Teledyne UA-6, Инструкция по установке и эксплуатации

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UA-6 Detector
Section 3 Operation
3-12
tings will help eliminate false peak cutting due to baseline and
bubble noise.
3.10 Built-in Chart
Recorder
The UA-6 has a built-in recorder that provides a permanent
record of chromatograms that can be conveniently taped in a lab
notebook. This paper is printed with a 10 cm wide grid strip with
2 mm minor and 1 cm major divisions. Increasing absorbance
causes the pen to deflect to the left.
The internal recorder in the UA-6 may be used as a stand-alone
recorder (see Section 3.15).
3.11 Built-In Peak
Separator
UA-6 detectors include a built-in peak separator for collecting
chromatography peaks with a fraction collector. The peak sepa-
rator is a change of slope detector which cuts peaks based on the
first derivative of the curve and not an absorbance threshold or
level. The peak separator is designed to collect all peaks,
regardless of their degree of resolution.
3.11.1 PEAK SEPARATOR
Switch
Peak cutting with the peak separator is largely automatic. The
PEAK SEPARATOR switch is used to fine tune peak cutting
based on the expected peak widths at baseline. The peak sepa-
rator can most accurately respond to baseline peak widths that
range from 0.2 to 2 times the selected peak duration. For peaks
shorter than 0.2X, the selected setting of the switch, the peak
separator may respond slightly slower in cutting the leading
edge of the peak. For peaks greater than 2X, the selected setting
of the switch the ending cut may be slightly early, resulting in a
certain loss of peak material. Ideally, the PEAK SEPARATOR
switch should be set such that the peak width of all important
peaks in the chromatogram are greater than 0.2, yet less than 2
times the setting of the switch.
Figure 3-8 Sample Chromatogram