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RotaChrom rCPC Operation Manual
All Rights Reserved by RotaChrom Technology LLC
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For normal operation of instrument, you should add the following: Flow rate, RPM, Inlet % (A,B,C,D),
Detector wavelength (A,B), Position (outlet), Pump and Rotation.
22.
figure: Required tabs for normal operation
After method development (table filled with timings and actions) make sure to save method changes.
Repeat these steps to write individual start, loop and stop methods. Before analysis set up a sequence.
Click on Sequence button and fill table as you can see below, then save your sequence.
23.
figure: Setup of sequence
Before you run an analysis you have to initiate devices (click on Method >Init Device or press Alt+I).
Ready state is useful for stabilization of devices (warming up, settle signals, etc.) and you can observe
this process at real time. You can change devices' parameters now through Devices' On-line
Configuration dialog (click on Method >Online configuration or press F10). If ready state is set it is
possible to start analysis. Choose Method >Run menu item or choose Run analysis menu item from
context menu to start analysis manually.
For more see Software manual for detailed usage of software.
Make sure that input liquids are connected to the gradient box and are in adequate amount and quality
for the operation. Connect waste container to fraction collector output 1 and connect fraction vessels
if necessary.
Normal operation of the instrument
The timing tab of the GUI is responsible for the order of the sequences. Normally it consists of 3 steps:
■
Start
–
startup of the instrument (detector wavelength, filling with stationary phase, spin up
of rotor)
■
Loop
–
injection cycle (injection, elution, fraction collection, extrusion)
■
Stop
–
stopping of the instrument
