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ver.210702E

 

SHOWA DENKO K.K. (https://www.shodex.com/)

 

Operation Manual 

Shodex

 RSpak

 DC-613 

 

Please read this manual carefully to achieve the best and consistent column performance for a long time

 

 

 

Important Handling Instructions 

 

 Caution! 

 

 

· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column 

and understand their proper handling methods to prevent potential health hazards or death 
from occurring. 

 

· Please wear appropriate personal protective equipment such as lab goggles and gloves 

when handling organic solvents and acid and alkaline reagents. Avoid any direct physical 
contact to prevent chemical injuries.   

 

Before Using the Column 

(1) Please visually inspect the package and outside of the column for any damage.   

 

(2) Please check if product name and serial number written on the column package, column name tag, and     

enclosed Certificate of Analysis (CoA) are matching and correct.   

 

1. Introduction 

Thank you for purchasing the Shodex

 product. Shodex

 RSpak

 DC-613 is a high performance 

chromatography column which is specifically designed for the separations and analysis of sugars. A strong acid 
cation exchange type packing gel, prepared from a rigid resin of styrene divinylbenzene copolymer provides a 
mixed separation mode. The analytes are separated by a combination of ligand exchange and hydrophilic 
chromatography (HILIC) modes. It is capable of separating and analyzing disaccharides or trisaccharides from 
each other.   
 

2. Column Components   

 

 

                 

 

 

 
 
 
 
 
 
 
 
 
3. Column Specifications 

Product 

Code 

Product Name 

Column Size (mm) 

Particle 

Size (

μ

m) 

Theoretical Plate Number 

(Per Column) 

I.D. Length 

F7001003 

RSpak DC-613 

6.0 150   

 5.500 

F6700170 

RSpak DC-G 4A 

4.6  

10  10 

(Guard 

Column) 

 

Base Material 

: Spherical porous particles of styrene divinylbenzene copolymer modified with sulfo group

(counter ion: Na

2+

)

 

Column Housing  : SUS-316 
Screw Type 

: Internally-threaded type No.10-32 UNF 

Shipping Solvent : Water/Acetonitrile = 30/70 
 
 
 

Содержание RSpak DC-613

Страница 1: ...ificate of Analysis CoA are matching and correct 1 Introduction Thank you for purchasing the Shodex product Shodex RSpak DC 613 is a high performance chromatography column which is specifically designed for the separations and analysis of sugars A strong acid cation exchange type packing gel prepared from a rigid resin of styrene divinylbenzene copolymer provides a mixed separation mode The analyt...

Страница 2: ...hromatograms Filter the eluent with a 0 45 μm disposable filter to prevent the problems Attention Whenever water is required use ultra pure water freshly generated by a water purification system or water from a newly opened HPLC grade distilled water bottle Use HPLC grade organic solvent whenever possible Solvents left in an opened bottle for a long time should not be used The content may have bee...

Страница 3: ...in front before the inlet of the analytical column 2 Make sure to insert the tubing all the way to the end fitting and secure it with the male nut It is important that there is no extra space between the tubing and the column side of the end fitting Presence of an extra space will let the sample to spread out and may result in wide peaks 3 Set the initial flow rate at less than 0 5 mL min and star...

Страница 4: ...ector Cleaning Method Insoluble components that block the column inlet may be removed by reversing the flow direction i e introducing the eluent from the column outlet with flow rate at less than half of the recommended flow rate Regeneration Method Presence of cations other than sodium ion in the sample may replace the sodium ion attached to the sulfo functional group This can change the separati...

Страница 5: ...late Number N 2 Asymmetry Factor Fas 10 Additional Warnings 1 Do not remove end fittings 2 Do not make a strong impact on the column Do not drop or hit the column on a hard surface Refer to the Shodex website https www shodex com for product details and their applications For additional assistance contact the distributor from whom you purchased the column or contact your regional Shodex support of...

Страница 6: ...anyone other than person or firm designated by Showa Denko K K 3 3 If the Shodex product is disposed of 3 4 If the Shodex product is resold by the user without giving prior written notice to Showa Denko K K 3 5 If the performance of the Shodex product is not conform to the specification of the attached CERTIFICATE OF ANALYSIS due to any of the reasons below a Computer virus b Impurities contained ...

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