Thaw, vortex, and centrifuge a calibration plate
IMPORTANT!
Keep calibration plates protected from light until you perform the calibration. Do not
remove the plate from its packaging until you are ready to use it. Prolonged exposure to light can
diminish the fluorescence of the dyes in the wells of calibration plates.
1.
Remove the calibration plate from the freezer, then thaw the plate in its packaging for 30 minutes.
IMPORTANT!
Use each plate within 2 hours of thawing.
2.
While wearing powder‐free gloves, remove the calibration plate from its packaging. Do not remove
the optical film.
Note:
Do not discard the packaging for the calibration plate. Each calibration plate can be used
up to three time if the following conditions are met:
·
The plate is stored in its packing sleeve at –25°C to –15°C.
·
The plate is used within 6 months after opening.
·
The plate is used before the plate expiry date.
3.
Vortex the plate for 5 seconds, then centrifuge at 750–1,000 ×
g
for 2 minutes.
4.
Confirm that the liquid in each well is at the bottom of the well and free of bubbles. If it is not,
centrifuge the plate again.
IMPORTANT!
Keep the bottom of the plate clean. Fluids and other contaminants on the bottom
of the plate can contaminate the sample block and cause an abnormally high background signal.
Perform calibrations
1.
In the home screen tap
(Settings)
4
Maintenance and service
4
Calibration
.
The
Calibration
screen is displayed.
2.
In the
Calibration
screen, select a calibration type.
Calibration type
Tap
ROI/uniformity
ROI and uniformity
Background
Background
Dye
System dye
Chapter 7
Calibrate and verify instrument performance
Perform ROI/uniformity, background, and dye calibrations for plate blocks
7
QuantStudio
™
6 Pro Real-Time PCR System and QuantStudio
™
7 Pro Real-Time PCR System User Guide
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