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Sigma 147220, Руководство по эксплуатации

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Refrigerated Centrifuge with Heating System Sigma 3-18KHS 

  

5 Set-up and connection 

 

 

 

Version 08/2012, Rev. 1.21 of 08/05/2020 

• sb 

27 / 89 

Translation of the original operating manual, part no. 0701202 

 

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→

 

5.2.2 

 Customer-provided fuses  

Typically, the centrifuge must be protected with 16 Amp L or B fuses that 
are to be provided by the customer. 

 

 

NOTE 

To ensure safe disconnection in the event of a fault, an AC/DC-sensitive 
RCD (residual current device) must be integrated in the wiring system of 
the building. 

 

 

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Содержание 147220

Страница 1: ...e original operating manual sb 08 2012 Rev 1 21 of 08 05 2020 Sigma 3 18KHS part no 0701202 Sigma 3 18KHS from serial no 147220 Refrigerated Centrifuge with Heating System Operating Manual Please reta...

Страница 2: ...Refrigerated Centrifuge with Heating System Sigma 3 18KHS 2 89 Version 08 2012 Rev 1 21 of 08 05 2020 sb Translation of the original operating manual part no 0701202...

Страница 3: ...of 08 05 2020 sb 3 89 Translation of the original operating manual part no 0701202 Copyright by Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode am Harz Germany Tel 49 0 5522 5007 0 F...

Страница 4: ...Refrigerated Centrifuge with Heating System Sigma 3 18KHS 4 89 Version 08 2012 Rev 1 21 of 08 05 2020 sb Translation of the original operating manual part no 0701202...

Страница 5: ...l force 14 2 2 2 2 Density 14 3 Safety 15 3 1 Marking of the unit 15 3 2 Explanation of the symbols and notes 16 3 3 Responsibility of the operator 17 3 4 Operating personnel 17 3 5 Informal safety in...

Страница 6: ...tallation of a standard angle rotor with a hermetic lid 29 6 2 2 3 Installing a rotor with the g lock system 30 6 2 2 4 Installation of accessories 31 6 2 2 5 Adapters 32 6 2 2 6 Vessels 32 6 3 Contro...

Страница 7: ...8 1 3 Rotors buckets and carriers 62 8 1 4 Load bearing bolts 63 8 1 5 Glass breakage 64 8 2 Sterilisation and disinfection of the rotor chamber and accessories 64 8 2 1 Autoclaving 65 8 3 Service 66...

Страница 8: ...Refrigerated Centrifuge with Heating System Sigma 3 18KHS Table of contents 8 89 Version 08 2012 Rev 1 21 of 08 05 2020 sb Translation of the original operating manual part no 0701202...

Страница 9: ...a Standardm odule 010 Allgem eine Inform ationen 010 0020 Bestimm ungsgem e Verw endung Sigma 26 m od_1405318951312_68 docx 191709 2 1 1 2 Intended use Centrifuges are power driven machines that separ...

Страница 10: ...r dmodule 010 Allgemei ne Informati onen 010 0050 N ormen und Vorschriften 26 mod_1405318958202_68 docx 191765 2 1 1 5 Standards and regulations EC declaration of conformity see chapter 11 6 EC declar...

Страница 11: ...e 020 Aufbau und Wirkungsw eise 020 0010 Aufbau der Zentrifuge 26 m od_1405318982945_68 docx 191793 2 1 2 1 Layout of the centrifuge Pos 17 100 Sigma 110 BA Zentrifugen Sigma Pr ojekte 3 18KS 3 18KH S...

Страница 12: ...0020 Typenschild 3 18KHS 29 mod_1405321771072_68 docx 197617 3 1 2 1 2 Name plate 1 Manufacturer 2 Power consumption 3 Max speed 4 Max kinetic energy 5 Serial number 6 Part number 7 Type 8 Nominal vol...

Страница 13: ...cally as a function of the angular velocity The bigger the radius in the rotor chamber is and the higher the speed is the higher the centripetal acceleration is However the forces acting on the rotor...

Страница 14: ...rifugal force will be recalculated automatically by the control unit If the RCF is changed the speed will be adapted while the specified radius is maintained The speed gravitational field diagram prov...

