Thermo Scientific A44769, Руководство пользователя

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Note:
· The valve volumes are not the same as the volume that has entered or left a bag. In the example
shown, the input bag (cells in culture) is a dual port bag connected to both valve D and G. To
calculate the volume change in or out of the input bag, add the valve volumes for valves D and G e.g.
- 54.6 + 24.9 = - 29.7mL (out of the input bag)
· The valve volume is the volume displaced by the pump and hence does not account for volume
retained in the Single-Use Kit during priming. For example, the net volume for valve A would over-
estimate the volume that has been delivered to the waste bag by the volume of the CFC Chamber
plus the kit tubing.
Note: The Pressor Sensors, P1 and P2, are at a low negative pressure meaning there is a slight
vacuum in the system. These will fluctuate during processing depending on the flow rate and pump
direction. A rapidly increasing Pressure Sensor value would indicate a flow restriction or blockage.
Error messages
Alarm or
message
Critical
alarm
Warning Instrument response GUI message
Safety system
error
Yes — Cut power to pump and
centrifuge, trigger instrument
stop state
Safety system error
Protocol run has stopped.
Press the stop button on
the instrument, close manual
clamps and open and close
the instrument door to reset.
Restart the protocol or select
a protocol step to start from. If
error persists, abort the run.
Protocol is
Malformed Yes — GUI error message after loading
protocol will appear Protocol is Malformed error
Note: Check pump calibration
number to ensure protocol flow
rates are within range.
Door lock error Yes — Trigger instrument stop state Door lock error
Protocol run has stopped.
Open and close the door, then
restart the protocol. If error
persists, abort the run.
Pump error Yes — Trigger instrument stop state Pump error
Protocol run has stopped.
Press the stop button to clear
the error, then restart the
protocol or select a step to
start from. If error persists,
abort the run.
Chapter 3
Basic instrument operation
Run a protocol
3
CTS ™ Rotea ™ Counterflow Centrifugation System User Guide
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