Soft Imaging System ColorView III, Руководство пользователя

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ColorView III • Camera Control
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How to... This is how you set the sharpness monitor for good acquisitions:
1)
Set all other parameters (microscope settings, exposure time, white
balance) optimally. The application of the sharpness monitor should be
the last step before image acquisition.
2)
Acquire a snapshot.
3)
Use the Camera Control command.
4)
Click the Set ROI for sharpness monitor button to interactively define a
rectangular area of the image for the sharpness monitor.
"
To do this, select an area of the specimen for which a good sharp-
ness setting is especially important.
5)
Begin a live acquisition.
"
The Set ROI for sharpness monitor button becomes inactive.
6)
Click the sharpness monitor on/off button to open the sharpness mon-
itor dialog box.
"
The sharpness monitor begins with a value of 50%.
7)
Vary the focus settings and observe the sharpness monitor while doing
so.
"
The sharpness monitor bar shows the current sharpness, the green
markation shows the highest achieved sharpness.
"
Mind the exposure time when setting the focus. The sharpness
monitor alters itself with a certain amount of delay when using larg-
er exposure times (> 100ms). Vary the focus settings in small inter-
vals and wait each time until the sharpness monitor has been
adapted as well.
8)
Find the focus settings by which the sharpness monitor is at its maxi-
mum and use these settings for the acquisition.
Partial Readout
The partial readout is a method for fast live acquisition. A rectangular part of
the image is defined for the partial readout to which the readout is limited
during the live acquisition. A higher frame rate is attained due to the smaller
amount of data to be transferred.
Available The live overlay must be enabled for the partial readout. The live overlay can
be enabled via the Display tab located in the Configure Input dialog box.
Partial Readout and Clip-
ping
The partial readout and clipping functions both lead to smaller images than
those possible with the camera. The partial readout already works when the
image is read out of the camera; the image areas which are faded out by this
function are not transferred to the computer. The clipping, however, is then
calculated out of the complete image data in the computer; an acceleration
in the image acquisition is not intended. The frame rate, due to the very high
pixel count of the camera, using full resolution is often not enough to enable
good focussing during live-mode. In order to attain both high resolution and
high frame rates, you must do the following:
1)
Start the live acquisition using full resolution.
2)
Set the ROI for Partial Readout onto an important image detail.
3)
Activate the Partial Readout .
4)
Do the required settings.
5)
Deactivate the Partial Readout .
Related Topics
The Display tab 39