tecan INFINITE M1000 PRO, Инструкция по эксплуатации

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7. Quality Control
106 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 2011-09
7.4.2 Time Resolved Fluorescence
7.4.2.1 Detection Limit Europium 384-well Plate:
Pipette 100 µl of the reagents into the wells of a Greiner 384-well plate (white, flat
bottom) according to the Plate Layout.
Material:
Greiner 384-well plate, flat bottom, white
200 µl pipette (8-channel) + tips
1 nM Europium Standard (Wallac)
Enhancement Solution
Plate Layout:
<> 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
A Eu B B B B B B B B B B B B
B
C Eu B B B B B B B B B B B B
D
E Eu B B B B B B B B B B B B
F
G B B B B B B B B B B B B
H
I B B B B B B B B B B B B
J
K B B B B B B B B B B B B
L
M B B B B B B B B B B B B
N
O B B B B B B B B B B B B
P
(Eu….100 µl 1 nM Europium; B…..100 µl Enhancement Solution = Blank)
Measurement Parameters:
Measurement mode: Fluorescence Top
Excitation wavelength: 345 nm
Emission wavelength: 617 nm
Excitation bandwidth:
20 nm
Emission bandwidth: 20 nm
Gain: Optimal
Read Mode: Mode 2 (100 Hz)
Number of flashes: 12
Lag time: 100 µs
Integration time: 400 µs
Plate definition file: GRE384fw.pdfx, part of the plate: A1 – O13
Z-Position: Calculate from A1
Evaluation:
Calculate the detection limit in pM:
B
B Eu
stdev * 3 *
) mean (mean
1000
imit DetectionL
−
=
mean
Eu
average of wells filled with 1 nM Europium
mean
B
average of wells filled with Blank
stdev
B
standard deviation of wells filled with Blank