Shodex OHpak LB-800 Series Скачать руководство пользователя страница 1

ver.211115E

 

SHOWA DENKO K.K. (https://www.shodex.com/)

 

Operation Manual 

Shodex

 OHpak

 LB-800 series 

 

Please read this manual carefully to achieve the best and consistent column performance for a long time

 

 
 

Important Handling Instructions 

 

 Caution! 

 

 

· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column 

and understand their proper handling methods to prevent potential health hazards or death 
from occurring. 

 

· Please wear appropriate personal protective equipment such as lab goggles and gloves 

when handling organic solvents and acid and alkaline reagents. Avoid any direct physical 
contact to prevent chemical injuries.   

 

Before Using the Column 

(1) Please visually inspect the package and outside of the column for any damage.   

 

(2) Please check if product name and serial number written on the column package, column name tag, and     

enclosed Certificate of Analysis (CoA) are matching and correct.   

 

1. Introduction 

Thank you for purchasing the Shodex™ product. Shodex

 OHpak

 LB-800 series is a size exclusion 

chromatography column used with aqueous solvents. The column series is suitable for the separations of 
various water-soluble high molecular weight polymers, proteins, and/or oligomers, and molecular weight 
distribution analysis of high molecular weight polymers. Successfully reduced baseline noise in the light 
scattering (LS) detector by suppressing the column bleeding as much as possible made it to provide accurate 
molecular weight measurements. The columns with different exclusion limits are available. Please select 
columns with target molecular weight ranges (see below) that meet the molecular weight range of your samples.   
 

2. Column Components

                 

 

 
 
 
 
 
 
 
 
 
 
 
3. Column Specifications 

Product 

Code 

Product Name 

Column Size 

(mm) 

Particle 

Size 

(

μ

m) 

Theoretical 

Plate Number

 

(Per Column) 

Target Molecular 

Weight Range

*1

 

Exclusion 

Limit

*1

 

LS Noise 

(V)

 *3

 

I.D. Length 

 

F6429206 

OHpak LB-802.5 

8.0 300 

 

 16,000 

500 - 10,000 

    10,000 

 5.0 x 10

-5

 

F6429201 

OHpak LB-803

 8.0 300 

 

 16,000   

1,000 - 100,000 

  100,000 

 2.0 x 10

-5

 

F6429204 

OHpak LB-804

 8.0 300  10 

 16,000 

5,000 - 400,000 

1,000,000 

 5.0 x 10

-5

 

F6429203 

OHpak LB-805

 8.0 300  13 

 12,000 

100,000 - 1,000,000 

(4,000,000)

*2

 

 5.0 x 10

-5

 

F6429205 

OHpak LB-806

 8.0 300  13 

 12,000 

100,000 - (20,000,000)

*2

 (20,000,000)

*2

 

 5.0 x 10

-5

 

F6429202 

OHpak LB-806M

 8.0  300 

13 

 12,000 

500 - (20,000,000)

*2

 (20,000,000)

*2

 

 2.0 x 10

-5

 

F6709434 

OHpak LB-G 6B

 6.0 

 

50 13 

(Guard 

Column) 

 

LB-806M is a mixed-gel column, suitable for the analysis of wide molecular weight ranges.   
 

Содержание OHpak LB-800 Series

Страница 1: ...water soluble high molecular weight polymers proteins and or oligomers and molecular weight distribution analysis of high molecular weight polymers Successfully reduced baseline noise in the light scattering LS detector by suppressing the column bleeding as much as possible made it to provide accurate molecular weight measurements The columns with different exclusion limits are available Please se...

Страница 2: ...emain on the column solvent replacement after their use takes a longer time than replacement from general solvents The replacement time can be shorten by using 30 50 v v methanol Attention Use the column within above stated flow rate pressure and temperature ranges Using the column outside the given range may damage the column and lower its performance When using a mixture of salt solution and org...

Страница 3: ...dilute samples If this is difficult use a solvent which has a composition that is as close as possible to the eluent s composition but which fully dissolves or dilutes the sample 2 Filter the sample solution using disposable 0 45 μm filter to prevent the column from clogging or deteriorating 3 To prepare a sample with molecular weight larger than 1 000 000 first allow the sample to stand in the el...

Страница 4: ...3 Set the initial flow rate at less than 0 3 mL min and start the system If using the column at an elevated temperature keep a low flow rate until the temperature of the column reaches to the set temperature and then gradually increase the flow rate to the desired 4 Multiple number of columns can be connected in series When connecting multiple columns with each having different exclusion limits se...

Страница 5: ...move the guard column and check the performance of the analytical column alone If the problem is solved most likely the cause is from the guard column In this case clean the guard column If the problem is not solved by removing the guard column clean both guard and analytical columns Make sure to clean the guard and the analytical columns separately When washing the column let the washing solution...

Страница 6: ...late Number N 2 Asymmetry Factor Fas 10 Additional Warnings 1 Do not remove end fittings 2 Do not make a strong impact on the column Do not drop or hit the column on a hard surface Refer to the Shodex website https www shodex com for product details and their applications For additional assistance contact the distributor from whom you purchased the column or contact your regional Shodex support of...

Страница 7: ...anyone other than person or firm designated by Showa Denko K K 3 3 If the Shodex product is disposed of 3 4 If the Shodex product is resold by the user without giving prior written notice to Showa Denko K K 3 5 If the performance of the Shodex product is not conform to the specification of the attached CERTIFICATE OF ANALYSIS due to any of the reasons below a Computer virus b Impurities contained ...

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