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ColorView III • Camera Control
20
Histogram cal-
culation on ROI
Click the
Histogram calculation on full image button
to carry out the contrast
optimization on the basis of a rectangular area within the image. You can
use this option if relevant details are visible in a part of the image. The dis-
play can thus be optimized for this area.
Set ROI for his-
togram
Click the
Set ROI for histogram
button to interactively set the rectangular
area of the image for optimizing the contrast. Pull the ROI's frame shown in
the image document to the desired position and set its size by moving the
mouse and keeping the right mouse button depressed. Rightclick to confirm
position and size of the ROI.
Use fixed scal-
ing
Click the
Use fixed scaling
button to use a fixed scaling amplification. In con-
trast to the automatic gain display, the fixed scaling utilizes a predefined sig-
nal range which is manipulated so that it uses the entire dynamic area.
When using the fixed scaling, the image is no longer displayed optimally if
the illumination or sample are altered.
Automatically
adjust fixed
scaling
Click the
Automatically adjust fixed scaling
button to automatically set the
scaling on the current conditions. This button is only available during an ac-
tive live acquisition.
Manually ad-
just fixed scal-
ing
Click the
Manually adjust fixed scaling
button to manually set the upper right
hand limit for the fixed scaling. The
Fixed scaling
dialog box is opened.
Move the
Right limit
slide control or directly enter the desired numerical val-
ue into the text box.
Black balance
The black balance is used for background correction. The program calcu-
lates the mean value for each color channel in an area (ROI) which you de-
fined. These values are subtracted from all pixels in the entire image. The
black balance is to be used predominately for fluorescence acquisitions in
order to balance the undesired background in the acquisition. A discolora-
tion of the images occurs if the black balance is used with brightfield acqui-
sitions.
Functionality
The black balance is carried out based on the rectangular area in the image,
in which a uniformly dark-gray to black signal is to be located. The mean sig-
nal value is determined for each color channel within this area. This value is
reduced from each pixel when applying the black balance.
How to...
This is how you carry out a black balance:
1)
Take a snapshot which contains a suitable area to be used for black
balance.
2)
Use the
Camera Control
command.
3)
Click the
Set ROI for Black Balance
button to adduct an area of the im-
age for the black balance.
4)
Pull the ROI's red frame shown in the image document to a position
which contains an area which is as uniformly dark as possible.
Содержание ColorView III
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