Roche OMNI C, Инструкция по эксплуатации

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4 Measurement
4-4
Instructions for Use, Roche OMNI C, Rev. 4.0, December 2002
4.1.3 Sample handling
Whole blood
Withdraw whole blood samples using heparinized syringes, capillaries, or the microsampler.
Analyze the samples as soon as possible after sampling. Remove air bubbles from the sample
collection container immediately after the sampling procedure.
Immediately after withdrawing the sample with syringes, thoroughly mix the sample with
anticoagulant. This can be done by rolling the sample between both hands or shaking. Prop-
erly label the samples, following the standard documentation procedure.
• Samples that are measured within 15 minutes may be retained at room temperature.
• If unable to measure samples within 15 minutes, place them temporarily in ice water.
Complete the measurement within 30 minutes (but not after more than 60 minutes).
• Samples with a
P
O
2
level above 200 mmHg (26 kPa) should be collected in a glass con-
tainer if the measurement can not be performed within 15 minutes.
When using capillaries analyze samples for tHb, SO
2
and Hct measurements
immediately after sampling to ensure correct and accurate measurement results.
Despite proper sampling procedures, errors can arise in the blood gas analysis:
• due to insufficient mixing of the sample following removal and before the measurement
•
due to ambient air contamination caused by air bubbles that are not removed following
removal of the sample
• due to changes in metabolism in the sample
Serum
After the appearance of spontaneous clotting, process the sample in a centrifuge to separate
the cellular, solid components and the fibrin from the watery serum. Transfer the serum to
a suitable sample container and seal.
If it is necessary to store the sample, close the sample container tightly and cool it to 4 - 8 °C.
If a sample has been cooled, warm it to room temperature (15 - 33 °C) before analysis.
Plasma
Plasma samples are obtained by centrifuging heparinized whole blood, during which the cel-
lular components of the blood are removed from plasma.
Complete the analysis as quickly as possible.
If it is necessary to store the sample, close the sample container tightly and cool it to 4 -
8 °C. If a sample has been cooled, warm it to room temperature (15 - 33 °C) before analysis.
Plasma samples older than 1 hour must be re-centrifuged in order to remove fibrin clumps
that may have formed.