not requir...

Страница 151: ...ting the Power Cord Connect a modular power cord IEC 320 C13 from the main power receptacle on the Dionex AutoTrace 280 right side panel to a grounded single phase power source of 100 to 240 Vac 47 63...

Страница 152: ...itu e install e pr s du module et facilement accessible STROMSCHLAGGEFAHR Zur Vermeidung von elektrischen Schl gen ist eine geerdete Steckdose zu verwenden Das Ger t darf nicht ohne Erdung betrieben b...

Страница 153: ...SAX SPE Cartridges 088105 Dionex SolEx SAX 3 mL cartridge with 60 mg of resin Pkg of 48 088106 Dionex SolEx SAX 3 mL cartridge with 150 mg of resin Pkg of 48 088107 Dionex SolEx SAX 6 mL cartridge wi...

Страница 154: ...with 60 mg of resin Pkg of 48 088118 Dionex SolEx WCX 3 mL cartridge with 150 mg of resin Pkg of 48 088119 Dionex SolEx WCX 6 mL cartridge with 100 mg of resin Pkg of 48 088121 Dionex SolEx WCX 6 mL c...

Страница 155: ...a 6 mL cartridge with 0 5 g of resin Pkg of 48 Carbon Based SPE Cartridges 075898 Dionex SolEx Graphitized Carbon 6 mL cartridge with 0 5 g of resin Pkg of 30 074590 Dionex SolEx Activated Carbon Char...

Страница 156: ...ringe 10 mL 1 Sample and Solvent Reservoirs 048784 Sample vials glass 60 mL includes lids Pkg of 72 056284 Sample reservoirs glass 250 mL includes lids Pkg of 12 045900 Solvent or sample reservoir gla...

Страница 157: ...Pumps 071529 Sample pump for Dionex AutoTrace 280 cartridge model 1 071530 Sample pump for Dionex AutoTrace 280 disk model 1 Valves 071329 12 port valve and motor 1 072592 12 port valve rotor 1 07259...

Страница 158: ...Dionex AutoTrace 280 Operator s Manual 150 Doc 065330 05 03 2020...

Страница 159: ...5 is recommended Air Push A flow of air generated by the air push syringe that clears liquid from the liquid handling syringe and tubing lines after conditioning rinsing eluting and syringe washing st...

Страница 160: ...utoTrace 280 Operator s Manual 152 Doc 065330 05 03 2020 Push Delay The duration in seconds of the pause after liquid has been sent through the cartridge or disk This delay allows the pressure to equi...

Страница 161: ...chematic 17 Front panel controls 10 Installing 128 Left side view 13 Lifting safely 127 Model data label 16 Overview 1 Physical specifications 123 Preventive Maintenance Kits 65 Regulatory compliance...

Страница 162: ...ing Syringe Dispenses Solvent 29 Cartridges 12 Removing packing debris 52 Removing used cartridges 59 Caution icon 4 Compression fittings Checking tightness 139 Computer connection 142 D Daily mainten...

Страница 163: ...Displaying 55 Elute to Collect 2nd Tube 55 Pause and Alert 56 Rinse Sample Container 55 Fuse holder 16 Fuses 16 123 Replacing 121 G Gas inlet 13 Gas pressure gauge 14 Gas pressure operating setting 5...

Страница 164: ...nance 65 Post method tasks 57 Power cord installation 143 Power requirements 123 Power switch 16 Preventive Maintenance Kits 65 Prime Solvents diagnostic method 51 71 Push delay Definition 152 R READY...

Страница 165: ...Priming 51 71 Tubing weights 51 54 Solvent ports 13 Solvent reservoir connections 128 131 Solvent selection 41 Solvent waste tubing 14 Installing 135 Spare parts 145 SPE process 18 Specifications Coll...

Страница 166: ...ong solvent dispensed 73 Wrong volume from sample container 77 Tubing connections Replacing 79 Tubing Kits 80 U USB cable 149 Installation 143 USB port 3 15 V Valve See 12 port valve See Air push valv...

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