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Thermo Scientific 75006476, Инструкция по эксплуатации

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5

 Aerosol-tight Applications

Basic Principles

5-2

75006476 LH-4000W

Thermo Scientific

Basic Principles

•

Check that the sample containers are well suited for the desired centrifugation process.

Closing aerosol-tight

Rectangular Bucket 75006478

Grease the seals if necessary

,

 before closing the bucket. Use lubricant 76003500.

Flap both levers upwards.
The cap can 

be 

placed easily on the bucket.

Close the bucket aerosol-tight by flapping both levers down until you can hear them locking into 
place.

Figure 5-1. 

Rectangular Bucket with Cap

CAUTION

 

Aerosol-tight rotors and tubes may only be opened in an approved 

safety workbench when centrifuging dangerous samples.
Mind the maximum permissible load.

CAUTION

 

Be sure to check all sealings before starting any aerosol-tight applications.

CAUTION

 

If the levers are not flapped down properly can damage the bucket during 

centrifugation.
The bucket is not closed aerosol-tight if the levers are not in their locking position.

 

Never lift the bucket on the levers.

Содержание 75006476

Страница 1: ...Thermo Scientific LH 4000W Instruction Manual 50134636 b 07 2020 Visit us online to register your warranty thermofisher com labwarranty ...

Страница 2: ...WEEE Conformity This product is subject to the regulations of the EU Waste Electrical Electronic Equipment WEEE Directive 2012 19 EU It is marked by the following symbol ...

Страница 3: ... 4 Rotor Loading 4 1 Before a Run 4 2 Proper Loading 4 2 Improper Loading 4 3 Maximum Loading 4 3 Cycle Counter 4 4 Chapter 5 Aerosol tight Applications 5 1 Basic Principles 5 2 Closing aerosol tight 5 2 Fill Level 5 3 Checking the Aerosol Tightness 5 3 Chapter 6 Maintenance and Care 6 1 Cleaning Intervals 6 2 Cleaning 6 2 Disinfection 6 3 Decontamination 6 4 Autoclaving 6 5 Service of Thermo Fish...

Страница 4: ......

Страница 5: ...ll result in the expiration of the sellers warranty Scope of Supply If any parts are missing please contact your nearest Thermo Fisher Scientific representative Precautions Article Number Quantity Check 75006476 LH 4000W with Bucket 1 76003500 Grease for rubber seal 1 75003786 Bolt grease 1 50134706 CD with manual 1 This symbol refers to general hazards WARNING means that injuries or material dama...

Страница 6: ...perate the rotor always with the lid closed Use only accessories which have been approved by Thermo Fisher Scientific Exceptions to this rule are commercially available glass or plastic centrifuge tubes provided they have been approved for the speed or the RCF value of the rotor Please observe the safety instructions Please pay particular attention to the following aspects Rotor installation Check...

Страница 7: ...Thermo Scientific 75006476 LH 4000W 1 1 1 Rotor Data Contents Technical Data on page 1 2 ...

Страница 8: ... yes with cap 75006421 yes with cap 75006421 yes with cap 75006421 Permissible temperature range autoclavable C 121 121 121 Table 1 2 230 V 50 60 Hz Double Rectangular Bucket DoubleSpin 2 x 250 ml 75006478 Centrifuge Cryofuge 5500i Cryofuge 5500i Multifuge 4KR Catalog Number 75004471 75004479 75004461 Weight of empty Rotor kg 18 4 18 4 18 4 Max Cycle Number 20000 20000 20000 Maximum permissible Lo...

Страница 9: ...mple Heating at nmax C referred to Ambient Temperature of 23 25 C Run Time 60 Minutes 1 1 1 Max Speed nmax rpm at 4 C 4400 4400 4400 Aerosol tight no no no Autoclavable no no no Table 1 4 200 V 50 Hz Round Bucket 75006477 Centrifuge Cryofuge 5500i Catalog Number 75004479 Weight of empty Rotor kg 18 4 Max Cycle Number 30000 Maximum permissible Load g 4 x 1400 Maximum Speed nmax rpm 4400 Maximum RCF...