Страница 15: ...1378 2 1 3 1 Marking of the unit The following symbols are used for all types of centrifuges manufactured by Sigma Dangerous voltage I On Power Hot surface 0 Off Power Caution Risk of bruising Name pl...

Страница 16: ...es and symbols to indicate hazards are used DANGER This symbol stands for a direct hazard to the life and health of persons Non observance of these symbols causes serious health problems up to life en...

Страница 17: ...y The centrifuge has to be maintained regularly see chapter 8 Maintenance and service Components that are not in a perfect state must be replaced immediately Pos 37 010 U niversalmodule Leerzeile 0 m...

Страница 18: ...g in compliance with the relevant standards Pos 44 010 U niversalmodule Leerzeile 0 m od_1202116244500_0 docx 114 1 Pos 45 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 030 Sicher heit 030 0060 0...

Страница 19: ..._0 docx 114 1 Pos 49 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 030 Sicher heit 030 0060 0040 C hemische und bi ologische Sicher hei t 26 m od_1405319028141_68 docx 192101 3 1 3 6 4 Chemical a...

Страница 20: ...ursting vessels can cause dangerous imbalances at high speeds Ensure that rotor and buckets are correctly fitted see chapter 6 2 2 1 Installation of a standard rotor Observe the instructions on the in...

Страница 21: ...ion cracks material abrasion etc After 10 years they must be inspected by the manufacturer After 50 000 cycles the rotor must be scrapped for reasons of safety If other data concerning the service lif...

Страница 22: ...a dialog box see chapter 7 2 Table of error codes Pos 64 010 U niversalmodule Leerzeile 0 m od_1202116244500_0 docx 114 1 Pos 65 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 030 Sicher heit 030...

Страница 23: ...gen Sigma Standar dmodule 030 Sicher heit 030 0100 R estrisiken 27 mod_1405319052885_68 docx 192339 2 1 3 9 Remaining hazards The centrifuge was built in accordance with the state of the art and in co...

Страница 24: ...m od_1405319161442_68 docx 192367 2 1 4 2 Storage conditions The centrifuge can be stored in its original packaging for up to a year Store the centrifuge only in dry rooms The permissible storage temp...

Страница 25: ...jekte 3 18KS 3 18KH S 040 Lagerung und Tr ansport 040 0050 Transportsicherung 3 18KS_KHS berschrift 29 mod_1405321789957_68 docx 197701 2 1 4 5 Transport safety device Pos 88 100 Sigma 110 BA Zentrifu...

Страница 26: ...the centrifuge It is important to allow sufficient time for drying min 24 h before the centrifuge can be used again Pos 93 010 U niversalmodule Leerzeile 0 m od_1202116244500_0 docx 114 1 Pos 94 100...

Страница 27: ...uss 050 0020 0020 Sicherungen bauseits au er 3 30KS 7 mod_1306312302397_68 docx 43513 3 1 5 2 2 Customer provided fuses Typically the centrifuge must be protected with 16 Amp L or B fuses that are to...

Страница 28: ...ndardm odul e 060 Betrieb_Spi ncontr ol L S 060 0020 Ei nschalten au er 6 16HS 27 mod_1405319201340_68 docx 192451 2 1 6 2 Switching the centrifuge on Press the mains power switch The display then ill...

Страница 29: ...rotor must be lifted and fastened again This ensures a proper connection between the rotor and the motor shaft CAUTION When using rotors for microtiter plate formats Ensure that the plate holders are...

Страница 30: ...urning it counter clockwise and remove the lid 1 Rotor tie down screw 2 Lid 3 Rotor Fig 5 Angle rotor with a hermetic lid Pos 111 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 11...

Страница 31: ...ts Always load opposite compartments of the rotors with the same accessories and fill to avoid imbalance Centrifugation with vessels of various sizes Working with vessel of various sizes is possible I...

Страница 32: ...venly Loading the edges of a bucket only is not permissible Pos 117 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 118 100 Sigma 100 BA Zentrifugen Sigma Standardm odul e 060 Betr...

Страница 33: ...ree buttons with integrated light emitting diodes and one function knob The display is divided into several different fields The various functions of the system can be called up by pressing and turnin...

