7. Quality Control
98
Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
2011-09
7.4 Test
Instructions
7.4.1 Fluorescence
Intensity
7.4.1.1 Detection Limit Top Fluorescein 384-well Plate
Pipette 100 µl of the 1 nM Fluorescein solution and 100 µl of the blank solution
(0.01 M NaOH) into the wells of a Greiner 384-well plate (black, flat bottom)
according to the plate layout.
Material:
Greiner 384-well plate, flat bottom, black
100 µl pipette (8-channel) + tips
1 nM Fluorescein (diluted in 0.01 M NaOH)
0.01 M NaOH
Plate Layout:
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1
2
3
4
5
6
7
8
9
10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
A
F B F B F B
F
B
F
B
F B
B
C
F B F B F B
F
B
F
B
F B
D
E
F B F B F B
F
B
F
B
F B
F
G
F B F B F B
F
B
F
B
F B
H
I
F B F B F B
F
B
F
B
F B
J
K
F B F B F B
F
B
F
B
F B
L
M
F B F B F B
F
B
F
B
F B
N
O
F B F B F B
F
B
F
B
F B
P
(F….100 µl 1 nM Fluorescein; B…..100 µl 0.01 M NaOH = Blank)