Страница 10: ...s 4 Max Speed nmax rpm at 4 C 4400 Aerosol tight yes with cap 75006479 Permissible temperature range autoclavable C 121 tested by TÜV Product Service GmbH Hamburg Table 1 6 200 V 50 Hz Double Rectangular Bucket 75006436 Centrifuge Cryofuge 5500i Catalog Number 75004479 Weight of empty Rotor kg 17 9 Maximum Number of Cycles at Maximum Speed 12000 Maximum permissible Load g 4 x 1900 Maximum Speed nm...

Страница 11: ... 75006421 Permissible temperature range autoclavable C 121 tested by TÜV Product Service GmbH Hamburg Table 1 8 120 V 60 Hz Double Rectangular Bucket DoubleSpin 2 x 250 ml 75006478 Centrifuge Cryofuge 5500i Catalog Number 75004475 Weight of empty Rotor kg 18 4 Max Cycle Number 20000 Maximum permissible Load g 4 x 1500 Maximum Speed nmax rpm 4400 Maximum RCF Value at nmax 4654 Radius max min cm 21 ...

Страница 12: ... kg 17 9 Maximum Number of Cycles at Maximum Speed 12000 Maximum permissible Load g 4 x 1900 Maximum Speed nmax rpm 4400 Maximum RCF Value at nmax 5476 Radius max min cm 25 3 11 0 Acceleration Braking Time s 110 90 Sample Heating at nmax C referred to Ambient Temperature of 23 25 C Run Time 60 Minutes 1 Max Speed nmax rpm at 4 C 4400 Aerosol tight no Autoclavable no Technical Data ...

Страница 13: ...th cap 75006421 yes with cap 75006421 Permissible temperature range autoclavable C 121 121 Table 1 11 230 V 50 60 Hz Double Rectangular Bucket DoubleSpin 2 x 250 ml 75006478 Centrifuge Catalog Number 75004473 75004481 Weight of empty Rotor kg 18 4 18 4 Max Cycle Number 20000 20000 Maximum permissible Load g 4 x 1500 4 x 1500 Maximum Speed nmax rpm 4400 4400 Maximum RCF Value at nmax 4654 4654 Radi...

Страница 14: ...f 23 25 C Run Time 60 Minutes 1 1 Max Speed nmax rpm at 4 C 4400 4400 Aerosol tight no no Autoclavable no no Table 1 13 200 V 50 Hz Round Bucket 75006477 Centrifuge Catalog Number 75004481 Weight of empty Rotor kg 18 4 Max Cycle Number 30000 Maximum permissible Load g 4 x 1400 Maximum Speed nmax rpm 4400 Maximum RCF Value at nmax 5346 Radius max min cm 24 7 8 8 Acceleration Braking Time s 95 85 Sa...

Страница 15: ...eed nmax rpm at 4 C 4400 Aerosol tight yes with cap 75006479 Permissible temperature range autoclavable C 121 tested by TÜV Product Service GmbH Hamburg Table 1 15 200 V 50 Hz Double Rectangular Bucket 75006436 Centrifuge Catalog Number 75004481 Weight of empty Rotor kg 17 9 Maximum Number of Cycles at Maximum Speed 12000 Maximum permissible Load g 4 x 1900 Maximum Speed nmax rpm 4400 Maximum RCF ...

Страница 16: ...ermissible temperature range autoclavable C 121 tested by TÜV Product Service GmbH Hamburg Table 1 17 120 V 50 Hz Double Rectangular Bucket DoubleSpin 2 x 250 ml 75006478 Centrifuge Catalog Number 75004477 Weight of empty Rotor kg 18 4 Max Cycle Number 20000 Maximum permissible Load g 4 x 1500 Maximum Speed nmax rpm 4400 Maximum RCF Value at nmax 4654 Radius max min cm 21 5 11 5 Acceleration Braki...

Страница 17: ...ximum Number of Cycles at Maximum Speed 12000 Maximum permissible Load g 4 x 1400 Maximum Speed nmax rpm 4400 Maximum RCF Value at nmax 5346 Radius max min cm 24 7 8 8 Acceleration Braking Time s 85 85 Sample Heating at nmax C referred to Ambient Temperature of 23 25 C Run Time 60 Minutes 10 Max Speed nmax rpm at 4 C 4100 Aerosol tight no Autoclavable no Sorvall RC 4 Technical Data ...

Страница 18: ......

Страница 19: ...Thermo Scientific 75006476 LH 4000W 2 1 2 Accessories Contents Rotor data on page 2 2 Accessories on page 2 2 ...