Страница 34: ...be triggered during a normal deceleration e g in order to speed up the deceleration When a quick stop is triggered Quick stop will be displayed in the speed field NOTE A quick stop can be performed e...

Страница 35: ...he actual speed is displayed below this value The values are stated in revolutions per minute min 1 rpm and they depend on the RCF values see chapter 2 2 2 1 Speed radius and relative centrifugal forc...

Страница 36: ...peed is reached Select the field Runtime and press the function knob The display flashes when it is activated Turn the function knob from the time 0 00 10 anti clockwise or from the time 99 59 59 cloc...

Страница 37: ...unction knob in order to accept the data Fig 12 Rotor selection list here for a swing out rotor Automatic rotor identification The centrifuge automatically identifies the rotor that is currently being...

Страница 38: ...Standard menu shows the program that is currently loaded When the field is selected the program list is displayed for information on how to work with the programs please see chapter 6 3 3 Program mode...

Страница 39: ...No message will be issued when the set temperature is reached A parameter is changed except for the temperature or another input is made In this case the RAPID_TEMP program will be aborted No message...

Страница 40: ...runtime of the running centrifugation run For this purpose a green progress bar and percentage value are displayed in the program field Fig 15 Progress indicator during a centrifugation run After the...

Страница 41: ...me and temperature exception the RAPID_TEMP program will not be listed The order of the processes corresponds to their storage locations on the program list Empty storage locations will not be display...

Страница 42: ...still Depending on the substances that are to be centrifuged it may make sense to bring the centrifuge to a certain temperature prior to starting a centrifugation The temperature adaptation prevents t...

Страница 43: ...ward facing arrow in the speed field Values between 1 2 and 10 0 g cm3 are possible Centrifugation monitoring The centrifugation monitoring function enables the continuous monitoring of the speed and...

Страница 44: ...ed if the current temperature is between the preset temperature in the Standard menu and the Delta T limit value If the temperature leaves this range during the centrifugation run an error message wil...

Страница 45: ...he Save function will also be selected Select the button Activate code Enter a four digit code and confirm the entry The lock is now active The symbol will be displayed in the status line and the lock...

Страница 46: ...ge It enables the optimum adaptation of the centrifuge to its specific area of application Fig 20 Menu Setup Function Open lid after run The automatic lid opening function ensures that the lid opens w...

Страница 47: ...erature can be displayed in C Celsius or F Fahrenheit Language The control system can be used in various language versions If a language is selected by mistake it can be changed on any screen as follo...

Страница 48: ...e entered when the cursor flashes in the text field Turn the function knob in order to select a character and press it to confirm the selection Then press the knob again in order to enter the next cha...

Страница 49: ...u The curve editor will be displayed Select a curve number between 20 and 29 in the input field Curve If the curve number is already used the stored curve will be displayed The input field Int is used...

Страница 50: ...e since the necessary slope 0 03 min 1 rpm which means that it is beyond the defined range Example 2 Start speed 0 min 1 rpm final speed 15000 min 1 rpm runtime 10 sec not possible since the necessary...

Страница 51: ...0_0 docx 114 1 Pos 147 100 Sigma 100 BA Zentrifugen Sigma Standardm odul e 060 Betrieb_Spi ncontr ol L S Spincontrol S Zent7 060 0030 0030 Programmbetri eb 27 m od_1405320235093_68 docx 193459 3 1 6 3...

Страница 52: ...to confirm the selection Then the next character can be selected Pressing the arrow button will delete the last character When the program name is complete select OK and confirm it The program will be...

Страница 53: ...list and confirm the selection by pressing the function knob Press the start button Or Open the process library select the desired program and press the start button The program will be executed and...

Страница 54: ...n in the Setup menu Fig 27 Program rotation function When a program is loaded while the program rotation function is active this program will be used as the start program for the rotation After the co...

Страница 55: ...ding on the centrifuge type partly standard External signal active DC 24V 0 5 A max part no 17701 Floating switch AC 250V max 6 A part no 17702 Pos 160 010 Universalmodul e Leerzeile 0 mod_12021162445...

