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Operator’s Manual

For Automated Western Blot Processing

Содержание Blot Washer

Страница 1: ...Operator s Manual For Automated Western Blot Processing...

Страница 2: ...cted by copyright All rights are reserved No part of this document may be photocopied reproduced or translated to another language without prior written consent of Stovall Life Science Inc Stovall Lif...

Страница 3: ...bing and Containers 12 Discarding Waste and Replacing Wash Buffer 13 Cleaning the Exterior Surface 13 Chapter 2 Creating and Running Programs Keys You Should Be Familiar With 15 Powering On the Blot W...

Страница 4: ......

Страница 5: ...roperly grounded The power cords have a three prong grounded plug that mates with a standard three prong grounded wall receptacle to minimize the possibility of electric shock The user should have the...

Страница 6: ...meplate located on the back panel Fuse Replacement Warning Before replacing a blown fuse turn all switches to the OFF position and unplug the instrument Figure 1 shows the location of the fuse in a sm...

Страница 7: ...An internal pump supplies positive pressure for liquid delivery and house vacuum aspirates waste liquids from blot containers Air pressure from the internal pump is directed through the blue pressure...

Страница 8: ...r front panel CHAPTER 1 System Familiarization and Assembly Software wash programs operate three solenoid valves to control the ow of liquids The normal condition of all valves is closed where by the...

Страница 9: ...ity by allowing a second delivery container to be connected Figure 5 Similar to the Wash bottle this second container is pressurized by the pump and liquid is dispensed to the blot container when Disp...

Страница 10: ...tubing are initially generous enough to accom modate different con gurations Once an optimal con guration has been determined for your lab you may want to shorten excessively long tubes for a more or...

Страница 11: ...ssure Tubing Blue Luer Lock Wash Delivery Tubing Green Luer Lock Vacuum Tubing Black Luer Lock Waste Tubing Orange Luer Lock Western Blot Incubation Box or Similar Blot Container Wash Bottle Waste Bot...

Страница 12: ...is completed by connecting one end of the single dispensing harness waste tube to the right side connector on the manifold and the other end to the aspiration bit attached to the blot container Make s...

Страница 13: ...racting it from a valve manually open the valve by pressing the circular button above the valve as shown in Figure 4 Stretch the tubing enough to slip it into the valve slot Manual open close button f...

Страница 14: ...con guration that delivers two different wash buffers A typical application for a second container is to deliver secondary antibodies Notice that the air pressure provided by the Blot Washer s pump is...

Страница 15: ...11 Figure 5 Tubing configuration for using the 140 ml syringe or other secondary delivery container waste tubing not shown 140 ml syringe or second delivery container...

Страница 16: ...should be clean for every experiment For best performance clean the delivery and waste tubing with water immediately after each use Disconnect the wash tubing from the green luer lock on top of the w...

Страница 17: ...the tubing to the top of the bottle Take the top off Replace the buf fer Occasionally you should wash out the buffer bottle after many repeated uses or if a different buffer is to be used Replace the...

Страница 18: ......

Страница 19: ...ur own programs you may want to develop a system to document each program for easy recall Keys You Should Be Familiar With Most of the keystrokes in creating a program are used to toggle be tween avai...

Страница 20: ...number of blots Three actions are possible when this display is shown a Press or to change the program number to any of the 20 programs b Press to run the speci ed program see Starting a Program belo...

Страница 21: ...iner s does not need to be programmed Aspiration occurs automatically according to the amount of liquid s originally dispensed to the blot containers Should the aspirating be too long or too short cal...

Страница 22: ...TEP 1 TYPE DELAY The top line of the display indicates this is step 1 of program number 1 The and keys change the step type between DELAY WASH and INCUBATE 5 Press to accept DELAY as the step type EDI...

Страница 23: ...re 2 Chapter 1 Typically Dispense Valve 1 is used unless you want to dispense from a secondary delivery container 9 Press to accept WASH VALVE 1 EDIT P1 STEP 2 DISPENSE 50 ML The and keys increment th...

Страница 24: ...es are needed The third step will be used to dispense secondary antibodies to the blot container for incubation 13 Press to accept the step type EDIT P1 STEP 3 TYPE INCUBATE Typically secondary antibo...

Страница 25: ...This second and nal wash step uses the same prompts for wash parameters cycles etc as the wash in program step two described earlier 7 10 above Wash parameters would normally be entered to match your...

Страница 26: ...tialdisplayisusedto select the number of blots This step is optional but recom mended especially after changing tubing or lling liquid bottles SYSTEM PRESSURE 23 KPA 2 Press to check the system pressu...

Страница 27: ...ation is paused Quickly press to again while the Press Stop message is displayed If you wait too long and the message changes pressing will just start the routine again Using the Quad Harness If you p...

Страница 28: ...on the Blot Washer front panel 2 Turn on the Blot Washer and wait until prompted for the number of blots CHAPTER 2 Creating Wash Sequences 3 Press four times until 4 OF QUAD is displayed indicating t...

Страница 29: ...Incu bation Boxes on a shaker The Belly Dancer Stovall Life Science Inc Greensboro NC 4 Press to set the number of blots and continue SELECT PROGRAM NUMBER 1 5 Press once to increment the program numb...

Страница 30: ...p in the program should be set to dispense 40 ml Blot Washer already knows that 40 ml should be delivered to each container if 2 OF QUAD 3 OF QUAD or 4 OF QUAD is chosen for NUMBER OF BLOTS Delivery r...

Страница 31: ...NCUBATE and WASH pro grams 11 13 3 step WASH INCUBATE and WASH programs 14 16 2 step WASH WASH programs 17 19 1 step WASH programs 20 A 4 step test program used during factory testing that can be modi...

Страница 32: ......

Страница 33: ...ength of the house vacuum For best results place the delivery container above the blot container s Calibration may be needed if the dispense volume is inaccurate or aspiration is incomplete or goes on...

Страница 34: ...check the tightness of liquid container tops and tubing connections Calibrating Dispense Volume If the system pressure is correct as described above but the volume dispensed is still inaccurate the di...

Страница 35: ...rocessing Setting the Aspiration Vacuum Factor 1 Press once if TO CALIBRATE DISPENSE 100 ML is displayed or press twice if SELECT NUMBER OF BLOTS is displayed Manual open close button for Dispense Val...

Страница 36: ......

Страница 37: ...Western Incubation Boxes are available in various pack sizes See www slscience com Speci cations Console Dimensions 23 W x 9 6 D x 5 4 cm H 9 W x 5 9 D x 5 4 H Electrical 115V model BLWAA115S 50 60 h...

Страница 38: ...34 CHAPTER 4 Appendix Program Storage Capacity 20 programs with up to 4 steps per program Liquid Flow Control Valves 2 for delivery 1 for aspiration...

Страница 39: ...andards EN50081 1 1997 Generic emission standards for residential commercial and light industry environments EN50082 1 1997 Generic immunity standards for residential commercial and light industry env...

Страница 40: ...Printed Franklin s 30 Units 4 2 10...

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