Sutter Instrument P-97, Инструкции по эксплуатации

Страница: 28 / 66

18
Recommendations
Table 3-3. Maximum heat and recommended starting values for different filament shapes.
Filament Maximum/Recommended Value(s)
Maximum Program HEAT value(s) Ramp value + 30 units.
Box
Recommended starting value Ramp value.
Maximum program HEAT value(s) Ramp value + 35 units.
Trough
Recommended starting value Ramp value+ 15 units.
Additional guidelines for setting program HEAT values relative to RAMP TEST values are
given in the Programming Section of this manual. HEAT settings over RAMP +35 can
damage or burn out the filament.
3.3.2 CHANGE AIR PRESSURE < 2 >
This control sets the pressure generated by the air compressor during the active cooling
phase of the pull cycle. Each time a pull is started the air pressure is corrected before the
filament turns on. A value must be entered each time this function is accessed. Changes of
less than 10 units will not be noticeable. Air pressure is reported on the program header line.
Range 1 to 999
Default 500
Recommendations
For thick walled glass 500
For thin walled glass 200 to 300
3.3.3 AIR MODE < 3 >
Select between one of two modes of cooling during the active cooling phase of the pull cycle.
The mode is reported in the fourth field of each program line.
TIME MODE Controls the length of time the gas is on during the cooling
phase. One unit of TIME is equal to 1/2 millisecond (e.g., at a
setting of 150 the cooling air will be on for 75ms).
DELAY MODE Controls the onset of the hard pull. The air is turned on for
300 ms during the cooling phase. One unit of DELAY is
equal to one millisecond (e.g., a setting of 100 will delay the
onset of the hard pull by 100 ms).
Default TIME MODE
Recommendations
Use the TIME MODE for: •
Sharp microelectrodes and patch pipette programs using
P-97 FLAMING/BROWN MICROPIPETTE PULLER OPERATION MANUAL – REV. 2.43 - DOM (20161118)