2. General
2.5 Washing Sequence Modes
The instrument can wash a microplate using the following sequence modes:
Plate Mode
Each process step is performed on all of the strips sequentially,
before proceeding to the next process step. The entire plate is
processed for the defined soaking time (as opposed to the Strip
Mode).
Strip Mode
The entire Wash program is performed on a strip before
proceeding to the next strip. Each individual strip is processed for
the defined soaking time, before proceeding to the next strip.
2.5.1 Washing
Modes
The instrument can use the following washing modes to wash a microplate:
Overflow Washing
For ELISA assays and Cellular assays
.
Overflow
washing consists of a simultaneous aspiration and
dispense step. It creates a circular flow of wash
buffer in the well.
Bottom Washing
For ELISA assays
.
Dilution Washing
Dilution washing consists of a sequence of
aspiration and dispense steps.
Fig. 2-5 Washing Positions
Overflow Washing
Bottom Washing
Aspiration step
Dilution Washing
Dispense step
2-10
Instructions for Use for Columbus Washer No. I 109 006 Rev No. 2.2
May 2003
