aCOLyte 3 HD
2 Sector Spiral Batch set-up - Quick Guide
Start aCOLyte 3 HD software
Log onto aCOLyte 3 HD
Capture image
Select the image tab and check the
live box
Create batch
Click New Batch
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Position plate
Insert the plate onto the plate holder.
Press lighting button on the front of
the aCOLyte 3 HD to obtain an image
which gives the best contrast
between
the
colonies
and
background being used
Adjust the exposure by increasing/
decreasing the sliding scale
Capture image
Click
Click
Slide
Click
Plate configuration
Select Plate Configuration to set
plate size, sample volume and
threshold. Defaults are Circular
plate 90mm, Spiral volume
100µl and threshold of 20.
Change if necessary
Select
Select
Choose an application
Select Spiral Plate
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