An acquisition method consists of experiments and periods. Use the Acquisition Method Editor to create a sequence
of periods and experiments for the instrument and devices.
An acquisition method consists of the method for the mass spectrometer and for liquid chromatography (LC)
devices. Users can easily create an acquisition method using the Method Wizard.
The Acquisition Method Editor can also be used to create acquisition methods and to add a sequence of periods
and experiments for the instrument and devices.
Use the SWATH
®
acquisition feature, available in both the Method Wizard and the Acquisition Method Editor, to
create SWATH
®
acquisition methods. Also, SWATH
®
Variable width window methods can be created using the
Method Wizard or Acquisition Method Editor. For more information, refer to the
Advanced User Guide
,
Analyst
®
Help, and Method Wizard Help.
We recommend that only users who are proficient in method development create or modify acquisition and
quantitation methods. Refer to the
Laboratory Director’s Guide
for more information about roles and security.
Create an Acquisition Method Using the Acquisition
Method Editor
Tip!
If users are creating a new acquisition method file from an existing file, then some or all of the peripheral
device methods in the acquisition method might be used.
Only devices configured in the active hardware profile appear in the Acquisition method pane. Any devices added
to the hardware profile must also be added to existing acquisition methods. For more information about devices,
refer to the
Peripheral Devices Setup Guide
.
1. Make sure that a hardware profile containing the mass spectrometer and peripheral devices is active.
2. On the Navigation bar, under
Acquire
, double-click
Build Acquisition Method
.
3. Select a
Synchronization Mode
on the Acquisition Method Properties tab.
4. (Optional) Select the
Auto-Equilibration
check box and then type the required equilibration time, in minutes.
5. Click the
Mass Spec
icon in the Acquisition method pane.
6. Select a
Scan type
on the MS tab.
7. Type values in the fields as required. Refer to
.
8. On the Advanced MS tab, type values in the fields as required.
System User Guide
TripleTOF
®
5600/5600+ System
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Operating Instructions —
Acquisition Methods
