Thermo Scientific 074114, Руководство оператора

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3 • Operation and Maintenance
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3.1.4 Turn On the Lamps
To turn on the lamps, click UV Lamp and Vis Lamp on the Chromeleon
7 ePanel (see Figure 3-1 ) or the Chromeleon 6.8 Control panel (see
Figure 3-2 ).
When a detector lamp is turned on for the first time after the detector
power is turned on, wavelength calibration is performed automatically.
Calibration starts shortly after the lamp is ignited.
3.2 System Equilibration
Before you can begin using the Dionex PDA for sample analysis, the
chromatography system must be equilibrated. To equilibrate the system, perform
the following tasks:
•
Pump the starting solvent through the entire system until the system is free of
any other liquid composition.
•
Heat or cool all temperature-controlled devices (for example, a column
compartment) to the temperature required for the application.
•
Set the detector wavelength(s) and turn on the lamp(s).
•
Calibrate the wavelength(s).
•
Monitor the pump pressure and verify that the reading is correct for the
application and is stable.
•
Monitor the detector signal and verify that the baseline signal is at the
expected reading for your application and is stable. This may not be the case if
the solvent composition has been modified, or if the light path contains air
bubbles. See
Section 4.5 and Section 4.6 for troubleshooting information.
NOTE To achieve optimum results, allow 20 to 30 minutes for
the lamp(s) to stabilize before beginning operation. For
sensitive applications work or for detector validation,
allow at least 2 hours for the lamps to stabilize.
Select one of the following methods to perform the equilibration tasks:
•
Select operating commands and parameters directly from the Chromeleon 7
ePanel Set or the Chromeleon 6.8 Control panel. See
Section 3.3.1 for details
about direct control.