Страница 56: ...rectly Fuses have tripped Reactivate temperature fuse see chapter 5 2 1 Type of connection Mains power switch off Switch mains power switch on Centrifuge cannot be started start key LED is not illumin...

Страница 57: ...cord from the socket Remove the plug see figure item 1 from the opening on the right side panel e g with a screwdriver Fig 29 Position of the opening for the emergency lid release First pull the red s...

Страница 58: ...centrifuges Allow to slow down Power off Allow to cool down Provide better ventilation only air cooled centrifuges Provide sufficient water throughput only water cooled centrifuges 46 49 Imbalance err...

Страница 59: ...ent of queries malfunctions or spare part enquiries From Germany Contact Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode Germany Tel 49 0 55 22 50 07 44 44 E mail support lab sigma z...

Страница 60: ...al stress e g by heat generators Pos 175 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 176 100 Sigma 100 BA Zentrifugen Sigma Standardm odul e 080 War tung und Instandhaltung 080...

Страница 61: ...m odul e 080 War tung und Instandhaltung 080 0010 0020 Zubeh r au er 8K 28 m od_1405320618247_68 docx 194845 3 1 8 1 2 Accessories CAUTION For the care of the accessories special safety measures must...

Страница 62: ...elfachtr ger au er Basic 28 mod_1405320621063_68 docx 194873 3 1 8 1 3 Rotors buckets and carriers Rotors buckets and carriers are produced with the highest precision in order to withstand the perman...

Страница 63: ...t of the buckets and therefore the smooth operation of the centrifuge Load bearing bolts that are insufficiently greased may cause the centrifuge to stop due to an imbalance Clean the load bearing bol...

Страница 64: ...Grease the upper third of the rotor chamber with e g Vaseline Then let the rotor rotate for a few minutes at a moderate speed approx 2000 rpm The glass and metal particles will now collect at the gre...

Страница 65: ...Accessories Max temp C Min time min Max time min Max cycles Aluminium buckets 134 138 3 5 Aluminium rotors 134 138 3 5 Glass tubes 134 138 3 40 Polyallomer polycarbonate rectangular carriers 115 118 3...

Страница 66: ...o withstand this high level of stress high quality components were used during the production of the centrifuge Nevertheless wear cannot be excluded and it may not be visible from the outside Especial...

Страница 67: ...ment we are obliged to certify the harmlessness of all incoming goods For this purpose we require a declaration of decontamination The form must be filled in completely and signed by authorised and sp...

Страница 68: ...afe manner Please use the original packaging for the unit if at all possible If the product is dispatched to us in unsuitable packaging you will be charged the cost for returning it to you in new pack...

Страница 69: ...ong household waste You can return these centrifuges free of cost to Sigma Laborzentrifugen GmbH Ensure that the unit is decontaminated Fill in a declaration of decontamination see chapter 8 4 Return...

Страница 70: ...20 Osterode Germany Type 3 18KHS Connection requirements Electr connection Protection class IP code see name plate I 20 Power consumption kW Thermal circuit breaker A 1 01 10 0 at 220 240 V 50 60 Hz 1...

Страница 71: ...y of air 80 from 5 C up to 31 C with a linear decrease to 67 relative humidity of air at 35 C Maximum altitude 2 000 m above sea level Pos 210 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx...

Страница 72: ...www sigma zentrifugen de Pos 217 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 218 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 219 100 Sigma 100 BA Zentri...

Страница 73: ...l operating manual part no 0701202 Pos 221 100 Sigma 100 BA Zentrifugen Sigma Standardm odul e 110 Anhang 110 0020 Dr ehzahl Schwer efeld Diagr amm 28 m od_1405320705642_68 docx 195153 2 1 11 2 Speed...

Страница 74: ...ope of the fixed acceleration curves defines the time that is required to accelerate the rotor by 1 000 rpm Curve 9 is a special case compared to the other curves The centrifuge accelerates with maxim...

Страница 75: ...Appendix Version 08 2012 Rev 1 21 of 08 05 2020 sb 75 89 Translation of the original operating manual part no 0701202 Fig 36 Diagram of linear curves Fig 37 Diagram of quadratic curves Pos 224 010 Uni...

