6. Type a name for the method and then press
Enter
.
7. Click
Next
.
8. On the
Ion Source Parameters
tab, verify the values, editing them if necessary, and then click
Next
.
9. On the
TOF MS
tab, verify the values, editing them if necessary, and then click
Finish
.
Tip!
If required, users can further edit the acquisition method using the
Acquisition Method Editor
.
In
Acquire
mode, click
File > Open
and then open the method that was created using the
Method
Wizard
.
Next steps: The newly created acquisition method can now be used to acquire data for preliminary analysis.
Scan Techniques
The system is a versatile and reliable system for performing liquid chromatography mass spectrometry analysis
on liquid sample streams to identify, quantify, and examine compounds.
The system uses the following mass spectrometry techniques to analyze samples:
• Two modes of single mass spectrometry (MS):
• Quadrupole-based single mass spectrometry (for Q1 calibration only)
• Time-of-flight-based single mass spectrometry
• Two modes of tandem mass spectrometry (MS/MS):
• Product ion mass spectrometry
• Precursor ion mass spectrometry
Single Mass Spectrometry
Single mass spectrometry (MS) is used to analyze charged molecules to find the molecular weight and amount of
detected ions. Individual ions detected by MS can indicate the presence of a target analyte.
Quadrupole-Based Single Mass Spectrometry
In a quadrupole-based single mass spectrometry (Q1 MS) scan, the system functions as a traditional quadrupole
mass spectrometer. In this mode, the system generates single mass spectrometric information using the first
quadrupole (Q1) section of the instrument.
TripleTOF
®
5600/5600+ System
System User Guide
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RUO-IDV-05-7040-B
Operating Instructions — Acquisition Methods
