4. Optical System
46
Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0
2011-09
0
10
20
30
40
50
60
70
80
90
100
460
490
520
550
580
610
640
670
700
Wavelength [nm]
T
ran
sm
issi
o
n
[
%
]
Figure 25: Transmission spectrum of filter ‘Lumi Orange‘.
0
10
20
30
40
50
60
70
80
90
100
400
430
460
490
520
550
580
Wavelength [nm]
T
ransm
is
si
on
[
%
]
Figure 26: Transmission spectrum of filter ‘Lumi Blue‘.
Photon Counting Module (PCM)
The PCM, containing a channel photomultiplier, is designed for applications in
chemo- and bioluminescence. The channel photomultiplier provides a high
dynamic range enabling luminescence measurement with strong variations in
light levels. The exceptionally low noise and high sensitivity allow detection at
very low light levels.
4.4.2 Luminescence
Detection
STOP
Caution
Switch on the instrument at least 15 minutes before starting a
luminescence measurement to ensure stable conditions for the
measurement.
The
INFINITE M1000 PRO
luminescence detection system utilizes the single
photon counting measurement technique. This is based on a dedicated
luminescence PMT with appropriate measurement circuitry. This technique is
very robust against noise. It is preferred for measurements at very low light
levels.
For best performance, it is recommended to use white plates for luminescence
measurements.
Note
The results of luminescence measurements are always displayed in
counts per second, regardless of the integration time used.
