85032-536-64
Combi.jet 16847-CJ
2.5 Pour the sample to be tested into the funnel. For sample
volumes < 5 ml, charge with 10 to 20 ml sterile water or physio-
logical saline. For suctioning, reopen the stopcock (“6 o’clock”),
switch on the e.jet transfer pump and switch it off again at
the end of the filtration run or close the stopcock afterwards
(“9 o’clock”).
2.6 Remove the funnel and extract the membrane filter using
a flamed forceps.
2.7 Place the membrane filter on a nutrient pad set or on an agar
culture medium and subsequently incubate in a laboratory
incubator. When placing the membrane filter on the nutrient
pad set, make sure to avoid the inclusion of any air bubbles.
The membrane filter can additionally be used for gravimetric,
optical, chemical or other analyses.
2.8 Before storing the units, please rinse them with at least 70%
alcohol and allow the system components to air-dry.
2.9 Later analysis of the colony count can be simplified by mesh
embossed on the membrane.
Note: Prefiltration
Samples that have a high content of dirt and/or insoluble solids
should be prefiltered for the detection of bacteria. The prefiltration
adapter made of stainless steel (16807) allows prefiltration to be
conducted together with the actual membrane filtration in one work
session. Attach the adapter between the stainless steel funnel and
the lower part, fill to the brim with sterile water (this is to avoid
that any air inclusions impair filtration) and install a bacteriological
prefilter 11301-050 (8 μm pore size). Any coarse particles contained
in the sample to be tested will be retained by this prefilter which
takes a load off the membrane filter lying on the stainless steel
support and allows the colonies to grow without the adverse effects
of deposits.
The prefiltration adapter can similarly be flamed.
3. Cleaning and Care of Stainless Steel Equipment
To ensure a long service-life and functionality, it is necessary to
clean the units prior to first use and after each filtration.
To do so, proceed as follows:
1.
Unscrew the white coupling from the stainless steel arch.
2.
Clean all parts with warm water and gentle commercially
available laboratory cleaning agents (for metal, glass, plastic)
and soft brushes.
3.
Rinse off the parts with hot water and afterwards with distilled
water. Additional flushing with 70% alcohol accelerates the
drying process.
4.
Dry the parts in the drying oven. For drying, please use lint-free
towels that do not release fibers into the unit.
4. Specifications:
Material:
Stainless steel round arch:
Material 1.4301 (B.S. 304S31/AlSl 304)
Coupling: PVDF
Base support for membrane filter diameter 47 mm
Max. operating pressure:
Vacuum max. 2 bar overpressure
Autoclaving:
Max. 134°C, 30 min
Dry heat:
Max. 180°C, 30 min
1. Setting up the Combi.jet System
Assemble and set up the e.jet transfer pump according to the
operating instructions and equip it with tubing to drain off the
filtrate (When doing so, pay attention to the flow direction
indicated by the arrow on the black pump head!).
Now connect the outlet of Combi.jet by simply inserting the
white coupling into the hose barb on the Microsart
®
e.jet pump
(166MP-3).
2. Filtration
Stainless steel stopcocks on the lower parts allow the vacuum to
be switched on and off independently for each individual filtration
adapter, thereby minimizing the risk of secondary contamination.
The stainless steel supports serving as filter supports ensure that
residue or microbes are distributed evenly across the membrane
filter surface.
2.1 All parts of the system that come into contact with the sample
and filter membrane should be sterile. A variety of options are
available, depending on the material:
a) Autoclaving (stainless steel, glass and polycarbonates):
Assemble the units together so that a filter membrane is
securely clamped between the lower part and the funnel. Note
that the filter should not be clamped so firmly that steam is
prevented from entering into the clamp.
Setting the Two-way Stopcock to “6 O’clock”.
Cover the inlets
and outlets with aluminum foil and autoclave in accordance with
the specifications for the system components. After sterilization,
assemble the system securely back together and connect it to the
other system components.
Setting the Two-way Stopcock to “9 O’clock”.
a) Flaming (stainless steel only): This saves long waits in between
the individual filtration runs and will be described in detail
in the following.
2.2 Clean your laboratory bench and disinfect it with alcohol or
a commercially available disinfectant.
2.3 Set up your sterile culture media, e.g. nutrient pad sets in Petri
dishes or, alternately, agar in Petri dishes. Wet the nutrient pad
sets with 3.0–3.5 ml of sterile distilled or demineralized water.
For this purpose, we recommend our dosing syringe (16685-2)
with pre-installed sterile Minisart
®
(17597-K).
2.4 Place a Bunsen burner and a glass with some alcohol with
a forceps in it next to the autoclaved filtration unit.
a) Place the sterile-packaged membrane filters in easily accessible
proximity.
b) Switch on the e.jet transfer pump and remove the funnel.
c)
Flame the stainless steel support with the Bunsen burner.
To do so, open the stopcock (“6 o’clock”) and use a vacuum to
draw the flame through the support. Afterwards, re-close the
stopcock (“9 o’clock”).
d) Place the funnel back on to the lower part.
e) To speed up the cooling down of the filter unit and simplify
membrane filter insertion, rinse the unit with sterile water
(e.g. using a dosing syringe and an attached sterile Minisart
®
).
To suction off the water, open the stopcock (“6 o’clock”)
and close again (“9 o’clock”).
f)
Sterilize the forceps briefly over the flame and take the sterile
membrane filter (if applicable, with the yellow guard) out of its
packaging. Using one hand, remove the funnel from the lower
part of the unit and place the membrane filter (with the mesh
and/or yellow guard pointing up) in the middle of the support.
g) If the membrane filter is equipped with a yellow guard,
remove it before assembling the unit onto the upper part or
funnel. Place the funnel back into the lower part of the unit.
Typical Construction:
Microsart
®
e.jet pump (166MP-3) with Combi.jet manifold (16847-CJ) and pre-installed 100-ml stainless steel funnels