Страница 20: ...IN 34 5 x 177 39 16 green 75008140 5 x 50 ml conical 29 5 x 177 35 5 20 light green 75006533 2 x 100 ml DIN 45 0 x 177 47 5 8 light blue 75008142 1 x 150 ml DIN 180 ml bottle 56 6 x 132 128 4 white 75006498 1 x 175 225 ml conical 250 ml bottle 62 x 177 75 4 nature 75008144 1 x 250 ml Bottle 59 x 190 4 nature 75006649 1 x 250 ml Corning tube conical 61 5 x 190 4 nature 75008147 1 x 500 ml Nalge tub...

Страница 21: ...ersal container 25 5 x 132 145 32 0 32 blue green 75006459 4 x 45 50 ml flat and round bottom 29 5 x 125 145 35 0 32 blue 75006491 3 x 50 ml DIN 34 5 x 127 148 38 0 24 green 75006460 4 x 50 ml conical 29 5 x 129 149 35 5 32 light green 75006461 1 x 100 ml DIN 45 0 x 138 66 0 8 light blue 75006462 1 x 150 ml DIN 180 ml bottle 56 6 x 147 66 0 8 grey blue 75006463 1 x 50 ml conical 250 ml bottle An a...

Страница 22: ......

Страница 23: ...Thermo Scientific 75006476 LH 4000W 3 1 3 Rotor Installation Contents Rotor Installation on page 3 2 Removing the Rotor on page 3 2 ...

Страница 24: ...or lid Removing the Rotor To remove the rotor proceed as follows 1 Open the centrifuge door 2 Thread the fastening tool in the centrifuge spindle counterclockwise Hold the rotor with the other hand into position 3 At the same time pull the rotor directly upwards with both hands and remove it from the centrifuge spindle Make sure not to tilt the rotor while doing this CAUTION Unapproved or incorrec...

Страница 25: ...Thermo Scientific 75006476 LH 4000W 4 1 4 Rotor Loading Contents Before a Run on page 4 2 Proper Loading on page 4 2 Improper Loading on page 4 3 Maximum Loading on page 4 3 Cycle Counter on page 4 4 ...

Страница 26: ...of the used materials using the chemical compatibility chart on page B 1 5 Make sure the tubes or bottles do not touch the bucket lid 6 Close all sample containers before placing them in the bucket 7 Check the rotor bolts and apply grease 75003786 before using for the first time 8 Check that each bucket or micro plate carrier can swing freely by moving it carefully with your hand Weigh the bucket ...

Страница 27: ...he safety system of the centrifuge requires that you do not overload the rotor There are two options available for centrifuging samples whose weight including adapter exceeds the maximum permissible load Reduce the fill level Reduce the speed Use the table or the formula Actual Load maximum speed 600 6000 620 5902 640 5809 660 5721 680 5636 700 5555 720 5477 740 5403 760 5331 780 5262 800 5196 820...

Страница 28: ...um number of cycles for buckets is marked on the buckets themselves Service life examples 880 4954 900 4899 Actual Load maximum speed n per n max maximum load actual load WARNING Replace the rotor when the specified number of cycles is reached Due to the mechanical load a rotor can break and thus damage the centrifuge Replace the buckets when the specified number of cycles is reached Usage profile...

Страница 29: ...ermo Scientific 75006476 LH 4000W 5 1 5 Aerosol tight Applications Contents Basic Principles on page 5 2 Closing aerosol tight on page 5 2 Fill Level on page 5 3 Checking the Aerosol Tightness on page 5 3 ...

Страница 30: ...e bucket aerosol tight by flapping both levers down until you can hear them locking into place Figure 5 1 Rectangular Bucket with Cap CAUTION Aerosol tight rotors and tubes may only be opened in an approved safety workbench when centrifuging dangerous samples Mind the maximum permissible load CAUTION Be sure to check all sealings before starting any aerosol tight applications CAUTION If the levers...

Страница 31: ...he buckets depends on the microbiological test process in accordance with the EN 61010 2 020 Appendix AA Whether or not a bucket is aerosol tight depends primarily on proper handling Check as needed to make sure your bucket is aerosol tight The careful inspection of the seals and seal surfaces for signs of wear and damage such as cracks scratches and embrittlement is extremely important Aerosol ti...