Страница 76: ...dition 1 16K Edition 12082 7 years 1 14 1 14K 12083 7 years 1 14 1 14K 12084 7 years 1 14 1 14K 12085 7 years 1 14 1 14K 12092 5 years 20x 1 14 1 14K 12093 5 years 20x 1 14 1 14K 12094 5 years 20x 1 1...

Страница 77: ...1 4 4 1 1 Acetone C3H6O 100 1 1 4 1 1 4 4 1 4 1 Acrylonitrile C3H3N 100 1 1 4 3 3 4 4 4 1 4 1 Allyl alcohol C3H6O 96 1 3 3 2 2 2 2 4 1 1 1 Aluminium chloride AlCl3 saturated 1 3 2 4 1 1 1 1 4 Alumini...

Страница 78: ...iaminoethane C2H8N2 100 1 1 3 1 1 3 4 1 1 1 Diesel fuel 100 1 1 3 1 1 1 3 1 1 1 Dimethyl formamide DMF C3D7NO 100 1 1 4 1 1 4 3 1 3 1 Dimethyl sulfoxide DMSO C2H6SO 100 1 2 4 1 1 4 4 1 1 Dimethylanili...

Страница 79: ...2 4 1 1 3 1 3 1 2 1 Methyl benzene C7H8 100 3 1 4 1 3 4 4 4 1 4 1 Methyl ethyl ketone MEK C4H8O 100 1 1 4 1 1 4 4 4 1 4 1 Methylene chloride CH2Cl2 100 4 3 4 3 3 4 4 4 1 1 Mineral oil 100 1 1 1 1 1 1...

Страница 80: ...30 1 1 4 1 1 1 1 1 1 2 4 Sodium hydroxide NaOH 50 1 1 4 1 1 1 1 1 2 4 Sodium sulfate Na2SO4 10 1 1 1 1 1 1 1 1 1 1 1 Spirits C2H6O 96 1 1 1 1 1 1 1 3 1 1 Styrene C8H8 100 4 1 4 1 3 4 4 1 4 1 Sulphuric...

Страница 81: ...ev 1 21 of 08 05 2020 sb 81 89 Translation of the original operating manual part no 0701202 Pos 229 100 Sigma 110 BA Zentrifugen Sigma Proj ekte 3 18KS 3 18KH S 110 Anhang 110 0060 Konformit tserkl ru...

Страница 82: ...Refrigerated Centrifuge with Heating System Sigma 3 18KHS 11 Appendix 82 89 Version 08 2012 Rev 1 21 of 08 05 2020 sb Translation of the original operating manual part no 0701202...

Страница 83: ...d Centrifuge with Heating System Sigma 3 18KHS 11 Appendix Version 08 2012 Rev 1 21 of 08 05 2020 sb 83 89 Translation of the original operating manual part no 0701202 11 7 Declaration of conformity C...

Страница 84: ...ated Centrifuge with Heating System Sigma 3 18KHS 11 Appendix 84 89 Version 08 2012 Rev 1 21 of 08 05 2020 sb Translation of the original operating manual part no 0701202 Ende der Liste f r Textm arke...

Страница 85: ...trifugation monitoring 43 Centrifugation of infectious toxic radioactive or pathogenic substances 19 Centrifugation principle 13 Centrifugation with low capacity 31 Centrifugation with vessels of vari...

Страница 86: ...program 53 Explanation of the symbols and notes 16 Explosive substances 19 External signal 48 F Filling quantity refrigerant 70 Fire preventions 19 Form for the return of defective parts 67 Function...

Страница 87: ...11 12 No indication on the display 56 No power in the mains supply 56 Noise level 70 Nominal voltage 12 Notes on safety and hazards 9 Notes on transport 24 O Online download of forms 68 Open lid afte...

Страница 88: ...mechanical 18 Safety conscious work 17 Saving a program 52 Scope of supply 10 Selection display and modification of data34 Serial number 12 59 66 Service 66 Service contact 59 Service life 60 Service...

Страница 89: ...nit 47 Temperature value not reached 56 Thermal circuit breaker 70 Thermal stress 60 Time fine 47 Time range 70 Toxic substances 19 60 Transport safety device 25 Tubes 32 Type 12 70 Type of connection...

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