2. General
2021-07
Instructions for Use for SUNRISE No. 30086638 Rev. No. 2.9
19
2.3.5
SUNRISE Instrument Configuration with Option
Temperature Control
Peltier-based temperature control.
PARAMETERS
CHARACTERISTICS
Temperature range:
Room temperature up to 42°C increments of 0.1°C
Accuracy
t/- 0.2°C (max. +/- 0.5°C)
Preheating time
30 min
The SUNRISE specification of the temperature control unit (typical ac/-
0.2°C) applies if everything (reader, microplate, reagents, samples) has already
target temperature.
There is no specification for the heating up period and we are aware that within
this period not all wells are heated up equally. This effect will be seen especially
when reagents are not preheated.
During preheating the plate transport with a 96 well microplate loaded has to be
inside of the instrument. Only in this way a homogenous temperature is received
in the instrument. The microplate must not be the same as the one used for
measurement but should be clean to keep influences on the following
measurement low.
Every time the plate transport is moved it will take up to 1 min to reach again a
homogenous temperature inside of the instrument.
Bear in mind that if non-preheated microplate and/or reagents are added,
temperature control will take a certain time depending on the temperature
difference between microplates.
We recommend a longer measurement interval (e.g. 1 min) to increase
homogenous temperature distribution. Keep the difference between target
temperature and the temperature of microplate with reagents and samples lower
than 20 °C (68 °F).