Страница 32: ...be inspected in order to assure that they are correctly seated and are not worn or damaged Lubricate all seals lightly Damaged seals must be replaced immediately When loading the rotor ensure that the rotor lid closes securely Damaged or clouded rotor caps must be replaced immediately Quick Test CAUTION This quick test is not suited for validating the aerosol tightness of your bucket Check the sea...

Страница 33: ...476 LH 4000W 6 1 6 Maintenance and Care Contents Cleaning Intervals on page 6 2 Cleaning on page 6 2 Disinfection on page 6 3 Decontamination on page 6 4 Autoclaving on page 6 5 Service of Thermo Fisher Scientific on page 6 5 ...

Страница 34: ...rature must never exceed 50 C since higher temperatures could damage the material and shorten the lifetime of the parts Use only disinfectants with a pH of 6 8 Dry aluminum parts off with a soft cloth Clean centrifuge and accessories as follows 1 Open the centrifuge 2 Turn off the centrifuge 3 Pull out the power supply plug Maintenance Recommended interval Clean rotor daily or when polluted Access...

Страница 35: ...e rotor should be treated preferably with a neutral disinfectant Contact the Service Department of Thermo Fisher Scientific for questions regarding the use of other disinfectants Disinfect the rotor and accessories as follows 1 Open the centrifuge 2 Turn off the centrifuge 3 Pull out the power supply plug CAUTION When cleaning do not allow liquids especially organic solvents to get on the drive sh...

Страница 36: ...e bolts with bolt grease 75003786 Decontamination Decontaminate rotor and centrifuge immediately whenever radioactive material has spilled during centrifugation For general radioactive decontamination use a solution of equal parts of 70 ethanol 10 SDS and water 1 Open the centrifuge 2 Turn off the centrifuge 3 Pull out the power supply plug 4 Remove the buckets from the rotor 5 Remove the centrifu...

Страница 37: ...lat surface Rotors and adapter can be autoclaved at 121 C The maximum permissible autoclave cycle is 20 minutes at 121 C Autoclaving of Polypropylene Bottle 75006613 1 Remove the seal from the cap and wrap it in aluminum foil before autoclaving 2 Screw the cap slightly onto the bottle before autoclaving 3 After autoclaving place the seal back in the cap in a sterile work bench Service of Thermo Fi...

Страница 38: ...otor the fixation of the rotor and the drive shaft Thermo Fisher Scientific offers inspection and service contracts for this work Any necessary repairs are performed for free during the warranty period and afterwards for a charge This is only valid if the centrifuge has only been maintained by a Thermo Fisher Scientific service technician ...

Страница 39: ...24 1000 8 8 24 7 98 276 1100 8 8 24 7 119 334 1200 8 8 24 7 142 398 1300 8 8 24 7 166 467 1400 8 8 24 7 193 541 1500 8 8 24 7 221 621 1600 8 8 24 7 252 707 1700 8 8 24 7 284 798 1800 8 8 24 7 319 895 1900 8 8 24 7 355 997 2000 8 8 24 7 394 1105 2100 8 8 24 7 434 1218 2200 8 8 24 7 476 1337 2300 8 8 24 7 520 1461 2400 8 8 24 7 567 1591 2500 8 8 24 7 615 1726 2600 8 8 24 7 665 1867 2700 8 8 24 7 717...

Страница 40: ... 4300 8 8 24 7 1819 5106 4400 8 8 24 7 1905 5346 Table A 2 Double Rectangular Bucket 75006478 rpm Rmin Rmax RCF Rmin RCF Rmax 300 11 5 21 5 12 22 400 11 5 21 5 21 38 500 11 5 21 5 32 60 600 11 5 21 5 46 87 700 11 5 21 5 63 118 800 11 5 21 5 82 154 900 11 5 21 5 104 195 1000 11 5 21 5 129 240 1100 11 5 21 5 156 291 1200 11 5 21 5 185 346 1300 11 5 21 5 217 406 1400 11 5 21 5 252 471 1500 11 5 21 5 ...

Страница 41: ... 5 21 5 1400 2618 3400 11 5 21 5 1486 2779 3500 11 5 21 5 1575 2945 3600 11 5 21 5 1666 3115 3700 11 5 21 5 1760 3291 3800 11 5 21 5 1857 3471 3900 11 5 21 5 1956 3656 4000 11 5 21 5 2057 3846 4100 11 5 21 5 2161 4041 4200 11 5 21 5 2268 4240 4300 11 5 21 5 2377 4444 4400 11 5 21 5 2489 4654 Table A 3 Double Rectangular Bucket 75006436 rpm Rmin Rmax RCF Rmin RCF Rmax 300 11 25 3 11 25 400 11 25 3 ...

Страница 42: ...2400 11 25 3 708 1629 2500 11 25 3 769 1768 2600 11 25 3 831 1912 2700 11 25 3 897 2062 2800 11 25 3 964 2218 2900 11 25 3 1034 2379 3000 11 25 3 1107 2546 3100 11 25 3 1182 2718 3200 11 25 3 1259 2896 3300 11 25 3 1339 3080 3400 11 25 3 1422 3270 3500 11 25 3 1507 3465 3600 11 25 3 1594 3666 3700 11 25 3 1684 3872 3800 11 25 3 1776 4084 3900 11 25 3 1871 4302 4000 11 25 3 1968 4526 4100 11 25 3 2...

Страница 43: ... S S Formic Acid 100 S M U U U S M U U S S U S U S U Ammonium Acetate S S U S S S S S S S S S S U S S S S S S S S S S Ammonium Carbonate M S U S S S S S S S S S S S U U S S S S S S M S S S Ammonium Hydroxide 10 U U S U S S M S S S S S S U M S S S S S S S S S M S Ammonium Hydroxide 28 U U S U S U M S S S S S U S U M S S S S S S S S S M S Ammonium Hydroxide conc U U U U S U M S S S U S U U S S S M S...

Страница 44: ... S S M M S U U S U M U U M M M U S S S S U U Ferric Chloride U U S M S M S S S S M U S S Acetic Acid Glacial S S U U S S U M S U S U U U U U M S U M U S U U S U Acetic Acid 5 S S M S S S M S S S S S M S S S S S S S M S S M S S M Acetic Acid 60 S S U U S S U S M S U U M U S M S M S M S M U S M U Ethyl Acetate M M U U S S M M S S U S U M U U S S U U S M M S U U Ethyl Alcohol 50 S S S S S S M S S S S...

Страница 45: ... S S M S S S S M S M U S S Kerosene S S S S S S U S M U S U M M S M M M S S U S S U S Sodium Chloride 10 S S S S S S S S S S S S S S S S S S M S Sodium Chloride sat d U S U S S S S S S S S S S S S S M S Carbon Tetrachloride U U M S S U M U S U U S U M U S S M M S M M M M U S S Aqua Regia U U U U U U U U U U U S M Solution 555 20 S S S S S S S S S S S S S S S S S S S S Magnesium Chloride M S S S S ...

Страница 46: ...loric Acid 10 U U S U U S M M M U M S M M M S U S S Perchloric Acid 70 U U U U U S U M U U M U U U M M U M S U U S U S Phenol 5 U S U S M M S U M U U S U M S M S U U S U M M M S Phenol 50 U S U S U M S U M U U U U U S U M U U S U U U M S Phosphoric Acid 10 U U M S S S U S S S S U S S S S S S S S S U M U S S Phosphoric Acid conc U U M M U S M S U U M M S S S M S M S U M U S Physiologic Media Serum ...

Страница 47: ... X 100 S S S S S S S S S S S S S S S S S S S S S S S S S Urea S U S S S S S S S M S S S S S S S M S S Hydrogen Peroxide 10 U U M S S U U S S S U S S S M U S S S S S S M S U S Hydrogen Peroxide 3 S M S S S S S S S S S S S S M S S S S S S S S S S Xylene S S U S S S M U S U U U U U U M U M U U U S U M S U S Zinc Chloride U U S S S S U S S S S S S S S S S S S S S S S U S S S Zinc Sulfate U S S S S S S...

Страница 48: ...476 LH 4000W Thermo Scientific Chemical resistance data is included only as a guide to product use No organized chemical resistance data exists for materials under the stress of centrifugation When in doubt we recommend pretesting sample lots ...

Страница 49: ...s terms and pricing are subject to change Not all products are available in all countries Please consult your local sales representative for details Shown pictures within the manual are examples and may differ considering the set parameters and language Pictures of the user interface within the manual are showing the English version as example Australia 61 39757 4300 Austria 43 1 801 40 0 Belgium ...

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